Literature DB >> 6409666

Immunochemical evidence for the presence of 5mC, 6mA and 7mG in human, Drosophila and mealybug DNA.

C W Achwal, C A Iyer, H S Chandra.   

Abstract

We have reported that production and characterization of antibodies highly specific to 5-methyl-cytosine (5mC) and the development of a sensitive immunochemical method for the detection of 5mC in DNA [FEBS Lett. (1982) 150, 469]. Extension of this method to two other modified bases, 6-methyladenine (6mA) and 7-methylguanine (7mG), is reported here. By use of this immunochemical approach, we are able to detect 5mC, 6mA and 7mG in human and Drosophila DNA and confirm their presence in the DNA of two mealybug species.

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Year:  1983        PMID: 6409666     DOI: 10.1016/0014-5793(83)80612-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN: 0014-5793            Impact factor:   4.124


  28 in total

1.  Cytosine methylation is not involved in the heterochromatization of the paternal genome of mealybug Planococcus citri.

Authors:  G Buglia; V Predazzi; M Ferraro
Journal:  Chromosome Res       Date:  1999       Impact factor: 5.239

2.  The relationship between DNA methylation and chromosome imprinting in the coccid Planococcus citri.

Authors:  S Bongiorni; O Cintio; G Prantera
Journal:  Genetics       Date:  1999-04       Impact factor: 4.562

3.  Detection of a CpA methylase in an insect system: characterization and substrate specificity.

Authors:  C Devajyothi; V Brahmachari
Journal:  Mol Cell Biochem       Date:  1992-03-25       Impact factor: 3.396

4.  Mutations in the Drosophila melanogaster gene encoding S-adenosylmethionine synthetase [corrected] suppress position-effect variegation.

Authors:  J Larsson; J Zhang; A Rasmuson-Lestander
Journal:  Genetics       Date:  1996-06       Impact factor: 4.562

Review 5.  Identifying 5-methylcytosine and related modifications in DNA genomes.

Authors:  T Rein; M L DePamphilis; H Zorbas
Journal:  Nucleic Acids Res       Date:  1998-05-15       Impact factor: 16.971

Review 6.  In praise of mealybugs.

Authors:  Vani Brahmachari; Surbhi Kohli; Parul Gulati
Journal:  J Genet       Date:  2018-06       Impact factor: 1.166

Review 7.  Epigenetics with special reference to the human X chromosome inactivation and the enigma of Drosophila DNA methylation.

Authors:  Deepti Deobagkar
Journal:  J Genet       Date:  2018-06       Impact factor: 1.166

Review 8.  N6-Methyladenine: A Conserved and Dynamic DNA Mark.

Authors:  Zach Klapholz O'Brown; Eric Lieberman Greer
Journal:  Adv Exp Med Biol       Date:  2016       Impact factor: 2.622

9.  Detection of DNA Methylation in Genomic DNA by UHPLC-MS/MS.

Authors:  Konstantinos Boulias; Eric Lieberman Greer
Journal:  Methods Mol Biol       Date:  2021

10.  Characterization and DNA methylation modulatory activity of gold nanoparticles synthesized by Pseudoalteromonas strain.

Authors:  Yugandhara M Patil; Shriram N Rajpathak; Deepti D Deobagkar
Journal:  J Biosci       Date:  2019-03       Impact factor: 1.826

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