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Literature DB >> 673848

DNA-methylase from regenerating rat liver: purification and characterisation.

D Simon, F Grunert, U von Acken, H P Döring, H Kröger.

Abstract

DNA methylase has been purified 660-fold from nuclei from regenerating rat liver. The enzyme is able to methylate single stranded (ss) and double stranded (ds) DNA, the only reaction product being 5-methylcytosine. Previously unmethylated double stranded DNA from prokaryotes (M.luteus) as well as from eukaryotes (Ascaris suis) can serve as substrates. The synthetic copolymers (dG-dC)n . (dC-dG)n and (dG,dC)n are also methylated. While SV40 DNA is almost not methylated, PM2 DNA is a good substrate even in the supercoiled form. The enzyme methylates 1 in 17 bases in heterologous M.luteus DNA, but only 1 in 590 in homologous rat liver DNA. The high methylation level of M.luteus DNA, an analysis of the methylated pyrimidine isostichs and a preliminary dinucleotide analysis suggest that all the CpGs in a DNA can be methylated.

Entities: Chemical Species

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Year: 1978 PMID： 673848 PMCID： PMC342150 DOI： 10.1093/nar/5.6.2153

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

28 in total

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Authors: J DOSKOCIL; F SORM
Journal: Biochim Biophys Acta Date: 1962-06-11

2. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors: R G MARTIN; B N AMES
Journal: J Biol Chem Date: 1961-05 Impact factor: 5.157

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Authors: G J Russell; P M Walker; R A Elton; J H Subak-Sharpe
Journal: J Mol Biol Date: 1976-11 Impact factor: 5.469

4. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

5. The purine and pyrimidine composition of deoxypentose nucleic acids.

Authors: G R WYATT
Journal: Biochem J Date: 1951-05 Impact factor: 3.857

6. Electrophoresis of DNA in agarose gels. Optimizing separations of conformational isomers of double- and single-stranded DNAs.

Authors: P H Johnson; L I Grossman
Journal: Biochemistry Date: 1977-09-20 Impact factor: 3.162

7. A buoyant method for the determination of the superhelix density of closed circular DNA.

Authors: H B Gray; W B Upholt; J Vinograd
Journal: J Mol Biol Date: 1971-11-28 Impact factor: 5.469

8. Rare bases in animal DNA.

Authors: B F Vanyushin; S G Tkacheva; A N Belozersky
Journal: Nature Date: 1970-03-07 Impact factor: 49.962

9. Analysis of calf-thymus satellite DNA: evidence for specific methylation of cytosine in C-G sequences.

Authors: F Gautier; H Bünemann; L Grotjahn
Journal: Eur J Biochem Date: 1977-10-17

10. The sequence specificity of a mammalian DNA methylase.

Authors: M J Browne; J F Turnbull; E L McKay; R L Adams; R H Burdon
Journal: Nucleic Acids Res Date: 1977-04 Impact factor: 16.971

23 in total

1. Detection of a CpA methylase in an insect system: characterization and substrate specificity.

Authors: C Devajyothi; V Brahmachari
Journal: Mol Cell Biochem Date: 1992-03-25 Impact factor: 3.396

Review 2. Biological aspects of cytosine methylation in eukaryotic cells.

Authors: M Hergersberg
Journal: Experientia Date: 1991-12-01

3. Kinetic mechanisms and interaction of rat liver DNA methyltransferase with defined DNA substrates.

Authors: M Ruchirawat; J Noshari; J N Lapeyre
Journal: Mol Cell Biochem Date: 1987-07 Impact factor: 3.396

4. Differential activity of DNA methyltransferase in the life cycle of Chlamydomonas reinhardi.

Authors: H Sano; C Grabowy; R Sager
Journal: Proc Natl Acad Sci U S A Date: 1981-05 Impact factor: 11.205

5. Complete DNA methylation does not prevent polyoma and simian virus 40 virus early gene expression.

Authors: M Graessmann; A Graessmann; H Wagner; E Werner; D Simon
Journal: Proc Natl Acad Sci U S A Date: 1983-11 Impact factor: 11.205

6. DNA methyltransferase induced by frog virus 3.

Authors: D B Willis; R Goorha; A Granoff
Journal: J Virol Date: 1984-01 Impact factor: 5.103

7. Two DNA methyltransferases from murine erythroleukemia cells: purification, sequence specificity, and mode of interaction with DNA.

Authors: T H Bestor; V M Ingram
Journal: Proc Natl Acad Sci U S A Date: 1983-09 Impact factor: 11.205