Literature DB >> 12913414

A combined HNCA/HNCO experiment for 15N labeled proteins with 13C at natural abundance.

Eriks Kupce1, D R Muhandiram, Lewis E Kay.   

Abstract

A triple resonance NMR experiment is presented for the simultaneous recording of HNCA and HNCO data sets on (15)N, natural abundance (13)C samples. The experiment exploits the fact that transfers of magnetization from (15)N to (13)CO and from (15)N to (13)C(alpha) (and back) proceed independently for samples that are not enriched in (13)C. A factor of 2 in measuring time is gained by recording the two data sets simultaneously with no compromise in spectral quality. An application to a 0.5 mM (15)N labeled sample of protein-L is presented with all expected correlations observed in spectra recorded with a cryogenic probe at 500 MHz.

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Year:  2003        PMID： 12913414     DOI： 10.1023/a:1024985219764

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


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