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Literature DB >> 10508768

NMR spectroscopy of large molecules and multimolecular assemblies in solution.

Abstract

New strategies and technical advances in NMR spectroscopy and biochemical methods for isotope labeling have enabled solution NMR studies of biomacromolecular systems of 100 kDa and larger. Recent progress has been made, in particular, with techniques for sequential resonance assignments, novel approaches for the direct observation of hydrogen bonds in nucleic acids and proteins, and segmental isotope labeling.

Entities: Chemical

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Year: 1999 PMID： 10508768 DOI： 10.1016/s0959-440x(99)00011-1

Source DB: PubMed Journal: Curr Opin Struct Biol ISSN： 0959-440X Impact factor: 6.809

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Cited

47 in total

1. A method for incorporating dipolar couplings into structure calculations in cases of (near) axial symmetry of alignment.

Authors: G A Mueller; W Y Choy; N R Skrynnikov; L E Kay
Journal: J Biomol NMR Date: 2000-11 Impact factor: 2.835

2. Accurate and rapid docking of protein-protein complexes on the basis of intermolecular nuclear overhauser enhancement data and dipolar couplings by rigid body minimization.

Authors: G M Clore
Journal: Proc Natl Acad Sci U S A Date: 2000-08-01 Impact factor: 11.205

NMR spectroscopy of large molecules and multimolecular assemblies in solution.

1. A method for incorporating dipolar couplings into structure calculations in cases of (near) axial symmetry of alignment.

2. Accurate and rapid docking of protein-protein complexes on the basis of intermolecular nuclear overhauser enhancement data and dipolar couplings by rigid body minimization.

3. Evaluation of site-directed spin labeling for characterizing protein-ligand complexes using simulated restraints.

4. Automated protein fold determination using a minimal NMR constraint strategy.

5. Preparation, characterization, and NMR spectroscopy of encapsulated proteins dissolved in low viscosity fluids.

6. An isotope labeling strategy for methyl TROSY spectroscopy.

7. Role for NMR in structural genomics.

8. Characterization and prediction of linker sequences of multi-domain proteins by a neural network.

9. Protein structure by mechanical triangulation.

10. Stereospecific assignments of the isopropyl methyl groups of the membrane protein OmpX in DHPC micelles.