Literature DB >> 12904350

Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system.

Temitayo Ajayi1, Leonard R Allmond, Teiji Sawa, Jeanine P Wiener-Kronish.   

Abstract

We mapped the coding single nucleotide polymorphisms in four toxin genes-exoS, exoT, exoU, and exoY-of the Pseudomonas aeruginosa type III secretion system among several clinical isolates. We then used this information to design a multiplex PCR assay based on the simultaneous amplification of fragments of these genes. Eight strains of known genotype were used to test our multiplex PCR method, which showed 100% sensitivity and specificity in this small sample size. This assay appears to be promising for the rapid and accurate genotyping of the presence of these genes in clinical strains of P. aeruginosa.

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Year:  2003        PMID： 12904350      PMCID： PMC179785          DOI： 10.1128/JCM.41.8.3526-3531.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  39 in total

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Authors:  M L Henriksson; R Rosqvist; M Telepnev; H Wolf-Watz; B Hallberg
Journal:  Biochem J       Date:  2000-04-01       Impact factor: 3.857

2.  14-3-3 proteins are required for the inhibition of Ras by exoenzyme S.

Authors:  M L Henriksson; U Trollér; B Hallberg
Journal:  Biochem J       Date:  2000-08-01       Impact factor: 3.857

3.  Pseudomonas aeruginosa ExoT is a Rho GTPase-activating protein.

Authors:  R Krall; G Schmidt; K Aktories; J T Barbieri
Journal:  Infect Immun       Date:  2000-10       Impact factor: 3.441

4.  Pathogenesis of septic shock in Pseudomonas aeruginosa pneumonia.

Authors:  K Kurahashi; O Kajikawa; T Sawa; M Ohara; M A Gropper; D W Frank; T R Martin; J P Wiener-Kronish
Journal:  J Clin Invest       Date:  1999-09       Impact factor: 14.808

5.  Type III protein secretion is associated with death in lower respiratory and systemic Pseudomonas aeruginosa infections.

Authors:  A Roy-Burman; R H Savel; S Racine; B L Swanson; N S Revadigar; J Fujimoto; T Sawa; D W Frank; J P Wiener-Kronish
Journal:  J Infect Dis       Date:  2001-05-17       Impact factor: 5.226

6.  Comparison of the exoS gene and protein expression in soil and clinical isolates of Pseudomonas aeruginosa.

Authors:  M W Ferguson; J A Maxwell; T S Vincent; J da Silva; J C Olson
Journal:  Infect Immun       Date:  2001-04       Impact factor: 3.441

7.  How the Pseudomonas aeruginosa ExoS toxin downregulates Rac.

Authors:  M Würtele; E Wolf; K J Pederson; G Buchwald; M R Ahmadian; J T Barbieri; A Wittinghofer
Journal:  Nat Struct Biol       Date:  2001-01

8.  Acquisition of expression of the Pseudomonas aeruginosa ExoU cytotoxin leads to increased bacterial virulence in a murine model of acute pneumonia and systemic spread.

Authors:  M Allewelt; F T Coleman; M Grout; G P Priebe; G B Pier
Journal:  Infect Immun       Date:  2000-07       Impact factor: 3.441

9.  ExoT of cytotoxic Pseudomonas aeruginosa prevents uptake by corneal epithelial cells.

Authors:  B A Cowell; D Y Chen; D W Frank; A J Vallis; S M Fleiszig
Journal:  Infect Immun       Date:  2000-01       Impact factor: 3.441

10.  The arginine finger domain of ExoT contributes to actin cytoskeleton disruption and inhibition of internalization of Pseudomonas aeruginosa by epithelial cells and macrophages.

Authors:  L Garrity-Ryan; B Kazmierczak; R Kowal; J Comolli; A Hauser; J N Engel
Journal:  Infect Immun       Date:  2000-12       Impact factor: 3.609
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  15 in total

1.  Presence of the exoU gene of Pseudomonas aeruginosa is correlated with cytotoxicity in MDCK cells but not with colonization in BALB/c mice.

Authors:  Hsi-Hsun Lin; Shiao-ping Huang; Hsiu-Chen Teng; Dar-Der Ji; Yao-Shen Chen; Ya-Lei Chen
Journal:  J Clin Microbiol       Date:  2006-10-18       Impact factor: 5.948

2.  Spread of multidrug-resistant Pseudomonas aeruginosa clones in a university hospital.

Authors:  Maria Koutsogiannou; Eleanna Drougka; Apostolos Liakopoulos; Eleni Jelastopulu; Efthimia Petinaki; Evangelos D Anastassiou; Iris Spiliopoulou; Myrto Christofidou
Journal:  J Clin Microbiol       Date:  2012-12-12       Impact factor: 5.948

3.  Use of a Multiplex Transcript Method for Analysis of Pseudomonas aeruginosa Gene Expression Profiles in the Cystic Fibrosis Lung.

Authors:  Alex H Gifford; Sven D Willger; Emily L Dolben; Lisa A Moulton; Dana B Dorman; Heather Bean; Jane E Hill; Thomas H Hampton; Alix Ashare; Deborah A Hogan
Journal:  Infect Immun       Date:  2016-09-19       Impact factor: 3.441

4.  Relationship of biofilm-forming ability of with swimming motility, twitching motility and virulence gene distribution.

Authors:  Jian Shui; Haichen Wang; Xiaoyan Tao; Changhang Min; Jun Li; Mingxiang Zou
Journal:  Zhejiang Da Xue Xue Bao Yi Xue Ban       Date:  2021-06-25

Review 5.  The type III secretion system of Pseudomonas aeruginosa: infection by injection.

Authors:  Alan R Hauser
Journal:  Nat Rev Microbiol       Date:  2009-09       Impact factor: 60.633

6.  Molecular Characterization of Carbapenemase-Producing Pseudomonas aeruginosa of Czech Origin and Evidence for Clonal Spread of Extensively Resistant Sequence Type 357 Expressing IMP-7 Metallo-β-Lactamase.

Authors:  Costas C Papagiannitsis; Matej Medvecky; Katerina Chudejova; Anna Skalova; Veronika Rotova; Petra Spanelova; Vladislav Jakubu; Helena Zemlickova; Jaroslav Hrabak
Journal:  Antimicrob Agents Chemother       Date:  2017-11-22       Impact factor: 5.191

7.  Co-existence of multidrug-resistant and -susceptible strains of Pseudomonas aeruginosa from a single clinical isolate.

Authors:  Krishna Mahida; Dong H Kwon
Journal:  Curr Microbiol       Date:  2009-12-29       Impact factor: 2.188

8.  Population structure of Pseudomonas aeruginosa from five Mediterranean countries: evidence for frequent recombination and epidemic occurrence of CC235.

Authors:  Makaoui Maatallah; Jihane Cheriaa; Amina Backhrouf; Aina Iversen; Hajo Grundmann; Thuy Do; Philippe Lanotte; Maha Mastouri; Mohamed Salem Elghmati; Fernando Rojo; Snoussi Mejdi; Christian G Giske
Journal:  PLoS One       Date:  2011-10-03       Impact factor: 3.240

9.  Differentiation in quinolone resistance by virulence genotype in Pseudomonas aeruginosa.

Authors:  Melissa Agnello; Annie Wong-Beringer
Journal:  PLoS One       Date:  2012-08-08       Impact factor: 3.240

10.  Diversity of Antimicrobial Resistance and Virulence Determinants in Pseudomonas aeruginosa Associated with Fresh Vegetables.

Authors:  Kashina Allydice-Francis; Paul D Brown
Journal:  Int J Microbiol       Date:  2012-11-20
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