Literature DB >> 12843084

Detection of Legionella pneumophila by real-time PCR for the mip gene.

Deborah A Wilson1, Belinda Yen-Lieberman, Udo Reischl, Steve M Gordon, Gary W Procop.   

Abstract

A real-time PCR assay for the mip gene of Legionella pneumophila was tested with 27 isolates of L. pneumophila, 20 isolates of 14 other Legionella species, and 103 non-Legionella bacteria. Eight culture-positive and 40 culture-negative clinical specimens were tested. This assay was 100% sensitive and 100% specific for L. pneumophila.

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Year:  2003        PMID： 12843084      PMCID： PMC165371          DOI： 10.1128/JCM.41.7.3327-3330.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  27 in total

1.  Detection of Legionella pneumophila using a real-time PCR hybridization assay.

Authors:  A L Ballard; N K Fry; L Chan; S B Surman; J V Lee; T G Harrison; K J Towner
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

2.  Rapid identification of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation using real-time fluorescence PCR.

Authors:  U Reischl; H J Linde; M Metz; B Leppmeier; N Lehn
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

3.  Development of conventional and real-time PCR assays for detection of Legionella DNA in respiratory specimens.

Authors:  K Rantakokko-Jalava; J Jalava
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

4.  Trends in legionnaires disease, 1980-1998: declining mortality and new patterns of diagnosis.

Authors:  Andrea L Benin; Robert F Benson; Richard E Besser
Journal:  Clin Infect Dis       Date:  2002-10-14       Impact factor: 9.079

5.  Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR.

Authors:  N Wellinghausen; C Frost; R Marre
Journal:  Appl Environ Microbiol       Date:  2001-09       Impact factor: 4.792

6.  Direct detection of Legionella species from bronchoalveolar lavage and open lung biopsy specimens: comparison of LightCycler PCR, in situ hybridization, direct fluorescence antigen detection, and culture.

Authors:  R T Hayden; J R Uhl; X Qian; M K Hopkins; M C Aubry; A H Limper; R V Lloyd; F R Cockerill
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

7.  Enzyme-linked immunoassay for detection of PCR-amplified DNA of legionellae in bronchoalveolar fluid.

Authors:  D Jonas; A Rosenbaum; S Weyrich; S Bhakdi
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

8.  Comparison of the Binax Legionella urinary antigen enzyme immunoassay (EIA) with the Biotest Legionella Urin antigen EIA for detection of Legionella antigen in both concentrated and nonconcentrated urine samples.

Authors:  J A Domínguez; N Galí; P Pedroso; A Fargas; E Padilla; J M Manterola; L Matas
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

9.  Clinical utility of a monoclonal direct fluorescent reagent specific for Legionella pneumophila: comparative study with other reagents.

Authors:  P H Edelstein; K B Beer; J C Sturge; A J Watson; L C Goldstein
Journal:  J Clin Microbiol       Date:  1985-09       Impact factor: 5.948

10.  Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-color real-time PCR and melting curve analysis.

Authors:  Udo Reischl; Hans-Jörg Linde; Norbert Lehn; Olfert Landt; Kevin Barratt; Nele Wellinghausen
Journal:  J Clin Microbiol       Date:  2002-10       Impact factor: 5.948
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  30 in total

1.  Utility of PCR, Culture, and Antigen Detection Methods for Diagnosis of Legionellosis.

Authors:  Derrick J Chen; Gary W Procop; Sherilynn Vogel; Belinda Yen-Lieberman; Sandra S Richter
Journal:  J Clin Microbiol       Date:  2015-08-19       Impact factor: 5.948

2.  Multicenter comparison of molecular methods for detection of Legionella spp. in sputum samples.

Authors:  M A Bencini; A J C van den Brule; E C J Claas; M H A Hermans; W J G Melchers; G T Noordhoek; M M M Salimans; J Schirm; C Vink; A van der Zee; R Jansen
Journal:  J Clin Microbiol       Date:  2007-08-01       Impact factor: 5.948

3.  Comparison of five methods for extraction of Legionella pneumophila from respiratory specimens.

Authors:  Deborah Wilson; Belinda Yen-Lieberman; Udo Reischl; Ilka Warshawsky; Gary W Procop
Journal:  J Clin Microbiol       Date:  2004-12       Impact factor: 5.948

4.  Quantitative detection of Legionella pneumophila in water samples by immunomagnetic purification and real-time PCR amplification of the dotA gene.

Authors:  M A Yáñez; C Carrasco-Serrano; V M Barberá; V Catalán
Journal:  Appl Environ Microbiol       Date:  2005-07       Impact factor: 4.792

Review 5.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

6.  Impacts of Hurricanes Katrina and Rita on the microbial landscape of the New Orleans area.

Authors:  C D Sinigalliano; M L Gidley; T Shibata; D Whitman; T H Dixon; E Laws; A Hou; D Bachoon; L Brand; L Amaral-Zettler; R J Gast; G F Steward; O D Nigro; R Fujioka; W Q Betancourt; G Vithanage; J Mathews; L E Fleming; H M Solo-Gabriele
Journal:  Proc Natl Acad Sci U S A       Date:  2007-05-08       Impact factor: 11.205

7.  Legionella feeleii serotype 2 pneumonia in a man with chronic lymphocytic leukemia: a challenging diagnosis.

Authors:  Marc O Siegel; Daniel P Fedorko; Steven K Drake; Leslie B Calhoun; Steven M Holland
Journal:  J Clin Microbiol       Date:  2010-03-31       Impact factor: 5.948

Review 8.  Diagnosis of Legionella infection in Legionnaires' disease.

Authors:  J W Den Boer; E P F Yzerman
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2004-12       Impact factor: 3.267

Review 9.  Diagnostic Accuracy of PCR Alone and Compared to Urinary Antigen Testing for Detection of Legionella spp.: a Systematic Review.

Authors:  Tomer Avni; Amir Bieber; Hefziba Green; Tali Steinmetz; Leonard Leibovici; Mical Paul
Journal:  J Clin Microbiol       Date:  2015-12-09       Impact factor: 5.948

10.  Molecular typing of a Legionella pneumophila outbreak in Ontario, Canada.

Authors:  Matthew W Gilmour; Kathryn Bernard; Dobryan M Tracz; Adam B Olson; Cindi R Corbett; Tamara Burdz; Betty Ng; Deborah Wiebe; George Broukhanski; Peter Boleszczuk; Patrick Tang; Frances Jamieson; Gary Van Domselaar; Francis A Plummer; Jody D Berry
Journal:  J Med Microbiol       Date:  2007-03       Impact factor: 2.472
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