Literature DB >> 10835024

Rapid identification of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation using real-time fluorescence PCR.

U Reischl1, H J Linde, M Metz, B Leppmeier, N Lehn.   

Abstract

A duplex LightCycler PCR assay targeting the mecA gene and a Staphylococcus aureus-specific marker was used to test 165 S. aureus strains and 80 strains of other bacterial species. Within an assay time of 60 min plus 10 min for sample preparation, S. aureus as well as the presence or absence of the mecA gene was correctly identified.

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Year:  2000        PMID： 10835024      PMCID： PMC86833          DOI： 10.1128/JCM.38.6.2429-2433.2000

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

1.  Correlation of oxacillin MIC with mecA gene carriage in coagulase-negative staphylococci.

Authors:  Z Hussain; L Stoakes; V Massey; D Diagre; V Fitzgerald; S El Sayed; R Lannigan
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Methicillin-resistant staphylococci.

Authors:  M BARBER
Journal:  J Clin Pathol       Date:  1961-07       Impact factor: 3.411

3.  Phenotypical and genotypical characterization of epidemic clumping factor-negative, oxacillin-resistant Staphylococcus aureus.

Authors:  A Schwarzkopf; H Karch; H Schmidt; W Lenz; J Heesemann
Journal:  J Clin Microbiol       Date:  1993-09       Impact factor: 5.948

4.  PCR-based detection of mycobacteria in sputum samples using a simple and reliable DNA extraction protocol.

Authors:  U Reischl; M Pulz; W Ehret; H Wolf
Journal:  Biotechniques       Date:  1994-11       Impact factor: 1.993

5.  Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction.

Authors:  K Murakami; W Minamide; K Wada; E Nakamura; H Teraoka; S Watanabe
Journal:  J Clin Microbiol       Date:  1991-10       Impact factor: 5.948

6.  Methicillin-resistant Staphylococcus aureus in Europe.

Authors:  A Voss; D Milatovic; C Wallrauch-Schwarz; V T Rosdahl; I Braveny
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1994-01       Impact factor: 3.267

7.  Methicillin-resistant Staphylococcus aureus in U.S. hospitals, 1975-1991.

Authors:  A L Panlilio; D H Culver; R P Gaynes; S Banerjee; T S Henderson; J S Tolson; W J Martone
Journal:  Infect Control Hosp Epidemiol       Date:  1992-10       Impact factor: 3.254

8.  Failure of rapid agglutination methods to detect oxacillin-resistant Staphylococcus aureus.

Authors:  P J Ruane; M A Morgan; D M Citron; M E Mulligan
Journal:  J Clin Microbiol       Date:  1986-09       Impact factor: 5.948

9.  Coagulase-negative staphylococci resistant to beta-lactam antibiotics in vivo produce penicillin-binding protein 2a.

Authors:  H F Chambers
Journal:  Antimicrob Agents Chemother       Date:  1987-12       Impact factor: 5.191

10.  Choice of a routine method for detecting methicillin-resistance in staphylococci.

Authors:  F Wallet; M Roussel-Delvallez; R J Courcol
Journal:  J Antimicrob Chemother       Date:  1996-05       Impact factor: 5.790
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  73 in total

1.  Sporadic cases of Staphylococcus aureus organisms negative for a species-specific 442-base pair chromosomal fragment.

Authors:  Katja Sütterlin; Ralf Englert; Thorsten Schmidt-Wieland; Jürgen Schmitt; Udo Reischl; Norbert Lehn
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

2.  Clinical Staphylococcus aureus isolate negative for the Sa442 fragment.

Authors:  Corné H W Klaassen; Hanneke A de Valk; Alphons M Horrevorts
Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948

Review 3.  Next-generation and whole-genome sequencing in the diagnostic clinical microbiology laboratory.

Authors:  W M Dunne; L F Westblade; B Ford
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2012-06-08       Impact factor: 3.267

4.  Evaluation of the BD GeneOhm assay using the rotor-gene 6000 platform for rapid detection of methicillin-resistant Staphylococcus aureus from pooled screening swabs.

Authors:  Melvyn Howard Smith; Julian Hodgson; Ian Joseph Eltringham
Journal:  J Clin Microbiol       Date:  2010-10-20       Impact factor: 5.948

5.  Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay.

Authors:  David K Warren; Robert S Liao; Liana R Merz; Michael Eveland; W Michael Dunne
Journal:  J Clin Microbiol       Date:  2004-12       Impact factor: 5.948

6.  Accuracy of antibiotyping using standard antibiograms compared with 16S-23S ribosomal spacer PCR for diagnosis of MRSA.

Authors:  F Daxboeck; S Mustafa; O Assadian; H Heinzl; M Stadler; A M Hirschl; W Koller
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2005-09       Impact factor: 3.267

7.  Shortcut method for extraction of Staphylococcus aureus DNA from blood cultures and conventional cultures for use in real-time PCR assays.

Authors:  D Nicholas Adams
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

8.  New strains of community-acquired methicillin-resistant Staphylococcus aureus with Panton-Valentine leukocidin causing an outbreak of severe soft tissue infection in a football team.

Authors:  M Müller-Premru; B Strommenger; N Alikadic; W Witte; A W Friedrich; K Seme; N Svent Kucina; D Smrke; V Spik; M Gubina
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2005-12       Impact factor: 3.267

Review 9.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

10.  Detection of staphylococcal cassette chromosome mec-associated DNA segments in multiresistant methicillin-susceptible Staphylococcus aureus (MSSA) and identification of Staphylococcus epidermidis ccrAB4 in both methicillin-resistant S. aureus and MSSA.

Authors:  Anna C Shore; Angela S Rossney; Brian O'Connell; Celine M Herra; Derek J Sullivan; Hilary Humphreys; David C Coleman
Journal:  Antimicrob Agents Chemother       Date:  2008-10-13       Impact factor: 5.191
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