Literature DB >> 12354888

Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-color real-time PCR and melting curve analysis.

Udo Reischl1, Hans-Jörg Linde, Norbert Lehn, Olfert Landt, Kevin Barratt, Nele Wellinghausen.   

Abstract

A dual-color LightCycler PCR assay targeting the 16S rDNA gene of Legionella spp. was established. By using two pairs of hybridization probes, Legionella spp. and Legionella pneumophila could be detected and differentiated simultaneously. With 26 culture-positive and 42 culture-negative respiratory specimens from patients with atypical pneumonia, 100% sensitivity and specificity was observed for L. pneumophila.

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Year:  2002        PMID： 12354888      PMCID： PMC130908          DOI： 10.1128/JCM.40.10.3814-3817.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Detection of Legionella pneumophila using a real-time PCR hybridization assay.

Authors:  A L Ballard; N K Fry; L Chan; S B Surman; J V Lee; T G Harrison; K J Towner
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

Review 2.  Classification of the genus Legionella.

Authors:  R F Benson; B S Fields
Journal:  Semin Respir Infect       Date:  1998-06

3.  Hospital characteristics associated with colonization of water systems by Legionella and risk of nosocomial legionnaires' disease: a cohort study of 15 hospitals.

Authors:  J L Kool; D Bergmire-Sweat; J C Butler; E W Brown; D J Peabody; D S Massi; J C Carpenter; J M Pruckler; R F Benson; B S Fields
Journal:  Infect Control Hosp Epidemiol       Date:  1999-12       Impact factor: 3.254

4.  Use of the polymerase chain reaction to detect Legionella DNA in urine and serum samples from patients with pneumonia.

Authors:  D R Murdoch; E J Walford; L C Jennings; G J Light; M I Schousboe; A Y Chereshsky; S T Chambers; G I Town
Journal:  Clin Infect Dis       Date:  1996-09       Impact factor: 9.079

5.  Delay in appropriate therapy of Legionella pneumonia associated with increased mortality.

Authors:  C H Heath; D I Grove; D F Looke
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1996-04       Impact factor: 3.267

6.  Detection of Legionella species in respiratory specimens using PCR with sequencing confirmation.

Authors:  J L Cloud; K C Carroll; P Pixton; M Erali; D R Hillyard
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

7.  Direct detection of Legionella species from bronchoalveolar lavage and open lung biopsy specimens: comparison of LightCycler PCR, in situ hybridization, direct fluorescence antigen detection, and culture.

Authors:  R T Hayden; J R Uhl; X Qian; M K Hopkins; M C Aubry; A H Limper; R V Lloyd; F R Cockerill
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

8.  Sequence-based classification scheme for the genus Legionella targeting the mip gene.

Authors:  R M Ratcliff; J A Lanser; P A Manning; M W Heuzenroeder
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

9.  Comparison of the Binax Legionella urinary antigen enzyme immunoassay (EIA) with the Biotest Legionella Urin antigen EIA for detection of Legionella antigen in both concentrated and nonconcentrated urine samples.

Authors:  J A Domínguez; N Galí; P Pedroso; A Fargas; E Padilla; J M Manterola; L Matas
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

Review 10.  Legionnaires' disease: clinical, epidemiological, and public health perspectives.

Authors:  R F Breiman; J C Butler
Journal:  Semin Respir Infect       Date:  1998-06
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  27 in total

1.  Real-time PCR assay targets the 23S-5S spacer for direct detection and differentiation of Legionella spp. and Legionella pneumophila.

Authors:  Bjorn L Herpers; Bartelt M de Jongh; Kim van der Zwaluw; Erik J van Hannen
Journal:  J Clin Microbiol       Date:  2003-10       Impact factor: 5.948

2.  Multicenter comparison of molecular methods for detection of Legionella spp. in sputum samples.

Authors:  M A Bencini; A J C van den Brule; E C J Claas; M H A Hermans; W J G Melchers; G T Noordhoek; M M M Salimans; J Schirm; C Vink; A van der Zee; R Jansen
Journal:  J Clin Microbiol       Date:  2007-08-01       Impact factor: 5.948

Review 3.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

4.  Development and evaluation of a real-time PCR assay targeting the type III secretion system of Burkholderia pseudomallei.

Authors:  Ryan T Novak; Mindy B Glass; Jay E Gee; Daniel Gal; Mark J Mayo; Bart J Currie; Patricia P Wilkins
Journal:  J Clin Microbiol       Date:  2006-01       Impact factor: 5.948

5.  Culture-independent identification of the source of an infection by direct amplification and sequencing of Legionella pneumophila DNA from a clinical specimen.

Authors:  Paul Christian Lück; Christa Ecker; Udo Reischl; Hans-Jörg Linde; Roland Stempka
Journal:  J Clin Microbiol       Date:  2007-07-25       Impact factor: 5.948

Review 6.  Current and emerging Legionella diagnostics for laboratory and outbreak investigations.

Authors:  Jeffrey W Mercante; Jonas M Winchell
Journal:  Clin Microbiol Rev       Date:  2015-01       Impact factor: 26.132

Review 7.  Diagnosis of Legionella infection in Legionnaires' disease.

Authors:  J W Den Boer; E P F Yzerman
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2004-12       Impact factor: 3.267

8.  Two-step scheme for rapid identification and differentiation of Legionella pneumophila and non-Legionella pneumophila species.

Authors:  Xiao-Yong Zhan; Lian-Qing Li; Chao-Hui Hu; Qing-Yi Zhu
Journal:  J Clin Microbiol       Date:  2009-12-09       Impact factor: 5.948

9.  Detection of Legionella pneumophila by real-time PCR for the mip gene.

Authors:  Deborah A Wilson; Belinda Yen-Lieberman; Udo Reischl; Steve M Gordon; Gary W Procop
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

Review 10.  Diagnostic Accuracy of PCR Alone and Compared to Urinary Antigen Testing for Detection of Legionella spp.: a Systematic Review.

Authors:  Tomer Avni; Amir Bieber; Hefziba Green; Tali Steinmetz; Leonard Leibovici; Mical Paul
Journal:  J Clin Microbiol       Date:  2015-12-09       Impact factor: 5.948
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