| Literature DB >> 17670926 |
M A Bencini1, A J C van den Brule, E C J Claas, M H A Hermans, W J G Melchers, G T Noordhoek, M M M Salimans, J Schirm, C Vink, A van der Zee, R Jansen.
Abstract
Legionellosis can be diagnosed by PCR using sputum samples. In this report, the methods of nine laboratories for 12 sputum samples with Legionella pneumophila and Legionella longbeachae are compared. We conclude that (i) liquefaction prevents PCR inhibition, (ii) the employed mip gene PCRs detected L. pneumophila only, and (iii) the 16S rRNA gene PCR detected both Legionella species and is preferred for the diagnosis of legionellosis.Entities:
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Year: 2007 PMID： 17670926 PMCID： PMC2045317 DOI： 10.1128/JCM.00505-07
Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948