Literature DB >> 11060093

Detection of Legionella pneumophila using a real-time PCR hybridization assay.

A L Ballard1, N K Fry, L Chan, S B Surman, J V Lee, T G Harrison, K J Towner.   

Abstract

A real-time PCR hybridization assay for Legionella pneumophila is described; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. The assay, including DNA extraction and confirmation of product identity, is completed within 90 min of receipt of a sample.

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Year:  2000        PMID： 11060093      PMCID： PMC87566     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Detection of Legionella spp. in bronchoalveolar lavage fluids by DNA amplification.

Authors:  B Jaulhac; M Nowicki; N Bornstein; O Meunier; G Prevost; Y Piemont; J Fleurette; H Monteil
Journal:  J Clin Microbiol       Date:  1992-04       Impact factor: 5.948

2.  Species-specific detection of Legionella pneumophila in water by DNA amplification and hybridization.

Authors:  M N Starnbach; S Falkow; L S Tompkins
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

3.  Use of real-time PCR and fluorimetry to detect lamivudine resistance-associated mutations in hepatitis B virus.

Authors:  P A Cane; P Cook; D Ratcliffe; D Mutimer; D Pillay
Journal:  Antimicrob Agents Chemother       Date:  1999-07       Impact factor: 5.191

4.  A Legionella pneumophila gene encoding a species-specific surface protein potentiates initiation of intracellular infection.

Authors:  N P Cianciotto; B I Eisenstein; C H Mody; G B Toews; N C Engleberg
Journal:  Infect Immun       Date:  1989-04       Impact factor: 3.441

5.  Use of polymerase chain reaction in an epidemiologic investigation of Pontiac fever.

Authors:  L A Miller; J L Beebe; J C Butler; W Martin; R Benson; R E Hoffman; B S Fields
Journal:  J Infect Dis       Date:  1993-09       Impact factor: 5.226

6.  Rapid detection of Legionella species in bronchoalveolar lavage fluids with the EnviroAmp Legionella PCR amplification and detection kit.

Authors:  H H Kessler; F F Reinthaler; A Pschaid; K Pierer; B Kleinhappl; E Eber; E Marth
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

7.  Construction of a DNA amplification assay for detection of Legionella species in clinical samples.

Authors:  G Lisby; R Dessau
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1994-03       Impact factor: 3.267

8.  Detection of Legionella DNA in human and guinea pig urine samples by the polymerase chain reaction.

Authors:  M Maiwald; M Schill; C Stockinger; J H Helbig; P C Lück; W Witzleb; H G Sonntag
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1995-01       Impact factor: 3.267

9.  Detection of mip gene by PCR for diagnosis of Legionnaires' disease.

Authors:  D S Lindsay; W H Abraham; R J Fallon
Journal:  J Clin Microbiol       Date:  1994-12       Impact factor: 5.948

10.  Non-culturable Legionella pneumophila associated with Acanthamoeba castellanii: detection of the bacterium using DNA amplification and hybridization.

Authors:  J Hay; D V Seal; B Billcliffe; J H Freer
Journal:  J Appl Bacteriol       Date:  1995-01
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  30 in total

1.  Novel PCR-probe assay for detection of and discrimination between Legionella pneumophila and other Legionella species in clinical samples.

Authors:  Anneke van Der Zee; Harold Verbakel; Caroline de Jong; Raymond Pot; Anneke Bergmans; Marcel Peeters; Peter Schneeberger; Joop Schellekens
Journal:  J Clin Microbiol       Date:  2002-03       Impact factor: 5.948

2.  Real-time PCR assay targets the 23S-5S spacer for direct detection and differentiation of Legionella spp. and Legionella pneumophila.

Authors:  Bjorn L Herpers; Bartelt M de Jongh; Kim van der Zwaluw; Erik J van Hannen
Journal:  J Clin Microbiol       Date:  2003-10       Impact factor: 5.948

3.  Multicenter comparison of molecular methods for detection of Legionella spp. in sputum samples.

Authors:  M A Bencini; A J C van den Brule; E C J Claas; M H A Hermans; W J G Melchers; G T Noordhoek; M M M Salimans; J Schirm; C Vink; A van der Zee; R Jansen
Journal:  J Clin Microbiol       Date:  2007-08-01       Impact factor: 5.948

4.  Quantitative detection of Legionella pneumophila in water samples by immunomagnetic purification and real-time PCR amplification of the dotA gene.

Authors:  M A Yáñez; C Carrasco-Serrano; V M Barberá; V Catalán
Journal:  Appl Environ Microbiol       Date:  2005-07       Impact factor: 4.792

5.  Integrated real-time PCR for detection and monitoring of Legionella pneumophila in water systems.

Authors:  Diaraf Farba Yaradou; Sylvie Hallier-Soulier; Sophie Moreau; Florence Poty; Yves Hillion; Monique Reyrolle; Janine André; Gabriel Festoc; Karine Delabre; François Vandenesch; Jerome Etienne; Sophie Jarraud
Journal:  Appl Environ Microbiol       Date:  2006-12-28       Impact factor: 4.792

6.  On-filter direct amplification of Legionella pneumophila for rapid assessment of its abundance and viability.

Authors:  Farag A Samhan; Tiffany M Stedtfeld; Hassan Waseem; Maggie R Williams; Robert D Stedtfeld; Syed A Hashsham
Journal:  Water Res       Date:  2017-05-13       Impact factor: 11.236

Review 7.  Current and emerging Legionella diagnostics for laboratory and outbreak investigations.

Authors:  Jeffrey W Mercante; Jonas M Winchell
Journal:  Clin Microbiol Rev       Date:  2015-01       Impact factor: 26.132

Review 8.  Diagnosis of Legionella infection in Legionnaires' disease.

Authors:  J W Den Boer; E P F Yzerman
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2004-12       Impact factor: 3.267

9.  Detection of Legionella pneumophila by real-time PCR for the mip gene.

Authors:  Deborah A Wilson; Belinda Yen-Lieberman; Udo Reischl; Steve M Gordon; Gary W Procop
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

10.  Molecular typing of a Legionella pneumophila outbreak in Ontario, Canada.

Authors:  Matthew W Gilmour; Kathryn Bernard; Dobryan M Tracz; Adam B Olson; Cindi R Corbett; Tamara Burdz; Betty Ng; Deborah Wiebe; George Broukhanski; Peter Boleszczuk; Patrick Tang; Frances Jamieson; Gary Van Domselaar; Francis A Plummer; Jody D Berry
Journal:  J Med Microbiol       Date:  2007-03       Impact factor: 2.472
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