Literature DB >> 12718919

Light scattering as a diagnostic for protein crystal growth--a practical approach.

W William Wilson1.   

Abstract

Static and dynamic light scattering are discussed as particularly useful tools for studying various aspects of protein crystal growth. Specific applications for prenucleation assays as well as for monitoring postnucleation growth processes are presented. Protein-protein interactions determined by light scattering, which serve as a predictor for favorable crystallization conditions as well as for protein solubility behavior, are detailed. Several precautions regarding the practical aspects of light scattering and interpretation of data are also discussed.

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Year:  2003        PMID: 12718919     DOI: 10.1016/s1047-8477(03)00038-8

Source DB:  PubMed          Journal:  J Struct Biol        ISSN: 1047-8477            Impact factor:   2.867


  39 in total

Review 1.  Protein crystallization in the structural genomics era.

Authors:  Alexander McPherson
Journal:  J Struct Funct Genomics       Date:  2004

2.  Biocrystallography: past, present, future.

Authors:  Richard Giegé; Claude Sauter
Journal:  HFSP J       Date:  2010-04-22

3.  Crystallization of hepatocyte nuclear factor 1beta in complex with DNA.

Authors:  Peng Lu; Yun Li; Amanda Gorman; Young-In Chi
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-05-05

4.  Structural genomics of eukaryotic targets at a laboratory scale.

Authors:  Didier Busso; Pierre Poussin-Courmontagne; David Rosé; Raymond Ripp; Alain Litt; Jean-Claude Thierry; Dino Moras
Journal:  J Struct Funct Genomics       Date:  2005

5.  Identifying protein construct variants with increased crystallization propensity--a case study.

Authors:  Guido A Malawski; Roman C Hillig; Felipe Monteclaro; Uwe Eberspaecher; Arndt A P Schmitz; Kerstin Crusius; Martina Huber; Ursula Egner; Peter Donner; Beate Müller-Tiemann
Journal:  Protein Sci       Date:  2006-12       Impact factor: 6.725

6.  Disruption of PF4/H multimolecular complex formation with a minimally anticoagulant heparin (ODSH).

Authors:  M V Joglekar; P M Quintana Diez; S Marcus; R Qi; B Espinasse; M R Wiesner; E Pempe; J Liu; D M Monroe; G M Arepally
Journal:  Thromb Haemost       Date:  2012-02-08       Impact factor: 5.249

Review 7.  The neurobiologist's guide to structural biology: a primer on why macromolecular structure matters and how to evaluate structural data.

Authors:  Daniel L Minor
Journal:  Neuron       Date:  2007-05-24       Impact factor: 17.173

8.  Crystallization Optimum Solubility Screening: using crystallization results to identify the optimal buffer for protein crystal formation.

Authors:  Bernard Collins; Raymond C Stevens; Rebecca Page
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-11-05

9.  Protein-protein interaction on lysozyme crystallization revealed by rotational diffusion analysis.

Authors:  Daisuke Takahashi; Etsuko Nishimoto; Tadashi Murase; Shoji Yamashita
Journal:  Biophys J       Date:  2008-02-29       Impact factor: 4.033

10.  Protein phase behavior in aqueous solutions: crystallization, liquid-liquid phase separation, gels, and aggregates.

Authors:  André C Dumetz; Aaron M Chockla; Eric W Kaler; Abraham M Lenhoff
Journal:  Biophys J       Date:  2008-01-15       Impact factor: 4.033

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