Literature DB >> 12654693

PCR-oligonucleotide ligation assay for detection of point mutations associated with quinolone resistance in Streptococcus pneumoniae.

Mai-Ha Bui1, Gregory G Stone, Angela M Nilius, Laurel Almer, Robert K Flamm.   

Abstract

We have developed a PCR-oligonucleotide ligation assay to rapidly identify base substitutions in topoisomerase genes that are associated with quinolone resistance in clinical isolates of Streptococcus pneumoniae. Thirty-seven strains for which the ciprofloxacin MICs were >/=4 micro g/ml and 16 strains for which the MICs were </=2 micro g/ml were assayed. Compared with sequence data, the assay correctly identified the DNA bases that encoded amino acids at the four positions most commonly associated with quinolone resistance (Ser79 and Asp83 of ParC and Ser81 and Glu85 of GyrA). Therefore, this procedure can rapidly distinguish single base substitutions associated with quinolone-resistant topoisomerases in S. pneumoniae.

Entities:  

Mesh：

Substances：

Year:  2003        PMID： 12654693      PMCID： PMC152482          DOI： 10.1128/AAC.47.4.1456-1459.2003

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  13 in total

1.  Prevalence of gyrA, gyrB, parC, and parE mutations in clinical isolates of Streptococcus pneumoniae with decreased susceptibilities to different fluoroquinolones and originating from Worldwide Surveillance Studies during the 1997-1998 respiratory season.

Authors:  M E Jones; D F Sahm; N Martin; S Scheuring; P Heisig; C Thornsberry; K Köhrer; F J Schmitz
Journal:  Antimicrob Agents Chemother       Date:  2000-02       Impact factor: 5.191

2.  Automated DNA diagnostics using an ELISA-based oligonucleotide ligation assay.

Authors:  D A Nickerson; R Kaiser; S Lappin; J Stewart; L Hood; U Landegren
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

3.  A PCR-oligonucleotide ligation assay to determine the prevalence of 23S rRNA gene mutations in clarithromycin-resistant Helicobacter pylori.

Authors:  G G Stone; D Shortridge; J Versalovic; J Beyer; R K Flamm; D Y Graham; A T Ghoneim; S K Tanaka
Journal:  Antimicrob Agents Chemother       Date:  1997-03       Impact factor: 5.191

4.  Apparent involvement of a multidrug transporter in the fluoroquinolone resistance of Streptococcus pneumoniae.

Authors:  N N Baranova; A A Neyfakh
Journal:  Antimicrob Agents Chemother       Date:  1997-06       Impact factor: 5.191

5.  ParC subunit of DNA topoisomerase IV of Streptococcus pneumoniae is a primary target of fluoroquinolones and cooperates with DNA gyrase A subunit in forming resistance phenotype.

Authors:  R Muñoz; A G De La Campa
Journal:  Antimicrob Agents Chemother       Date:  1996-10       Impact factor: 5.191

6.  Contribution of mutations in gyrA and parC genes to fluoroquinolone resistance of mutants of Streptococcus pneumoniae obtained in vivo and in vitro.

Authors:  J Tankovic; B Perichon; J Duval; P Courvalin
Journal:  Antimicrob Agents Chemother       Date:  1996-11       Impact factor: 5.191

7.  Activities of clinafloxacin, gatifloxacin, gemifloxacin, and trovafloxacin against recent clinical isolates of levofloxacin-resistant Streptococcus pneumoniae.

Authors:  J H Jorgensen; L M Weigel; J M Swenson; C G Whitney; M J Ferraro; F C Tenover
Journal:  Antimicrob Agents Chemother       Date:  2000-11       Impact factor: 5.191

8.  Fluoroquinolone resistance in clinical isolates of Streptococcus pneumoniae: contributions of type II topoisomerase mutations and efflux to levels of resistance.

Authors:  D J Bast; D E Low; C L Duncan; L Kilburn; L A Mandell; R J Davidson; J C de Azavedo
Journal:  Antimicrob Agents Chemother       Date:  2000-11       Impact factor: 5.191

9.  DNA gyrase and topoisomerase IV are dual targets of clinafloxacin action in Streptococcus pneumoniae.

Authors:  X S Pan; L M Fisher
Journal:  Antimicrob Agents Chemother       Date:  1998-11       Impact factor: 5.191

10.  Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay.

Authors:  L Karim; W Coppieters; L Grobet; A Valentini; M Georges
Journal:  Anim Genet       Date:  2000-12       Impact factor: 3.169
View more
  5 in total

1.  New real-time PCR assay using locked nucleic acid probes to assess prevalence of ParC mutations in fluoroquinolone-susceptible Streptococcus pneumoniae isolates from France.

Authors:  Jean-Winoc Decousser; Imen Methlouthi; Patrick Pina; Anne Collignon; Pierre Allouch
Journal:  Antimicrob Agents Chemother       Date:  2006-04       Impact factor: 5.191

2.  Rapid detection of a point mutation in the parC gene associated with decreased susceptibility to fluoroquinolones in Mycoplasma bovis.

Authors:  I Lysnyansky; I Mikula; I Gerchman; S Levisohn
Journal:  Antimicrob Agents Chemother       Date:  2009-08-31       Impact factor: 5.191

3.  Oligonucleotide ligation assay for detection of mutations associated with reverse transcriptase and protease inhibitor resistance in non-B subtypes and recombinant forms of human immunodeficiency virus type 1.

Authors:  Yolanda Vega; Lucía Pérez-Alvárez; Elena Delgado; Mercedes Muñoz; Gema Casado; Rocío Carmona; María Sierra; Elena Vázquez de Parga; Milagros Pinilla; Valentina García; Leandro Medrano; Gerardo Contreras; Miguel Thomson; Rafael Nájera
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

4.  Rapid screening of topoisomerase gene mutations by a novel melting curve analysis method for early warning of fluoroquinolone-resistant Streptococcus pneumoniae emergence.

Authors:  Kazuko Y Fukushima; Yoichi Hirakata; Kazuyuki Sugahara; Katsunori Yanagihara; Akira Kondo; Shigeru Kohno; Shimeru Kamihira
Journal:  J Clin Microbiol       Date:  2006-10-04       Impact factor: 5.948

5.  Point-of-Care Tests for HIV Drug Resistance Monitoring: Advances and Potentials.

Authors:  Rayeil J Chua; Rupert Capiña; Hezhao Ji
Journal:  Pathogens       Date:  2022-06-25
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.