Literature DB >> 14532229

Real-time PCR assay targets the 23S-5S spacer for direct detection and differentiation of Legionella spp. and Legionella pneumophila.

Bjorn L Herpers1, Bartelt M de Jongh, Kim van der Zwaluw, Erik J van Hannen.   

Abstract

A real-time PCR for the ABI Prism 7000 system targeting the 23S-5S spacer of Legionella spp. was developed. Simultaneous detection and differentiation of Legionella spp. and Legionella pneumophila within 90 min and without post-PCR melting-curve analysis was achieved using two TaqMan probes. In sputum samples from 23 controls and 17 patients with legionellosis, defined by positive culture, urinary antigen testing, or seroconversion, 94% sensitivity and 100% specificity were observed.

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Year:  2003        PMID： 14532229      PMCID： PMC254366          DOI： 10.1128/JCM.41.10.4815-4816.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  Detection of Legionella pneumophila antigen in urine samples by the BinaxNOW immunochromatographic assay and comparison with both Binax Legionella Urinary Enzyme Immunoassay (EIA) and Biotest Legionella Urin Antigen EIA.

Authors:  Jürgen H Helbig; Søren A Uldum; P Christian Lück; Timothy G Harrison
Journal:  J Med Microbiol       Date:  2001-06       Impact factor: 2.472

2.  Qualitative detection of Legionella species in bronchoalveolar lavages and induced sputa by automated DNA extraction and real-time polymerase chain reaction.

Authors:  R B Raggam; E Leitner; G Mühlbauer; J Berg; M Stöcher; A J Grisold; E Marth; H H Kessler
Journal:  Med Microbiol Immunol       Date:  2002-08-29       Impact factor: 3.402

3.  Development of conventional and real-time PCR assays for detection of Legionella DNA in respiratory specimens.

Authors:  K Rantakokko-Jalava; J Jalava
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

4.  A Legionella pneumophila gene encoding a species-specific surface protein potentiates initiation of intracellular infection.

Authors:  N P Cianciotto; B I Eisenstein; C H Mody; G B Toews; N C Engleberg
Journal:  Infect Immun       Date:  1989-04       Impact factor: 3.441

5.  Detection of Legionella species in respiratory specimens using PCR with sequencing confirmation.

Authors:  J L Cloud; K C Carroll; P Pixton; M Erali; D R Hillyard
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

6.  Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR.

Authors:  N Wellinghausen; C Frost; R Marre
Journal:  Appl Environ Microbiol       Date:  2001-09       Impact factor: 4.792

7.  Direct detection of Legionella species from bronchoalveolar lavage and open lung biopsy specimens: comparison of LightCycler PCR, in situ hybridization, direct fluorescence antigen detection, and culture.

Authors:  R T Hayden; J R Uhl; X Qian; M K Hopkins; M C Aubry; A H Limper; R V Lloyd; F R Cockerill
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

8.  Interspecies sequence differences in the Mip protein from the genus Legionella: implications for function and evolutionary relatedness.

Authors:  R M Ratcliff; S C Donnellan; J A Lanser; P A Manning; M W Heuzenroeder
Journal:  Mol Microbiol       Date:  1997-09       Impact factor: 3.501

Review 9.  Legionella and Legionnaires' disease: 25 years of investigation.

Authors:  Barry S Fields; Robert F Benson; Richard E Besser
Journal:  Clin Microbiol Rev       Date:  2002-07       Impact factor: 26.132

10.  Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-color real-time PCR and melting curve analysis.

Authors:  Udo Reischl; Hans-Jörg Linde; Norbert Lehn; Olfert Landt; Kevin Barratt; Nele Wellinghausen
Journal:  J Clin Microbiol       Date:  2002-10       Impact factor: 5.948
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  26 in total

1.  Multicenter comparison of molecular methods for detection of Legionella spp. in sputum samples.

Authors:  M A Bencini; A J C van den Brule; E C J Claas; M H A Hermans; W J G Melchers; G T Noordhoek; M M M Salimans; J Schirm; C Vink; A van der Zee; R Jansen
Journal:  J Clin Microbiol       Date:  2007-08-01       Impact factor: 5.948

2.  Quantitative detection of Legionella pneumophila in water samples by immunomagnetic purification and real-time PCR amplification of the dotA gene.

Authors:  M A Yáñez; C Carrasco-Serrano; V M Barberá; V Catalán
Journal:  Appl Environ Microbiol       Date:  2005-07       Impact factor: 4.792

Review 3.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

Review 4.  Current and emerging Legionella diagnostics for laboratory and outbreak investigations.

Authors:  Jeffrey W Mercante; Jonas M Winchell
Journal:  Clin Microbiol Rev       Date:  2015-01       Impact factor: 26.132

Review 5.  Diagnosis of Legionella infection in Legionnaires' disease.

Authors:  J W Den Boer; E P F Yzerman
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2004-12       Impact factor: 3.267

6.  Two-step scheme for rapid identification and differentiation of Legionella pneumophila and non-Legionella pneumophila species.

Authors:  Xiao-Yong Zhan; Lian-Qing Li; Chao-Hui Hu; Qing-Yi Zhu
Journal:  J Clin Microbiol       Date:  2009-12-09       Impact factor: 5.948

7.  Evaluation of the microbiological quality of reclaimed water produced from a lagooning system.

Authors:  X Fernandez-Cassi; C Silvera; S Cervero-Aragó; M Rusiñol; F Latif-Eugeni; C Bruguera-Casamada; S Civit; R M Araujo; M J Figueras; R Girones; S Bofill-Mas
Journal:  Environ Sci Pollut Res Int       Date:  2016-05-19       Impact factor: 4.223

8.  Identification and quantitative detection of Legionella spp. in various aquatic environments by real-time PCR assay.

Authors:  Po-Min Kao; Min-Che Tung; Bing-Mu Hsu; Yi-Chou Chiu; Cheng-Yu She; Shu-Min Shen; Yu-Li Huang; Wen-Chien Huang
Journal:  Environ Sci Pollut Res Int       Date:  2013-03-28       Impact factor: 4.223

Review 9.  Diagnostic Accuracy of PCR Alone and Compared to Urinary Antigen Testing for Detection of Legionella spp.: a Systematic Review.

Authors:  Tomer Avni; Amir Bieber; Hefziba Green; Tali Steinmetz; Leonard Leibovici; Mical Paul
Journal:  J Clin Microbiol       Date:  2015-12-09       Impact factor: 5.948

10.  Lipopolysaccharide-induced pro-inflammatory cytokines in the brain of rats in the morphine-tolerant state.

Authors:  Linda Staikos; Lorenc Malellari; Sulie L Chang
Journal:  J Neuroimmune Pharmacol       Date:  2008-06-27       Impact factor: 4.147
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