Literature DB >> 12351852

Automated mounting, centering and screening of crystals for high-throughput protein crystallography.

W I Karain1, G P Bourenkov, H Blume, H D Bartunik.   

Abstract

A fully automated system for screening protein crystals for X-ray diffraction analysis has been designed and is being installed on the beamline BW6 at DORIS in Hamburg, Germany. The system includes robotic mounting of flash-frozen crystals from a storage dewar, centering and alignment of the sample both by optical and X-ray (scattering and fluorescence) techniques, assessment of the diffraction quality of the sample, and SAD/MAD or non-conventional diffraction data acquisition with high-throughput data rates. The system covers all experimental steps required for protein x-ray structure analysis and provides a powerful means for structural genomics projects.

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Year:  2002        PMID： 12351852     DOI： 10.1107/s0907444902012751

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  21 in total

Review 1.  Protein crystallization in the structural genomics era.

Authors:  Alexander McPherson
Journal:  J Struct Funct Genomics       Date:  2004

Review 2.  Automated robotic harvesting of protein crystals-addressing a critical bottleneck or instrumentation overkill?

Authors:  Robert Viola; Peter Carman; Jace Walsh; Daniel Frankel; Bernhard Rupp
Journal:  J Struct Funct Genomics       Date:  2007-10-27

3.  Fast fluorescence techniques for crystallography beamlines.

Authors:  Sergey Stepanov; Mark Hilgart; Derek W Yoder; Oleg Makarov; Michael Becker; Ruslan Sanishvili; Craig M Ogata; Nagarajan Venugopalan; David Aragão; Martin Caffrey; Janet L Smith; Robert F Fischetti
Journal:  J Appl Crystallogr       Date:  2011-06-08       Impact factor: 3.304

4.  First experiences with semi-autonomous robotic harvesting of protein crystals.

Authors:  Robert Viola; Jace Walsh; Alex Melka; Wesley Womack; Sean Murphy; Alan Riboldi-Tunnicliffe; Bernhard Rupp
Journal:  J Struct Funct Genomics       Date:  2011-03-23

5.  Approaches to automated protein crystal harvesting.

Authors:  Marc C Deller; Bernhard Rupp
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2014-01-28       Impact factor: 1.056

Review 6.  Current advances in synchrotron radiation instrumentation for MX experiments.

Authors:  Robin L Owen; Jordi Juanhuix; Martin Fuchs
Journal:  Arch Biochem Biophys       Date:  2016-04-01       Impact factor: 4.013

Review 7.  Structure-based functional inference in structural genomics.

Authors:  Sung-Hou Kim; Dong Hae Shin; In-Geol Choi; Ursula Schulze-Gahmen; Shengfeng Chen; Rosalind Kim
Journal:  J Struct Funct Genomics       Date:  2003

8.  UV LED lighting for automated crystal centring.

Authors:  Leonard M G Chavas; Yusuke Yamada; Masahiko Hiraki; Noriyuki Igarashi; Naohiro Matsugaki; Soichi Wakatsuki
Journal:  J Synchrotron Radiat       Date:  2010-11-05       Impact factor: 2.616

9.  Integrated nonlinear optical imaging microscope for on-axis crystal detection and centering at a synchrotron beamline.

Authors:  Jeremy T Madden; Scott J Toth; Christopher M Dettmar; Justin A Newman; Robert A Oglesbee; Hartmut G Hedderich; R Michael Everly; Michael Becker; Judith A Ronau; Susan K Buchanan; Vadim Cherezov; Marie E Morrow; Shenglan Xu; Dale Ferguson; Oleg Makarov; Chittaranjan Das; Robert Fischetti; Garth J Simpson
Journal:  J Synchrotron Radiat       Date:  2013-05-03       Impact factor: 2.616

10.  Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source.

Authors:  Scott Classen; Greg L Hura; James M Holton; Robert P Rambo; Ivan Rodic; Patrick J McGuire; Kevin Dyer; Michal Hammel; George Meigs; Kenneth A Frankel; John A Tainer
Journal:  J Appl Crystallogr       Date:  2013-01-17       Impact factor: 3.304
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