Literature DB >> 12089301

Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system.

Nabin K Shrestha1, Marion J Tuohy, Gerri S Hall, Carlos M Isada, Gary W Procop.   

Abstract

One hundred BacT/ALERT blood culture bottles growing gram-positive cocci in clusters were cultured and studied by LightCycler PCR for the sa442 and mecA genes. PCR was 100% sensitive and specific for detecting Staphylococcus aureus and methicillin resistance in S. aureus but was less accurate for methicillin resistance in coagulase-negative staphylococci.

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Year:  2002        PMID： 12089301      PMCID： PMC120611          DOI： 10.1128/JCM.40.7.2659-2661.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  Correlation between genotype and phenotypic categorization of staphylococci based on methicillin susceptibility and resistance.

Authors:  E Gradelski; L Valera; L Aleksunes; D Bonner; J Fung-Tomc
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

2.  Rapid identification of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation using real-time fluorescence PCR.

Authors:  U Reischl; H J Linde; M Metz; B Leppmeier; N Lehn
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

3.  Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis.

Authors:  C Ryffel; W Tesch; I Birch-Machin; P E Reynolds; L Barberis-Maino; F H Kayser; B Berger-Bächi
Journal:  Gene       Date:  1990-09-28       Impact factor: 3.688

4.  Direct detection of mecA, nuc and 16S rRNA genes in BacT/Alert blood culture bottles.

Authors:  P Lem; J Spiegelman; B Toye; K Ramotar
Journal:  Diagn Microbiol Infect Dis       Date:  2001-11       Impact factor: 2.803

5.  Rapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR.

Authors:  T Y Tan; S Corden; R Barnes; B Cookson
Journal:  J Clin Microbiol       Date:  2001-12       Impact factor: 5.948

6.  Homology of mecA gene in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans to that of Staphylococcus aureus.

Authors:  K Ubukata; R Nonoguchi; M D Song; M Matsuhashi; M Konno
Journal:  Antimicrob Agents Chemother       Date:  1990-01       Impact factor: 5.191

7.  Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus.

Authors:  F Martineau; F J Picard; P H Roy; M Ouellette; M G Bergeron
Journal:  J Clin Microbiol       Date:  1998-03       Impact factor: 5.948
  7 in total
  43 in total

1.  Rapid identification of Staphylococcus aureus from BacT/ALERT blood culture bottles by direct Gram stain characteristics.

Authors:  D R Murdoch; R L Greenlees
Journal:  J Clin Pathol       Date:  2004-02       Impact factor: 3.411

2.  Clinical Staphylococcus aureus isolate negative for the Sa442 fragment.

Authors:  Corné H W Klaassen; Hanneke A de Valk; Alphons M Horrevorts
Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948

3.  Detection of methicillin-resistant Staphylococcus aureus (MRSA) in blood with the EVIGENE MRSA detection kit.

Authors:  Katrina Levi; Kevin J Towner
Journal:  J Clin Microbiol       Date:  2003-08       Impact factor: 5.948

4.  Direct susceptibility testing of positive blood cultures by using Sensititre broth microdilution plates.

Authors:  Kimberle C Chapin; Michael C Musgnug
Journal:  J Clin Microbiol       Date:  2003-10       Impact factor: 5.948

5.  One-step sample preparation of positive blood cultures for the direct detection of methicillin-sensitive and -resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci within one hour using the automated GenomEra CDX™ PCR system.

Authors:  J J Hirvonen; P von Lode; M Nevalainen; K Rantakokko-Jalava; S-S Kaukoranta
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2012-05-26       Impact factor: 3.267

6.  False-negative test results in the Slidex Staph Plus (bioMérieux) agglutination test are mainly caused by spa-type t001 and t001-related strains.

Authors:  F Szabados; J Woloszyn; M Kaase; S G Gatermann
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2010-10-09       Impact factor: 3.267

7.  Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay.

Authors:  David K Warren; Robert S Liao; Liana R Merz; Michael Eveland; W Michael Dunne
Journal:  J Clin Microbiol       Date:  2004-12       Impact factor: 5.948

8.  Shortcut method for extraction of Staphylococcus aureus DNA from blood cultures and conventional cultures for use in real-time PCR assays.

Authors:  D Nicholas Adams
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

Review 9.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

10.  Evaluation of an isothermal signal amplification method for rapid detection of methicillin-resistant Staphylococcus aureus from patient-screening swabs.

Authors:  Katrina Levi; Claire Bailey; Alexandra Bennett; Peter Marsh; Don L N Cardy; Kevin J Towner
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948
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