Literature DB >> 12904411

Detection of methicillin-resistant Staphylococcus aureus (MRSA) in blood with the EVIGENE MRSA detection kit.

Katrina Levi1, Kevin J Towner.   

Abstract

A total of 200 blood cultures containing putative staphylococci were analyzed by a commercial gene probe hybridization assay (EVIGENE; Statens Serum Institut, Copenhagen, Denmark), and 18 were identified as methicillin-resistant Staphylococcus aureus (MRSA) positive. Of these, 17 were positive by PCR and 16 were positive by culture. Detailed analysis of the discrepant results showed that the EVIGENE kit allowed specific identification of MRSA in blood cultures without any of the drawbacks associated with PCR.

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Year:  2003        PMID： 12904411      PMCID： PMC179827          DOI： 10.1128/JCM.41.8.3890-3892.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

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Authors:  V A Kempf; K Trebesius; I B Autenrieth
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative Staphylococci and determine methicillin resistance from blood cultures.

Authors:  N Maes; J Magdalena; S Rottiers; Y De Gheldre; M J Struelens
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

3.  Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs.

Authors:  D Jonas; M Speck; F D Daschner; H Grundmann
Journal:  J Clin Microbiol       Date:  2002-05       Impact factor: 5.948

4.  Rapid detection of methicillin-resistant staphylococci from blood culture bottles by using a multiplex PCR assay.

Authors:  L Louie; J Goodfellow; P Mathieu; A Glatt; M Louie; A E Simor
Journal:  J Clin Microbiol       Date:  2002-08       Impact factor: 5.948

5.  Direct detection of mecA, nuc and 16S rRNA genes in BacT/Alert blood culture bottles.

Authors:  P Lem; J Spiegelman; B Toye; K Ramotar
Journal:  Diagn Microbiol Infect Dis       Date:  2001-11       Impact factor: 2.803

6.  Evaluation of a new 3-h hybridization method for detecting the mecA gene in Staphylococcus aureus and comparison with existing genotypic and phenotypic susceptibility testing methods.

Authors:  R L Skov; L V Pallesen; R L Poulsen; F Espersen
Journal:  J Antimicrob Chemother       Date:  1999-04       Impact factor: 5.790

7.  Rapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR.

Authors:  T Y Tan; S Corden; R Barnes; B Cookson
Journal:  J Clin Microbiol       Date:  2001-12       Impact factor: 5.948

8.  Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes.

Authors:  Kenneth Oliveira; Gary W Procop; Deborah Wilson; James Coull; Henrik Stender
Journal:  J Clin Microbiol       Date:  2002-01       Impact factor: 5.948

9.  Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis.

Authors:  Sara E Cosgrove; George Sakoulas; Eli N Perencevich; Mitchell J Schwaber; Adolf W Karchmer; Yehuda Carmeli
Journal:  Clin Infect Dis       Date:  2002-12-13       Impact factor: 9.079

10.  Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system.

Authors:  Nabin K Shrestha; Marion J Tuohy; Gerri S Hall; Carlos M Isada; Gary W Procop
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948
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  7 in total

1.  A probe-based method for confirmation of methicillin-resistant Staphylococcus aureus and detection of Panton-Valentine leukocidin and tst virulence genes.

Authors:  Ashok Srinivasan; Matthew J Bankowski; Steven E Seifried; Sadao Jinno; Rosalie Perkins; Seema Singh; Claire Ying; Alan D Tice; Wesley Kim; Randall T Hayden
Journal:  Diagn Microbiol Infect Dis       Date:  2011-06-11       Impact factor: 2.803

2.  Rapid identification of staphylococcal strains from positive-testing blood culture bottles by internal transcribed spacer PCR followed by microchip gel electrophoresis.

Authors:  Shin-Ichi Fujita; Yasuko Senda; Thikako Iwagami; Takuma Hashimoto
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948

Review 3.  Rapid diagnostics for methicillin-resistant Staphylococcus aureus: current status.

Authors:  Karen C Carroll
Journal:  Mol Diagn Ther       Date:  2008       Impact factor: 4.074

4.  Rapid Detection of Methicillin-Resistant Staphylococcus aureus and Methicillin-Susceptible S. aureus Directly from Positive Blood Cultures by Use of the BD Max StaphSR Assay.

Authors:  Justin A Ellem; Tom Olma; Matthew V N O'Sullivan
Journal:  J Clin Microbiol       Date:  2015-12       Impact factor: 5.948

5.  Clinical validation of the molecular BD GeneOhm StaphSR assay for direct detection of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in positive blood cultures.

Authors:  Paul D Stamper; Mian Cai; Tracy Howard; Sharon Speser; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2007-05-23       Impact factor: 5.948

6.  A study of the efficacy and cost-effectiveness of MRSA screening and monitoring on surgical wards using a new, rapid molecular test (EMMS).

Authors:  Katherine J Hardy; Ala Szczepura; Ruth Davies; Andrew Bradbury; Nigel Stallard; Savita Gossain; Paul Walley; Peter M Hawkey
Journal:  BMC Health Serv Res       Date:  2007-10-03       Impact factor: 2.655

7.  A new restriction endonuclease-based method for highly-specific detection of DNA targets from methicillin-resistant Staphylococcus aureus.

Authors:  Maria W Smith; Andrei L Ghindilis; Ihab A Seoudi; Kenneth Smith; Rosalind Billharz; Holly M Simon
Journal:  PLoS One       Date:  2014-05-15       Impact factor: 3.240
  7 in total

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