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Literature DB >> 15956423

Shortcut method for extraction of Staphylococcus aureus DNA from blood cultures and conventional cultures for use in real-time PCR assays.

Abstract

PCR allows rapid determination of both the presence of Staphylococcus aureus and of methicillin resistance. I present here a simple method for direct extraction from blood cultures or colonies for use in real-time PCR, eliminating tedious, time-consuming methods of DNA extraction which may otherwise deter microbiology labs from offering PCR.

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Year: 2005 PMID： 15956423 PMCID： PMC1151874 DOI： 10.1128/JCM.43.6.2932-2933.2005

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

15 in total

1. Purification and characterization of PCR-inhibitory components in blood cells.

Authors: W A Al-Soud; P Rådström
Journal: J Clin Microbiol Date: 2001-02 Impact factor: 5.948

2. Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative Staphylococci and determine methicillin resistance from blood cultures.

Authors: N Maes; J Magdalena; S Rottiers; Y De Gheldre; M J Struelens
Journal: J Clin Microbiol Date: 2002-04 Impact factor: 5.948

3. Detection of methicillin resistance in primary blood culture isolates of coagulase-negative staphylococci by PCR, slide agglutination, disk diffusion, and a commercial method.

Authors: Zafar Hussain; Luba Stoakes; Michael A John; Shaunalee Garrow; Viivi Fitzgerald
Journal: J Clin Microbiol Date: 2002-06 Impact factor: 5.948

4. Direct detection of mecA, nuc and 16S rRNA genes in BacT/Alert blood culture bottles.

Authors: P Lem; J Spiegelman; B Toye; K Ramotar
Journal: Diagn Microbiol Infect Dis Date: 2001-11 Impact factor: 2.803

5. Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR.

Authors: W A Al-Soud; L J Jönsson; P Râdström
Journal: J Clin Microbiol Date: 2000-01 Impact factor: 5.948

6. Rapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR.

Authors: T Y Tan; S Corden; R Barnes; B Cookson
Journal: J Clin Microbiol Date: 2001-12 Impact factor: 5.948

7. Improved amplification of microbial DNA from blood cultures by removal of the PCR inhibitor sodium polyanetholesulfonate.

Authors: D N Fredricks; D A Relman
Journal: J Clin Microbiol Date: 1998-10 Impact factor: 5.948

8. Rapid extraction from and direct identification in clinical samples of methicillin-resistant staphylococci using the PCR.

Authors: R I Jaffe; J D Lane; S V Albury; D M Niemeyer
Journal: J Clin Microbiol Date: 2000-09 Impact factor: 5.948

9. Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system.

Authors: Nabin K Shrestha; Marion J Tuohy; Gerri S Hall; Carlos M Isada; Gary W Procop
Journal: J Clin Microbiol Date: 2002-07 Impact factor: 5.948

10. Detection of methicillin-resistant Staphylococcus aureus and simultaneous confirmation by automated nucleic acid extraction and real-time PCR.

Authors: Andrea J Grisold; Eva Leitner; Gerhard Mühlbauer; Egon Marth; Harald H Kessler
Journal: J Clin Microbiol Date: 2002-07 Impact factor: 5.948

4 in total

1. Accuracy and potential usefulness of triplex real-time PCR for improving antibiotic treatment of patients with blood cultures showing clustered gram-positive cocci on direct smears.

Authors: Raymond Ruimy; Marie Dos-Santos; Laurent Raskine; Frédéric Bert; René Masson; Sandrine Elbaz; Christine Bonnal; Jean-Christophe Lucet; Agnès Lefort; Bruno Fantin; Michel Wolff; Michele Hornstein; Antoine Andremont
Journal: J Clin Microbiol Date: 2008-04-16 Impact factor: 5.948