| Literature DB >> 1141690 |
Abstract
The automated continuous flow system for the extraction and fluorometric analysis of histamine has been further improved. Samples with or without protein can be analyzed. The use of a single pump, added nitrogen, and lower concentration of o-phthalaldehyde increase the sensitivity by 5-10 fold and allow the analysis of samples at the rate of 30 per hour. Samples which are less than 0.5 ml in volume and contain 0.1 to 10 ng/ml histamine can routinely be analyzed. The sensitivity of the system is now 0.05 ng and compares favorably with isotopic techniques.Entities:
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Year: 1975 PMID： 1141690 DOI： 10.1016/0022-1759(75)90025-3
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303