Literature DB >> 10909865

A set of HNCO-based experiments for measurement of residual dipolar couplings in 15N, 13C, (2H)-labeled proteins.

P Permi1, P R Rosevear, A Annila.   

Abstract

Several HNCO-based three-dimensional experiments are described for the measurement of 13C'(i - 1)-13Calpha(i - 1), 5N(i)-13C'(i - 1), 15N(i)-13Calpha(i), 15N(i)-13Calpha(i - 1), 1H(N)(i)-13Calpha(i), 1H(N)(i)-13Calpha(i - 1), and 13Calpha(i - 1)-13Cbeta(i - 1) scalar and dipolar couplings in 15N, 13C, (2H)-labelled protein samples. These pulse sequences produce spin-state edited spectra superficially resembling an HNCO correlation spectrum, allowing accurate and simple measurement of couplings without introducing additional spectral crowding. Scalar and dipolar couplings are measured with good sensitivity from relatively large proteins, as demonstrated with three proteins: cardiac Troponin C, calerythrin and ubiquitin. Measurement of several dipolar couplings between spin-1/2 nuclei using spin-state selective 3D HNCO spectra provides a wealth of structural information.

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Year:  2000        PMID： 10909865     DOI： 10.1023/a:1008372624615

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  26 in total

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2.  Solution structure of cyanovirin-N, a potent HIV-inactivating protein.

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3.  Integration of spin-state-selective excitation into 2D NMR correlation experiments with the heteronuclear ZQ/2Q pi rotations for 1JXH- resolved E.COSY-type measurements of heteronuclear coupling constants in proteins.

Authors:  A Meissner; J O Duus; O W Sørensen
Journal:  J Biomol NMR       Date:  1997-07       Impact factor: 2.835

4.  High-resolution heteronuclear NMR of human ubiquitin in an aqueous liquid crystalline medium.

Authors:  A Bax; N Tjandra
Journal:  J Biomol NMR       Date:  1997-10       Impact factor: 2.835

Review 5.  Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra.

Authors:  M Ottiger; F Delaglio; A Bax
Journal:  J Magn Reson       Date:  1998-04       Impact factor: 2.229

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Authors:  J R Tolman; J M Flanagan; M A Kennedy; J H Prestegard
Journal:  Nat Struct Biol       Date:  1997-04

7.  Short selective pulses for biochemical applications.

Authors:  E Kupce; J Boyd; I D Campbell
Journal:  J Magn Reson B       Date:  1995-03

8.  Improved sensitivity and coherence selection for [15N,1H]-TROSY elements in triple resonance experiments.

Authors:  M Salzmann; G Wider; K Pervushin; K Wüthrich
Journal:  J Biomol NMR       Date:  1999-10       Impact factor: 2.835

9.  Measurement of 15N-13C J couplings in staphylococcal nuclease.

Authors:  F Delaglio; D A Torchia; A Bax
Journal:  J Biomol NMR       Date:  1991-11       Impact factor: 2.835

10.  Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions.

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  39 in total

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Authors:  J J Chou; S Gaemers; B Howder; J M Louis; A Bax
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2.  A new approach for obtaining sequential assignment of large proteins.

Authors:  P Permi; A Annila
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Review 3.  Radial sampling for fast NMR: Concepts and practices over three decades.

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4.  Protein loop closure using orientational restraints from NMR data.

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Journal:  Proteins       Date:  2011-12-13

5.  Angular dependence of 1J(Ni,Calphai) and 2J(Ni,Calpha(i-1)) coupling constants measured in J-modulated HSQCs.

Authors:  Julia Wirmer; Harald Schwalbe
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6.  Simultaneous measurement of protein one-bond and two-bond nitrogen-carbon coupling constants using an internally referenced quantitative J-correlated [(15)N,(1)H]-TROSY-HNC experiment.

Authors:  Hans L J Wienk; Mitcheell M Martínez; Gary N Yalloway; Jürgen M Schmidt; Carlos Pérez; Heinz Rüterjans; Frank Löhr
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7.  Quaternary structure built from subunits combining NMR and small-angle x-ray scattering data.

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8.  Alignment of chain-like molecules.

Authors:  Martti Louhivuori; Kai Fredriksson; Kimmo Pääkkönen; Perttu Permi; Arto Annila
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9.  A Bayesian approach for determining protein side-chain rotamer conformations using unassigned NOE data.

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10.  Measurement of residual dipolar couplings from 1Halpha to 13Calpha and 15N using a simple HNCA-based experiment.

Authors:  Perttu Permi
Journal:  J Biomol NMR       Date:  2003-12       Impact factor: 2.835
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