Literature DB >> 10835020

Reproducibility problems with the Abbott laboratories LCx assay for Chlamydia trachomatis and Neisseria gonorrhoeae.

A M Gronowski1, S Copper, D Baorto, P R Murray.   

Abstract

This study demonstrates that significant reproducibility problems can occur during routine use of the Abbott Laboratories LCx assay for Chlamydia trachomatis and Neisseria gonorrhoeae. These problems can go undetected by the quality control procedures outlined in the manufacturer's package insert. We outline here procedures for detecting and preventing contamination and reproducibility problems.

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Year:  2000        PMID： 10835020      PMCID： PMC86828          DOI： 10.1128/JCM.38.6.2416-2418.2000

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Reproducibility of the Roche Amplicor polymerase chain reaction assay for detection of infection by Chlamydia trachomatis in endocervical specimens.

Authors:  G M Mulcahy; E A Albanese; B L Bachl
Journal:  Clin Chem       Date:  1998-07       Impact factor: 8.327

2.  False-negative results of a ligase chain reaction assay to detect Chlamydia trachomatis due to inhibitors in urine.

Authors:  E S Berg; G Anestad; H Moi; G Størvold; K Skaug
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1997-10       Impact factor: 3.267

3.  Comparison of ligase chain reaction and culture for detection of Neisseria gonorrhoeae in genital and extragenital specimens.

Authors:  A Stary; S F Ching; L Teodorowicz; H Lee
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

4.  Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by ligase chain reaction-based assays with clinical specimens from various sites: implications for diagnostic testing and screening.

Authors:  M Buimer; G J van Doornum; S Ching; P G Peerbooms; P K Plier; D Ram; H H Lee
Journal:  J Clin Microbiol       Date:  1996-10       Impact factor: 5.948

5.  Reproducibility problems with the AMPLICOR PCR Chlamydia trachomatis test.

Authors:  E M Peterson; V Darrow; J Blanding; S Aarnaes; L M de la Maza
Journal:  J Clin Microbiol       Date:  1997-04       Impact factor: 5.948

6.  Urinary inhibitors of polymerase chain reaction and ligase chain reaction and testing of multiple specimens may contribute to lower assay sensitivities for diagnosing Chlamydia trachomatis infected women.

Authors:  M A Chernesky; D Jang; J Sellors; K Luinstra; S Chong; S Castriciano; J B Mahony
Journal:  Mol Cell Probes       Date:  1997-08       Impact factor: 2.365

7.  Comparison of performances of two commercially available tests, a PCR assay and a ligase chain reaction test, in detection of urogenital Chlamydia trachomatis infection.

Authors:  M Puolakkainen; E Hiltunen-Back; T Reunala; S Suhonen; P Lähteenmäki; M Lehtinen; J Paavonen
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

8.  Urine specimens from pregnant and nonpregnant women inhibitory to amplification of Chlamydia trachomatis nucleic acid by PCR, ligase chain reaction, and transcription-mediated amplification: identification of urinary substances associated with inhibition and removal of inhibitory activity.

Authors:  J Mahony; S Chong; D Jang; K Luinstra; M Faught; D Dalby; J Sellors; M Chernesky
Journal:  J Clin Microbiol       Date:  1998-11       Impact factor: 5.948

9.  Evaluation of the abbott LCx assay for detection of Neisseria gonorrhoeae in endocervical swab specimens from females.

Authors:  S C Kehl; K Georgakas; G R Swain; G Sedmak; S Gradus; A Singh; S Foldy
Journal:  J Clin Microbiol       Date:  1998-12       Impact factor: 5.948
  9 in total
  14 in total

1.  Current Issues in Screening for Chlamydia trachomatis.

Authors:  Robert L. Cook; Lars ØStergaard
Journal:  Curr Infect Dis Rep       Date:  2003-04       Impact factor: 3.725

2.  Confirming positive results of nucleic acid amplification tests (NAATs) for Chlamydia trachomatis: all NAATs are not created equal.

Authors:  J Schachter; E W Hook; D H Martin; D Willis; P Fine; D Fuller; J Jordan; W M Janda; M Chernesky
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948

3.  Evaluation of an automated liquid-handling system (Tecan Genesis RSP 100) in the Abbott LCx assay for Chlamydia trachomatis.

Authors:  K L Hanson; C P Cartwright
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

4.  Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by enzyme immunoassay, culture, and three nucleic acid amplification tests.

Authors:  E Van Dyck; M Ieven; S Pattyn; L Van Damme; M Laga
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

5.  The laboratory diagnosis of Neisseria gonorrhoeae.

Authors:  Lai-King Ng; Irene E Martin
Journal:  Can J Infect Dis Med Microbiol       Date:  2005-01       Impact factor: 2.471

6.  Multicenter evaluation of the BDProbeTec ET System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs.

Authors:  B Van Der Pol; D V Ferrero; L Buck-Barrington; E Hook; C Lenderman; T Quinn; C A Gaydos; J Lovchik; J Schachter; J Moncada; G Hall; M J Tuohy; R B Jones
Journal:  J Clin Microbiol       Date:  2001-03       Impact factor: 5.948

7.  Detection of Chlamydia trachomatis by nucleic acid amplification testing: our evaluation suggests that CDC-recommended approaches for confirmatory testing are ill-advised.

Authors:  Julius Schachter; Joan M Chow; Holly Howard; Gail Bolan; Jeanne Moncada
Journal:  J Clin Microbiol       Date:  2006-07       Impact factor: 5.948

8.  External quality assessment for detection of Chlamydia trachomatis.

Authors:  V J Chalker; H Vaughan; P Patel; A Rossouw; H Seyedzadeh; K Gerrard; V L A James
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948

9.  Accuracy of results obtained by performing a second ligase chain reaction assay and PCR analysis on urine samples with positive or near-cutoff results in the LCx test for Chlamydia trachomatis.

Authors:  S Castriciano; K Luinstra; D Jang; J Patel; J Mahony; J Kapala; M Chernesky
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

10.  Evaluation of Gen-Probe APTIMA-based Neisseria gonorrhoeae and Chlamydia trachomatis confirmatory testing in a metropolitan setting of high disease prevalence.

Authors:  Erik Munson; Vivian Boyd; Jolanta Czarnecka; Judy Griep; Brian Lund; Nancy Schaal; Jeanne E Hryciuk
Journal:  J Clin Microbiol       Date:  2007-06-20       Impact factor: 5.948
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