Literature DB >> 10799469

Diagnosis of Enterocytozoon bieneusi by PCR in stool samples eluted from filter paper disks.

S Carnevale1, J N Velásquez, J H Labbé, A Chertcoff, M G Cabrera, M I Rodríguez.   

Abstract

We report a PCR-based assay for the detection of Enterocytozoon bieneusi. We extracted DNA from feces which had been applied to filter paper disks and evaluated four preserving solutions. Infected specimens were identified by electrophoresis of amplicons from concentrated formalin-fixed samples and unconcentrated fresh feces. Our findings demonstrate that this methodology is effective for sample collection, mailing, and diagnosis of this pathogen.

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Year:  2000        PMID： 10799469      PMCID： PMC95902          DOI： 10.1128/CDLI.7.3.504-506.2000

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  42 in total

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Journal:  Arch Pathol Lab Med       Date:  1997-08       Impact factor: 5.534

2.  DNA elution and amplification by polymerase chain reaction from dried blood spots.

Authors:  C Carducci; L Ellul; I Antonozzi; A Pontecorvi
Journal:  Biotechniques       Date:  1992-11       Impact factor: 1.993

3.  Characteristics of the microsporidian Enterocytozoon bieneusi: a consequence of its development within short-living enterocytes.

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Journal:  J Protozool       Date:  1991 Nov-Dec

4.  Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA.

Authors:  A J da Silva; D A Schwartz; G S Visvesvara; H de Moura; S B Slemenda; N J Pieniazek
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948

5.  Fluorescence techniques for diagnosing intestinal microsporidiosis in stool, enteric fluid, and biopsy specimens from acquired immunodeficiency syndrome patients with chronic diarrhea.

Authors:  C N Conteas; T Sowerby; G W Berlin; F Dahlan; A Nguyen; R Porschen; J Donovan; M LaRiviere; J M Orenstein
Journal:  Arch Pathol Lab Med       Date:  1996-09       Impact factor: 5.534

6.  DNA microextraction from dried blood spots on filter paper blotters: potential applications to newborn screening.

Authors:  E R McCabe; S Z Huang; W K Seltzer; M L Law
Journal:  Hum Genet       Date:  1987-03       Impact factor: 4.132

7.  Comparison of polymerase chain reaction with light microscopy for detection of microsporidia in clinical specimens.

Authors:  S Katzwinkel-Wladarsch; P Deplazes; R Weber; T Löscher; H Rinder
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8.  Detection of the microsporidian parasite Enterocytozoon bieneusi in specimens from patients with AIDS by PCR.

Authors:  J N Velásquez; S Carnevale; E A Guarnera; J H Labbé; A Chertcoff; M G Cabrera; M I Rodríguez
Journal:  J Clin Microbiol       Date:  1996-12       Impact factor: 5.948

9.  Identification of microsporidia in stool specimens by using PCR and restriction endonucleases.

Authors:  D P Fedorko; N A Nelson; C P Cartwright
Journal:  J Clin Microbiol       Date:  1995-07       Impact factor: 5.948

10.  Small subunit rRNA sequence of Enterocytozoon bieneusi and its potential diagnostic role with use of the polymerase chain reaction.

Authors:  X Zhu; M Wittner; H B Tanowitz; D Kotler; A Cali; L M Weiss
Journal:  J Infect Dis       Date:  1993-12       Impact factor: 5.226
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  8 in total

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Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

2.  Zinc PVA versus potassium dichromate for preservation of microsporidian spores of human origin.

Authors:  Hend Aly El-Taweel; Mona Mohammed Tolba; Hayam Abdelmonem Sadaka; Lobna Abdelaziz El-Zawawy; Mervat Mostafa Osman
Journal:  Parasitol Res       Date:  2012-03-20       Impact factor: 2.289

3.  Is "dried stool spots on filter paper method (DSSFP)" more sensitive and effective for detecting Blastocystis spp. and their subtypes by PCR and sequencing?

Authors:  Ayse Seyer; Djursun Karasartova; Emrah Ruh; Ayse Semra Güreser; Turgut Imir; Aysegul Taylan-Ozkan
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4.  Rapid extraction of DNA From Escherichia coli and Cryptosporidium parvum for use in PCR.

Authors:  J A Higgins; M C Jenkins; D R Shelton; R Fayer; J S Karns
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5.  PCR-based assay using occult blood detection cards for detection of diarrheagenic Escherichia coli in specimens from U.S. travelers to Mexico with acute diarrhea.

Authors:  Kevin A Grimes; Jamal A Mohamed; Herbert L Dupont; Ranjit S Padda; Zhi-Dong Jiang; Jose Flores; Jaime Belkind-Gerson; Francisco G Martinez-Sandoval; Pablo C Okhuysen
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6.  Chromatography paper strip sampling of enteric adenoviruses type 40 and 41 positive stool specimens.

Authors:  Kalina T Zlateva; Piet Maes; Mustafizur Rahman; Marc Van Ranst
Journal:  Virol J       Date:  2005-02-10       Impact factor: 4.099

7.  Diagnosis of amphimeriasis by LAMPhimerus assay in human stool samples long-term storage onto filter paper.

Authors:  William Cevallos; Pedro Fernández-Soto; Manuel Calvopiña; María Buendía-Sánchez; Julio López-Abán; Belén Vicente; Antonio Muro
Journal:  PLoS One       Date:  2018-02-14       Impact factor: 3.240

8.  Use of Occult Blood Detection Cards for Real-Time PCR-Based Diagnosis of Schistosoma Mansoni Infection.

Authors:  Mirjam Schunk; Seleshi Kebede Mekonnen; Beyene Wondafrash; Carolin Mengele; Erna Fleischmann; Karl-Heinz Herbinger; Jaco J Verweij; Christof Geldmacher; Gisela Bretzel; Thomas Löscher; Ahmed Zeynudin
Journal:  PLoS One       Date:  2015-09-11       Impact factor: 3.240
  8 in total

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