Literature DB >> 8815125

Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA.

A J da Silva1, D A Schwartz, G S Visvesvara, H de Moura, S B Slemenda, N J Pieniazek.   

Abstract

Enterocytozoon bieneusi is the most common microsporidian infecting patients with AIDS. We have developed a PCR primer pair, named EBIEF1/EBIER1, based on the small-subunit rRNA sequence of this microsporidian. Compared with other PCR-based methods, this primer pair shows a higher efficiency of detection in diagnostic applications than does another previously described primer pair, V1/EB450.

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Year:  1996        PMID： 8815125      PMCID： PMC228934          DOI： 10.1128/jcm.34.4.986-987.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

1.  Diagnosis of Enterocytozoon bieneusi microsporidiosis in AIDS patients by recovery of spores from faeces.

Authors:  T van Gool; W S Hollister; W E Schattenkerk; M A Van den Bergh Weerman; W J Terpstra; R J van Ketel; P Reiss; E U Canning
Journal:  Lancet       Date:  1990-09-15       Impact factor: 79.321

2.  Diagnosis of intestinal microsporidiosis by examination of stool and duodenal aspirate with Weber's modified trichrome and Uvitex 2B strains.

Authors:  P C DeGirolami; C R Ezratty; G Desai; A McCullough; D Asmuth; C Wanke; M Federman
Journal:  J Clin Microbiol       Date:  1995-04       Impact factor: 5.948

3.  Genetic evidence for the occurrence of extra-intestinal Enterocytozoon bieneusi infections.

Authors:  R A Hartskeerl; A R Schuitema; T van Gool; W J Terpstra
Journal:  Nucleic Acids Res       Date:  1993-08-25       Impact factor: 16.971

4.  Improved light-microscopical detection of microsporidia spores in stool and duodenal aspirates. The Enteric Opportunistic Infections Working Group.

Authors:  R Weber; R T Bryan; R L Owen; C M Wilcox; L Gorelkin; G S Visvesvara
Journal:  N Engl J Med       Date:  1992-01-16       Impact factor: 91.245

Review 5.  Microsporidiosis in HIV positive patients: current methods for diagnosis using biopsy, cytologic, ultrastructural, immunological, and tissue culture techniques.

Authors:  D A Schwartz; R T Bryan; R Weber; G S Visvesvara
Journal:  Folia Parasitol (Praha)       Date:  1994       Impact factor: 2.122

6.  Short-term in vitro culture and molecular analysis of the microsporidian, Enterocytozoon bieneusi.

Authors:  G Visvesvara; G J Leitch; N J Pieniazek; A J Da Silva; S Wallace; S B Slemenda; R Weber; D A Schwartz; L Gorelkin; C M Wilcox
Journal:  J Eukaryot Microbiol       Date:  1995 Sep-Oct       Impact factor: 3.346

Review 7.  Human microsporidial infections.

Authors:  R Weber; R T Bryan; D A Schwartz; R L Owen
Journal:  Clin Microbiol Rev       Date:  1994-10       Impact factor: 26.132

8.  Identification of microsporidia in stool specimens by using PCR and restriction endonucleases.

Authors:  D P Fedorko; N A Nelson; C P Cartwright
Journal:  J Clin Microbiol       Date:  1995-07       Impact factor: 5.948

9.  Small subunit rRNA sequence of Enterocytozoon bieneusi and its potential diagnostic role with use of the polymerase chain reaction.

Authors:  X Zhu; M Wittner; H B Tanowitz; D Kotler; A Cali; L M Weiss
Journal:  J Infect Dis       Date:  1993-12       Impact factor: 5.226

10.  Detection of microsporidia (Enterocytozoon bieneusi) in intestinal biopsy specimens from human immunodeficiency virus-infected patients by PCR.

Authors:  C Franzen; A Müller; P Hegener; B Salzberger; P Hartmann; G Fätkenheuer; V Diehl; M Schrappe
Journal:  J Clin Microbiol       Date:  1995-09       Impact factor: 5.948
  10 in total
  46 in total

1.  Diagnosis of Enterocytozoon bieneusi by PCR in stool samples eluted from filter paper disks.

Authors:  S Carnevale; J N Velásquez; J H Labbé; A Chertcoff; M G Cabrera; M I Rodríguez
Journal:  Clin Diagn Lab Immunol       Date:  2000-05

2.  In vitro culture, ultrastructure, antigenic, and molecular characterization of Encephalitozoon cuniculi isolated from urine and sputum samples from a Spanish patient with AIDS.

Authors:  C del Aguila; H Moura; S Fenoy; R Navajas; R Lopez-Velez; L Li; L Xiao; G J Leitch; A da Silva; N J Pieniazek; A A Lal; G S Visvesvara
Journal:  J Clin Microbiol       Date:  2001-03       Impact factor: 5.948

3.  Development and application of genetic probes for detection of Enterocytozoon bieneusi in formalin-fixed stools and in intestinal biopsy specimens from infected patients.

Authors:  A Carville; K Mansfield; G Widmer; A Lackner; D Kotler; P Wiest; T Gumbo; S Sarbah; S Tzipori
Journal:  Clin Diagn Lab Immunol       Date:  1997-07

4.  Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa.

Authors:  P A Orlandi; K A Lampel
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

5.  Specific PCR assay for direct detection of intestinal microsporidia Enterocytozoon bieneusi and Encephalitozoon intestinalis in fecal specimens from human immunodeficiency virus-infected patients.

Authors:  C Ombrouck; L Ciceron; S Biligui; S Brown; P Marechal; T van Gool; A Datry; M Danis; I Desportes-Livage
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

6.  Blinded, externally controlled multicenter evaluation of light microscopy and PCR for detection of microsporidia in stool specimens. The Diagnostic Multicenter Study Group on Microsporidia.

Authors:  H Rinder; K Janitschke; H Aspöck; A J Da Silva; P Deplazes; D P Fedorko; C Franzen; U Futh; F Hünger; A Lehmacher; C G Meyer; J M Molina; J Sandfort; R Weber; T Löscher
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

Review 7.  First cases of microsporidiosis in transplant recipients in Spain and review of the literature.

Authors:  A L Galván; A M Martín Sánchez; M A Pérez Valentín; N Henriques-Gil; F Izquierdo; S Fenoy; C del Aguila
Journal:  J Clin Microbiol       Date:  2011-02-16       Impact factor: 5.948

8.  Simultaneous detection of four human pathogenic microsporidian species from clinical samples by oligonucleotide microarray.

Authors:  Zheng Wang; Palmer A Orlandi; David A Stenger
Journal:  J Clin Microbiol       Date:  2005-08       Impact factor: 5.948

9.  Occurrence and genotypic characteristics of Enterocytozoon bieneusi in pigs with diarrhea.

Authors:  Du-Kyung Jeong; Ga-Yeon Won; Bae-Keun Park; Jin Hur; Ju-Yeon You; Su-Jin Kang; In-Gyeong Oh; Yun-Sik Lee; Barry D Stein; John Hwa Lee
Journal:  Parasitol Res       Date:  2007-09-16       Impact factor: 2.289

10.  First detection of microsporidia in dairy calves in North America.

Authors:  R Fayer; M Santín; J M Trout
Journal:  Parasitol Res       Date:  2003-05-09       Impact factor: 2.289
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