Literature DB >> 11679362

Rapid extraction of DNA From Escherichia coli and Cryptosporidium parvum for use in PCR.

J A Higgins1, M C Jenkins, D R Shelton, R Fayer, J S Karns.   

Abstract

The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum.

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Year:  2001        PMID： 11679362      PMCID： PMC93307          DOI： 10.1128/AEM.67.11.5321-5324.2001

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  Diagnosis of Enterocytozoon bieneusi by PCR in stool samples eluted from filter paper disks.

Authors:  S Carnevale; J N Velásquez; J H Labbé; A Chertcoff; M G Cabrera; M I Rodríguez
Journal:  Clin Diagn Lab Immunol       Date:  2000-05

2.  Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa.

Authors:  P A Orlandi; K A Lampel
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

Review 3.  Inhibition and facilitation of nucleic acid amplification.

Authors:  I G Wilson
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

4.  The complete genome sequence of Escherichia coli K-12.

Authors:  F R Blattner; G Plunkett; C A Bloch; N T Perna; V Burland; M Riley; J Collado-Vides; J D Glasner; C K Rode; G F Mayhew; J Gregor; N W Davis; H A Kirkpatrick; M A Goeden; D J Rose; B Mau; Y Shao
Journal:  Science       Date:  1997-09-05       Impact factor: 47.728

5.  Development of a novel, rapid integrated Cryptosporidium parvum detection assay.

Authors:  D Kozwich; K A Johansen; K Landau; C A Roehl; S Woronoff; P A Roehl
Journal:  Appl Environ Microbiol       Date:  2000-07       Impact factor: 4.792

6.  Cloning and expression of a DNA sequence encoding a 41-kilodalton Cryptosporidium parvum oocyst wall protein.

Authors:  M C Jenkins; J Trout; C Murphy; J A Harp; J Higgins; W Wergin; R Fayer
Journal:  Clin Diagn Lab Immunol       Date:  1999-11

7.  Species-specific, nested PCR-restriction fragment length polymorphism detection of single Cryptosporidium parvum oocysts.

Authors:  G D Sturbaum; C Reed; P J Hoover; B H Jost; M M Marshall; C R Sterling
Journal:  Appl Environ Microbiol       Date:  2001-06       Impact factor: 4.792

8.  Detection of cryptosporidia and Cryptosporidium parvum oocysts in environmental water samples by immunomagnetic separation-polymerase chain reaction.

Authors:  S Hallier-Soulier; E Guillot
Journal:  J Appl Microbiol       Date:  2000-07       Impact factor: 3.772

9.  Prevalence of Cryptosporidium, Giardia and Eimeria infections in post-weaned and adult cattle on three Maryland farms.

Authors:  R Fayer; J M Trout; T K Graczyk; E J Lewis
Journal:  Vet Parasitol       Date:  2000-11-10       Impact factor: 2.738

10.  Cryptosporidium parvum in oysters from commercial harvesting sites in the Chesapeake Bay.

Authors:  R Fayer; E J Lewis; J M Trout; T K Graczyk; M C Jenkins; J Higgins; L Xiao; A A Lal
Journal:  Emerg Infect Dis       Date:  1999 Sep-Oct       Impact factor: 6.883
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  4 in total

1.  Recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip for equipment-free detection of Cryptosporidium spp. oocysts in dairy cattle feces.

Authors:  Yao-Dong Wu; Dong-Hui Zhou; Long-Xian Zhang; Wen-Bin Zheng; Jian-Gang Ma; Meng Wang; Xing-Quan Zhu; Min-Jun Xu
Journal:  Parasitol Res       Date:  2016-05-13       Impact factor: 2.289

2.  tir- and stx-positive Escherichia coli in stream waters in a metropolitan area.

Authors:  James A Higgins; Kenneth T Belt; Jeffrey S Karns; Jonathan Russell-Anelli; Daniel R Shelton
Journal:  Appl Environ Microbiol       Date:  2005-05       Impact factor: 4.792

3.  Comparative analysis of the Schleicher and Schuell IsoCode Stix DNA isolation device and the Qiagen QIAamp DNA Mini Kit.

Authors:  Susan R Coyne; Philip D Craw; David A Norwood; Melanie P Ulrich
Journal:  J Clin Microbiol       Date:  2004-10       Impact factor: 5.948

4.  Human DNA extraction from whole saliva that was fresh or stored for 3, 6 or 12 months using five different protocols.

Authors:  Thais Francini Garbieri; Daniel Thomas Brozoski; Thiago José Dionísio; Carlos Ferreira Santos; Lucimara Teixeira das Neves
Journal:  J Appl Oral Sci       Date:  2017 Mar-Apr       Impact factor: 2.698
  4 in total

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