OBJECTIVE: This report describes a new and improved "quick-hot Gram-chromotrope" staining technique that detects microsporidian spores in clinical specimens, such as stool, urine, saliva, nasopharyngeal fluid, and bronchoalveolar lavage samples, as well as in formalin-fixed and paraffin-embedded tissue sections. DESIGN: In this procedure, the samples are stained in heated (50 degrees C to 55 degrees C) solutions of crystal violet and iodine used in Gram's stain, followed by a modified chromotrope solution (heated to 50 degrees C to 55 degrees C). The modified stain is composed of chromotrope 2R (1%), fast green (0.15%), and phosphotungstic acid (0.25%). RESULTS: With this stain and the new protocol, microsporidian spores are stained dark violet against a pale green background, and the total staining time is shortened to 5 minutes. CONCLUSIONS: This new technique is fast, reliable, and simple. It can be easily adapted for use in clinical laboratories.
OBJECTIVE: This report describes a new and improved "quick-hot Gram-chromotrope" staining technique that detects microsporidian spores in clinical specimens, such as stool, urine, saliva, nasopharyngeal fluid, and bronchoalveolar lavage samples, as well as in formalin-fixed and paraffin-embedded tissue sections. DESIGN: In this procedure, the samples are stained in heated (50 degrees C to 55 degrees C) solutions of crystal violet and iodine used in Gram's stain, followed by a modified chromotrope solution (heated to 50 degrees C to 55 degrees C). The modified stain is composed of chromotrope 2R (1%), fast green (0.15%), and phosphotungstic acid (0.25%). RESULTS: With this stain and the new protocol, microsporidian spores are stained dark violet against a pale green background, and the total staining time is shortened to 5 minutes. CONCLUSIONS: This new technique is fast, reliable, and simple. It can be easily adapted for use in clinical laboratories.
Authors: C del Aguila; H Moura; S Fenoy; R Navajas; R Lopez-Velez; L Li; L Xiao; G J Leitch; A da Silva; N J Pieniazek; A A Lal; G S Visvesvara Journal: J Clin Microbiol Date: 2001-03 Impact factor: 5.948
Authors: Lynne S Garcia; Michael Arrowood; Evelyne Kokoskin; Graeme P Paltridge; Dylan R Pillai; Gary W Procop; Norbert Ryan; Robyn Y Shimizu; Govinda Visvesvara Journal: Clin Microbiol Rev Date: 2017-11-15 Impact factor: 26.132
Authors: G P Croppo; G P Croppo; H Moura; A J Da Silva; G J Leitch; D M Moss; S Wallace; S B Slemenda; N J Pieniazek; G S Visvesvara Journal: J Clin Microbiol Date: 1998-05 Impact factor: 5.948