Literature DB >> 7665639

Identification of microsporidia in stool specimens by using PCR and restriction endonucleases.

D P Fedorko1, N A Nelson, C P Cartwright.   

Abstract

We report the development of a PCR-based assay for the detection of microsporidia in clinical specimens. A single primer pair complementary to conserved sequences of the small-subunit rRNA enabled amplification of DNA from the four major microsporidian pathogens of humans: Encephalitozoon cuniculi, Encephalitozoon hellem, Enterocytozoon bieneusi, and Septata intestinalis. The extraction method allowed PCR amplification of E. bieneusi and S. intestinalis DNA from sodium hypochlorite-treated stool specimens. Differentiation of the microsporidian gastrointestinal pathogens E. bieneusi and S. intestinalis could be accomplished by restriction endonuclease digestion of PCR products using PstI and HaeIII.

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Year:  1995        PMID： 7665639      PMCID： PMC228260          DOI： 10.1128/jcm.33.7.1739-1741.1995

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Diagnosis of intestinal microsporidiosis by examination of stool and duodenal aspirate with Weber's modified trichrome and Uvitex 2B strains.

Authors:  P C DeGirolami; C R Ezratty; G Desai; A McCullough; D Asmuth; C Wanke; M Federman
Journal:  J Clin Microbiol       Date:  1995-04       Impact factor: 5.948

2.  Electron microscopic changes in Enterocytozoon bieneusi following treatment with albendazole.

Authors:  C Blanshard; D S Ellis; S P Dowell; G Tovey; B G Gazzard
Journal:  J Clin Pathol       Date:  1993-10       Impact factor: 3.411

3.  Diagnosis of intestinal and disseminated microsporidial infections in patients with HIV by a new rapid fluorescence technique.

Authors:  T van Gool; F Snijders; P Reiss; J K Eeftinck Schattenkerk; M A van den Bergh Weerman; J F Bartelsman; J J Bruins; E U Canning; J Dankert
Journal:  J Clin Pathol       Date:  1993-08       Impact factor: 3.411

4.  A new trichrome-blue stain for detection of microsporidial species in urine, stool, and nasopharyngeal specimens.

Authors:  N J Ryan; G Sutherland; K Coughlan; M Globan; J Doultree; J Marshall; R W Baird; J Pedersen; B Dwyer
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

5.  Treatment with albendazole for intestinal disease due to Enterocytozoon bieneusi in patients with AIDS.

Authors:  D T Dieterich; E A Lew; D P Kotler; M A Poles; J M Orenstein
Journal:  J Infect Dis       Date:  1994-01       Impact factor: 5.226

6.  Septata intestinalis frequently isolated from stool of AIDS patients with a new cultivation method.

Authors:  T van Gool; E U Canning; H Gilis; M A van den Bergh Weerman; J K Eeftinck Schattenkerk; J Dankert
Journal:  Parasitology       Date:  1994-09       Impact factor: 3.234

7.  Improved light-microscopical detection of microsporidia spores in stool and duodenal aspirates. The Enteric Opportunistic Infections Working Group.

Authors:  R Weber; R T Bryan; R L Owen; C M Wilcox; L Gorelkin; G S Visvesvara
Journal:  N Engl J Med       Date:  1992-01-16       Impact factor: 91.245

8.  Detection of microsporidian spores in clinical samples by indirect fluorescent-antibody assay using whole-cell antisera to Encephalitozoon cuniculi and Encephalitozoon hellem.

Authors:  C H Zierdt; V J Gill; W S Zierdt
Journal:  J Clin Microbiol       Date:  1993-11       Impact factor: 5.948

Review 9.  Clinical features of microsporidiosis in patients with AIDS.

Authors:  D M Asmuth; P C DeGirolami; M Federman; C R Ezratty; D K Pleskow; G Desai; C A Wanke
Journal:  Clin Infect Dis       Date:  1994-05       Impact factor: 9.079

10.  Small subunit rRNA sequence of Enterocytozoon bieneusi and its potential diagnostic role with use of the polymerase chain reaction.

Authors:  X Zhu; M Wittner; H B Tanowitz; D Kotler; A Cali; L M Weiss
Journal:  J Infect Dis       Date:  1993-12       Impact factor: 5.226
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  35 in total

1.  Diagnosis of Enterocytozoon bieneusi by PCR in stool samples eluted from filter paper disks.

Authors:  S Carnevale; J N Velásquez; J H Labbé; A Chertcoff; M G Cabrera; M I Rodríguez
Journal:  Clin Diagn Lab Immunol       Date:  2000-05

2.  Species-specific identification of microsporidia in stool and intestinal biopsy specimens by the polymerase chain reaction.

Authors:  N P Kock; H Petersen; T Fenner; I Sobottka; C Schmetz; P Deplazes; N J Pieniazek; H Albrecht; J Schottelius
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1997-05       Impact factor: 3.267

3.  Development and application of genetic probes for detection of Enterocytozoon bieneusi in formalin-fixed stools and in intestinal biopsy specimens from infected patients.

Authors:  A Carville; K Mansfield; G Widmer; A Lackner; D Kotler; P Wiest; T Gumbo; S Sarbah; S Tzipori
Journal:  Clin Diagn Lab Immunol       Date:  1997-07

4.  Specific PCR assay for direct detection of intestinal microsporidia Enterocytozoon bieneusi and Encephalitozoon intestinalis in fecal specimens from human immunodeficiency virus-infected patients.

Authors:  C Ombrouck; L Ciceron; S Biligui; S Brown; P Marechal; T van Gool; A Datry; M Danis; I Desportes-Livage
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

5.  Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA.

Authors:  A J da Silva; D A Schwartz; G S Visvesvara; H de Moura; S B Slemenda; N J Pieniazek
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948

6.  Blinded, externally controlled multicenter evaluation of light microscopy and PCR for detection of microsporidia in stool specimens. The Diagnostic Multicenter Study Group on Microsporidia.

Authors:  H Rinder; K Janitschke; H Aspöck; A J Da Silva; P Deplazes; D P Fedorko; C Franzen; U Futh; F Hünger; A Lehmacher; C G Meyer; J M Molina; J Sandfort; R Weber; T Löscher
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

Review 7.  DNA fingerprinting techniques for microorganisms. A proposal for classification and nomenclature.

Authors:  M Vaneechoutte
Journal:  Mol Biotechnol       Date:  1996-10       Impact factor: 2.695

8.  Real-time PCR method for detection of Encephalitozoon intestinalis from stool specimens.

Authors:  D M Wolk; S K Schneider; N L Wengenack; L M Sloan; J E Rosenblatt
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

9.  Confirmation of the human-pathogenic microsporidia Enterocytozoon bieneusi, Encephalitozoon intestinalis, and Vittaforma corneae in water.

Authors:  S E Dowd; C P Gerba; I L Pepper
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

10.  First detection of microsporidia in dairy calves in North America.

Authors:  R Fayer; M Santín; J M Trout
Journal:  Parasitol Res       Date:  2003-05-09       Impact factor: 2.289
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