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Literature DB >> 10508019

Molecular cloning of the gyrA and gyrB genes of Bacteroides fragilis encoding DNA gyrase.

Abstract

The genes encoding the DNA gyrase A and B subunits of Bacteroides fragilis were cloned and sequenced. The gyrA and gyrB genes code for proteins of 845 and 653 amino acids, respectively. These proteins were expressed in Escherichia coli, and the combination of GyrA and GyrB exhibited ATP-dependent supercoiling activity. To analyze the role of DNA gyrase in quinolone resistance of B. fragilis, we isolated mutant strains by stepwise selection for resistance to increasing concentrations of levofloxacin. We analyzed the resistant mutants and showed that Ser-82 of GyrA, equivalent to resistance hot spot Ser-83 of GyrA in E. coli, was in each case replaced with Phe. These results suggest that DNA gyrase is an important target for quinolones in B. fragilis.

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Year: 1999 PMID： 10508019 PMCID： PMC89495 DOI： 10.1128/AAC.43.10.2423

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

32 in total

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Journal: Science Date: 1979-11-30 Impact factor: 47.728

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Journal: Antimicrob Agents Chemother Date: 1988-07 Impact factor: 5.191

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Journal: Antimicrob Agents Chemother Date: 1990-06 Impact factor: 5.191

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Journal: Antimicrob Agents Chemother Date: 1989-06 Impact factor: 5.191

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Authors: M Tanaka; Y Onodera; Y Uchida; K Sato
Journal: Antimicrob Agents Chemother Date: 1998-11 Impact factor: 5.191

6 in total

1. Role of topoisomerase mutations and efflux in fluoroquinolone resistance of Bacteroides fragilis clinical isolates and laboratory mutants.

Authors: Vito Ricci; Marnie L Peterson; John C Rotschafer; Hannah Wexler; Laura J V Piddock
Journal: Antimicrob Agents Chemother Date: 2004-04 Impact factor: 5.191

2. Genetic determinant of intrinsic quinolone resistance in Fusobacterium canifelinum.

Authors: Georg Conrads; Diane M Citron; Ellie J C Goldstein
Journal: Antimicrob Agents Chemother Date: 2005-01 Impact factor: 5.191

3. Roles of gyrA mutations in resistance of clinical isolates and in vitro mutants of Bacteroides fragilis to the new fluoroquinolone trovafloxacin.

Authors: R Bachoual; L Dubreuil; C J Soussy; J Tankovic
Journal: Antimicrob Agents Chemother Date: 2000-07 Impact factor: 5.191