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Literature DB >> 10427744

Measurement of 3hJNC' connectivities across hydrogen bonds in a 30 kDa protein.

Y X Wang¹, J Jacob, F Cordier, P Wingfield, S J Stahl, S Lee-Huang, D Torchia, S Grzesiek, A Bax.

Abstract

A method is described which permits detection of 3hJNC' scalar couplings across hydrogen bonds in larger, perdeuterated proteins. The experiment is demonstrated for the uniformly 2H/13C/15N-enriched 30 kDa ribosome inactivating protein MAP30. The 3hJNC' interactions are smaller than 1 Hz, but their detection in an HNCO experiment is made possible through the use of constructive interference between the 15N chemical shift anisotropy and 1H-15N dipole-dipole relaxation mechanisms in a manner similar to that of recently proposed TROSY schemes. Sensitivity of the HNCO experiment depends strongly on the 15N transverse relaxation rate of the downfield 15N multiplet component and on the amide proton T1. In perdeuterated MAP30 at 40 degrees C, the average TROSY T2 was 169 ms at 750 MHz 1H frequency, and a wide range of longitudinal relaxation rates was observed for the amide protons.

Entities: Chemical

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Year: 1999 PMID： 10427744 DOI： 10.1023/a:1008346517302

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

6 in total

1. Gradient and sensitivity enhancement of 2D TROSY with water flip-back, 3D NOESY-TROSY and TOCSY-TROSY experiments.

Authors: G Zhu; X M Kong; K H Sze
Journal: J Biomol NMR Date: 1999-01 Impact factor: 2.835

2. NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors: F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal: J Biomol NMR Date: 1995-11 Impact factor: 2.835

3. Single Transition-to-single Transition Polarization Transfer (ST2-PT) in [15N,1H]-TROSY.

Authors: K V Pervushin; G Wider; K Wüthrich
Journal: J Biomol NMR Date: 1998-08 Impact factor: 2.835

4. Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution.

Authors: K Pervushin; R Riek; G Wider; K Wüthrich
Journal: Proc Natl Acad Sci U S A Date: 1997-11-11 Impact factor: 11.205

5. Improved 1HN-detected triple resonance TROSY-based experiments.

Authors: D Yang; L E Kay
Journal: J Biomol NMR Date: 1999-01 Impact factor: 2.835

6. NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by transverse relaxation-optimized spectroscopy.

Authors: K Pervushin; A Ono; C Fernández; T Szyperski; M Kainosho; K Wüthrich
Journal: Proc Natl Acad Sci U S A Date: 1998-11-24 Impact factor: 11.205

6 in total

24 in total

1. Modeling 2hJiso(N, N) in nucleic acid base pairs: ab initio characterization of the 2hJ(N, N) tensor in the methyleneimine dimer as a function of hydrogen bond geometry.

Authors: D L Bryce; R E Wasylishen
Journal: J Biomol NMR Date: 2001-04 Impact factor: 2.835

2. Simultaneous measurement of protein one-bond and two-bond nitrogen-carbon coupling constants using an internally referenced quantitative J-correlated [(15)N,(1)H]-TROSY-HNC experiment.

Authors: Hans L J Wienk; Mitcheell M Martínez; Gary N Yalloway; Jürgen M Schmidt; Carlos Pérez; Heinz Rüterjans; Frank Löhr
Journal: J Biomol NMR Date: 2003-02 Impact factor: 2.835

Review 3. Weak alignment offers new NMR opportunities to study protein structure and dynamics.

Authors: Ad Bax
Journal: Protein Sci Date: 2003-01 Impact factor: 6.725

4. HIFI-C: a robust and fast method for determining NMR couplings from adaptive 3D to 2D projections.

Authors: Gabriel Cornilescu; Arash Bahrami; Marco Tonelli; John L Markley; Hamid R Eghbalnia
Journal: J Biomol NMR Date: 2007-07-04 Impact factor: 2.835

5. Characterization of the hydrogen bond network in guanosine quartets by internucleotide 3hJ(NC)' and 2hJ(NN) scalar couplings.

Authors: A J Dingley; J E Masse; J Feigon; S Grzesiek
Journal: J Biomol NMR Date: 2000-04 Impact factor: 2.835

6. Anti-HIV and anti-tumor protein MAP30, a 30 kDa single-strand type-I RIP, shares similar secondary structure and beta-sheet topology with the A chain of ricin, a type-II RIP.

Authors: Y X Wang; J Jacob; P T Wingfield; I Palmer; S J Stahl; J D Kaufman; P L Huang; P L Huang; S Lee-Huang; D A Torchia
Journal: Protein Sci Date: 2000-01 Impact factor: 6.725

7. Detection of very weak side chain-main chain hydrogen bonding interactions in medium-size 13C/15N-labeled proteins by sensitivity-enhanced NMR spectroscopy.

Authors: A Liu; W Hu; A Majumdar; M K Rosen; D J Patel
Journal: J Biomol NMR Date: 2000-05 Impact factor: 2.835

8. Sensitivity enhanced NMR spectroscopy by quenching scalar coupling mediated relaxation: application to the direct observation of hydrogen bonds in 13C/15N-labeled proteins.

Authors: A Liu; W Hu; S Qamar; A Majumdar
Journal: J Biomol NMR Date: 2000-05 Impact factor: 2.835

9. Solution structure of the N-domain of Wilson disease protein: distinct nucleotide-binding environment and effects of disease mutations.

Authors: Oleg Dmitriev; Ruslan Tsivkovskii; Frits Abildgaard; Clinton T Morgan; John L Markley; Svetlana Lutsenko
Journal: Proc Natl Acad Sci U S A Date: 2006-03-27 Impact factor: 11.205

10. Direct NMR observation and DFT calculations of a hydrogen bond at the active site of a 44 kDa enzyme.

Authors: Alexander Eletsky; Tim Heinz; Osvaldo Moreira; Alexander Kienhöfer; Donald Hilvert; Konstantin Pervushi
Journal: J Biomol NMR Date: 2002-09 Impact factor: 2.835