Literature DB >> 10909868

Detection of very weak side chain-main chain hydrogen bonding interactions in medium-size 13C/15N-labeled proteins by sensitivity-enhanced NMR spectroscopy.

A Liu1, W Hu, A Majumdar, M K Rosen, D J Patel.   

Abstract

We describe the direct observation of very weak side chain-main chain hydrogen bonding interactions in medium-size 13C/15N-labeled proteins with sensitivity-enhanced NMR spectroscopy. Specifically, the remote correlation. between the hydrogen acceptor side chain carboxylate carbon 13CO2delta of glutamate 54 and the hydrogen donor backbone amide 15N of methionine 49 in a 12 kDa protein, human FKBP12, is detected via the trans-hydrogen bond 3hJ(NCO2delta) coupling by employing a novel sensitivity-enhanced HNCO-type experiment, CPD-HNCO. The 3hJ(NCO2delta) coupling constant appears to be even smaller than the average value of backbone 3hJ(NC') couplings, consistent with more extensive local dynamics in protein side chains.

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Year:  2000        PMID： 10909868     DOI： 10.1023/a:1008373501591

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  10 in total

1.  Observation of through-hydrogen-bond 2hJHC' in a perdeuterated protein.

Authors:  F Cordier; M Rogowski; S Grzesiek; A Bax
Journal:  J Magn Reson       Date:  1999-10       Impact factor: 2.229

2.  Sensitivity enhanced NMR spectroscopy by quenching scalar coupling mediated relaxation: application to the direct observation of hydrogen bonds in 13C/15N-labeled proteins.

Authors:  A Liu; W Hu; S Qamar; A Majumdar
Journal:  J Biomol NMR       Date:  2000-05       Impact factor: 2.835

3.  Observation and measurement of internucleotide 2JNN coupling constants between 15N nuclei with widely separated chemical shifts.

Authors:  A Majumdar; A Kettani; E Skripkin
Journal:  J Biomol NMR       Date:  1999-05       Impact factor: 2.835

4.  Measurement of 3hJNC' connectivities across hydrogen bonds in a 30 kDa protein.

Authors:  Y X Wang; J Jacob; F Cordier; P Wingfield; S J Stahl; S Lee-Huang; D Torchia; S Grzesiek; A Bax
Journal:  J Biomol NMR       Date:  1999-06       Impact factor: 2.835

5.  Observation of internucleotide NH...N hydrogen bonds in the absence of directly detectable protons.

Authors:  A Majumdar; A Kettani; E Skripkin; D J Patel
Journal:  J Biomol NMR       Date:  1999-11       Impact factor: 2.835

6.  NMR experiments for resonance assignments of 13C, 15N doubly-labeled flexible polypeptides: application to the human prion protein hPrP(23-230).

Authors:  A Liu; R Riek; G Wider; C von Schroetter; R Zahn; K Wüthrich
Journal:  J Biomol NMR       Date:  2000-02       Impact factor: 2.835

7.  Proton and nitrogen sequential assignments and secondary structure determination of the human FK506 and rapamycin binding protein.

Authors:  M K Rosen; S W Michnick; M Karplus; S L Schreiber
Journal:  Biochemistry       Date:  1991-05-14       Impact factor: 3.162

8.  The 2D NMR experiments H(C)CO2 and HCCO2 for assignment and pH titration of carboxylate groups in uniformly 15N/13C-labeled proteins.

Authors:  M Pellecchia; H Iwai; T Szyperski; K Wüthrich
Journal:  J Magn Reson       Date:  1997-01       Impact factor: 2.229

9.  1H, 13C, and 15N assignments and secondary structure of the FK506 binding protein when bound to ascomycin.

Authors:  R X Xu; D Nettesheim; E T Olejniczak; R Meadows; G Gemmecker; S W Fesik
Journal:  Biopolymers       Date:  1993-04       Impact factor: 2.505

10.  NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by transverse relaxation-optimized spectroscopy.

Authors:  K Pervushin; A Ono; C Fernández; T Szyperski; M Kainosho; K Wüthrich
Journal:  Proc Natl Acad Sci U S A       Date:  1998-11-24       Impact factor: 11.205
  10 in total
  6 in total

1.  Mapping membrane protein backbone dynamics: a comparison of site-directed spin labeling with NMR 15N-relaxation measurements.

Authors:  Ryan H Lo; Brett M Kroncke; Tsega L Solomon; Linda Columbus
Journal:  Biophys J       Date:  2014-10-07       Impact factor: 4.033

2.  NMR detection of side chain-side chain hydrogen bonding interactions in 13C/15N-labeled proteins.

Authors:  A Liu; W Hu; A Majumdar; M K Rosen; D J Patel
Journal:  J Biomol NMR       Date:  2000-08       Impact factor: 2.835

Review 3.  Conformational Dynamics in FKBP Domains: Relevance to Molecular Signaling and Drug Design.

Authors:  David M LeMaster; Griselda Hernandez
Journal:  Curr Mol Pharmacol       Date:  2015       Impact factor: 3.339

4.  Rapid protein fold determination using secondary chemical shifts and cross-hydrogen bond 15N-13C' scalar couplings (3hbJNC').

Authors:  A M Bonvin; K Houben; M Guenneugues; R Kaptein; R Boelens
Journal:  J Biomol NMR       Date:  2001-11       Impact factor: 2.835

5.  Direct NMR observation and DFT calculations of a hydrogen bond at the active site of a 44 kDa enzyme.

Authors:  Alexander Eletsky; Tim Heinz; Osvaldo Moreira; Alexander Kienhöfer; Donald Hilvert; Konstantin Pervushi
Journal:  J Biomol NMR       Date:  2002-09       Impact factor: 2.835

6.  Analysing the visible conformational substates of the FK506-binding protein FKBP12.

Authors:  Sourajit M Mustafi; Hui Chen; Hongmin Li; David M Lemaster; Griselda Hernández
Journal:  Biochem J       Date:  2013-08-01       Impact factor: 3.857
  6 in total

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