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Literature DB >> 9691285

Heteronuclear correlation experiments for the determination of one-bond coupling constants.

P Andersson¹, K Nordstrand, M Sunnerhagen, E Liepinsh, I Turovskis, G Otting.

Abstract

Spin-state selective experiments, HSQC-alpha/beta and CT-HMQC-alpha/beta, are proposed for the simple and rapid measurement of scalar one-bond coupling constants in two-dimensional, 1H-detected 15N-1H or 13C-1H correlation experiments based on HSQC and HMQC schemes. Pairs of subspectra are obtained, containing either the high-field or the low-field component of the doublet representing the one-bond coupling constant. The subspectral editing procedure retains the full sensitivity of HSQC and HMQC spectra recorded without heteronuclear decoupling during data acquisition, with a spectral resolution similar to that of decoupled spectra.

Entities: Chemical

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Year: 1998 PMID： 9691285 DOI： 10.1023/a:1008206212145

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

14 in total

1. Integration of spin-state-selective excitation into 2D NMR correlation experiments with the heteronuclear ZQ/2Q pi rotations for 1JXH- resolved E.COSY-type measurements of heteronuclear coupling constants in proteins.

Authors: A Meissner; J O Duus; O W Sørensen
Journal: J Biomol NMR Date: 1997-07 Impact factor: 2.835

2. Measurement of dipolar contributions to 1JCH splittings from magnetic-field dependence of J modulation in two-dimensional NMR spectra.

Authors: N Tjandra; A Bax
Journal: J Magn Reson Date: 1997-02 Impact factor: 2.229

3. NMR evidence for slow collective motions in cyanometmyoglobin.

Authors: J R Tolman; J M Flanagan; M A Kennedy; J H Prestegard
Journal: Nat Struct Biol Date: 1997-04

4. The use of 1JC alpha H alpha coupling constants as a probe for protein backbone conformation.

Authors: G W Vuister; F Delaglio; A Bax
Journal: J Biomol NMR Date: 1993-01 Impact factor: 2.835

5. Calculations of one-, two- and three-bond nuclear spin-spin couplings in a model peptide and correlations with experimental data.

Authors: A S Edison; J L Markley; F Weinhold
Journal: J Biomol NMR Date: 1994-07 Impact factor: 2.835

6. Estimates of phi and psi torsion angles in proteins from one-, two- and three-bond nuclear spin-spin couplings: application to staphylococcal nuclease.

Authors: A S Edison; F Weinhold; W M Westler; J L Markley
Journal: J Biomol NMR Date: 1994-07 Impact factor: 2.835

7. Solution structure of the DNA-binding domain and model for the complex of multifunctional hexameric arginine repressor with DNA.

Authors: M Sunnerhagen; M Nilges; G Otting; J Carey
Journal: Nat Struct Biol Date: 1997-10

Heteronuclear correlation experiments for the determination of one-bond coupling constants.

1. Integration of spin-state-selective excitation into 2D NMR correlation experiments with the heteronuclear ZQ/2Q pi rotations for 1JXH- resolved E.COSY-type measurements of heteronuclear coupling constants in proteins.

2. Measurement of dipolar contributions to 1JCH splittings from magnetic-field dependence of J modulation in two-dimensional NMR spectra.

3. NMR evidence for slow collective motions in cyanometmyoglobin.

4. The use of 1JC alpha H alpha coupling constants as a probe for protein backbone conformation.

5. Calculations of one-, two- and three-bond nuclear spin-spin couplings in a model peptide and correlations with experimental data.

6. Estimates of phi and psi torsion angles in proteins from one-, two- and three-bond nuclear spin-spin couplings: application to staphylococcal nuclease.

7. Solution structure of the DNA-binding domain and model for the complex of multifunctional hexameric arginine repressor with DNA.

8. Spin-locked multiple quantum coherence for signal enhancement in heteronuclear multidimensional NMR experiments.

9. Cross-correlation effects on NMR lineshapes and peptide conformation.

10. A general enhancement scheme in heteronuclear multidimensional NMR employing pulsed field gradients.

1. Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings.

2. A doublet-separated sensitivity-enhanced HSQC for the determination of scalar and dipolar one-bond J-couplings.

3. Detection of C',Calpha correlations in proteins using a new time- and sensitivity-optimal experiment.

4. Spin-state selection filters for the measurement of heteronuclear one-bond coupling constants.

5. Structure of stem-loop IV of Tetrahymena telomerase RNA.

6. A small-molecule probe induces a conformation in HIV TAR RNA capable of binding drug-like fragments.

7. Structural basis for stabilization of the tau pre-mRNA splicing regulatory element by novantrone (mitoxantrone).