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Literature DB >> 9916036

Harmonic behavior of trehalose-coated carbon-monoxy-myoglobin at high temperature.

L Cordone¹, M Ferrand, E Vitrano, G Zaccai.

Abstract

Embedding biostructures in saccharide glasses protects them against extreme dehydration and/or exposure to very high temperature. Among the saccharides, trehalose appears to be the most effective bioprotectant. In this paper we report on the low-frequency dynamics of carbon monoxy myoglobin in an extremely dry trehalose glass measured by neutron spectroscopy. Under these conditions, the mean square displacements and the density of state function are those of a harmonic solid, up to room temperature, in contrast to D2O-hydrated myoglobin, in which a dynamical transition to a nonharmonic regime has been observed at approximately 180 K (Doster et al., 1989. Nature. 337:754-756). The protective effect of trehalose is correlated, therefore, with a trapping of the protein in a harmonic potential, even at relatively high temperature.

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Year: 1999 PMID： 9916036 PMCID： PMC1300054 DOI： 10.1016/S0006-3495(99)77269-3

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

16 in total

1. A reduction of protein specific motions in co-ligated myoglobin embedded in a trehalose glass.

Authors: L Cordone; P Galajda; E Vitrano; A Gassmann; A Ostermann; F Parak
Journal: Eur Biophys J Date: 1998 Impact factor: 1.733

2. Protein dynamics. Vibrational coupling, spectral broadening mechanisms, and anharmonicity effects in carbonmonoxy heme proteins studied by the temperature dependence of the Soret band lineshape.

Authors: A Di Pace; A Cupane; M Leone; E Vitrano; L Cordone
Journal: Biophys J Date: 1992-08 Impact factor: 4.033

3. Temperature dependence of the low frequency dynamics of myoglobin. Measurement of the vibrational frequency distribution by inelastic neutron scattering.

Authors: S Cusack; W Doster
Journal: Biophys J Date: 1990-07 Impact factor: 4.033

Review 4. Conformational substates in proteins.

Authors: H Frauenfelder; F Parak; R D Young
Journal: Annu Rev Biophys Biophys Chem Date: 1988

5. Dynamical transition of myoglobin revealed by inelastic neutron scattering.

Authors: W Doster; S Cusack; W Petry
Journal: Nature Date: 1989-02-23 Impact factor: 49.962

6. Protein dynamics. Mössbauer spectroscopy on deoxymyoglobin crystals.

Authors: F Parak; E W Knapp; D Kucheida
Journal: J Mol Biol Date: 1982-10-15 Impact factor: 5.469

7. Protective effect of disaccharides on restriction endonucleases during drying under vacuum.

Authors: M Uritani; M Takai; K Yoshinaga
Journal: J Biochem Date: 1995-04 Impact factor: 3.387

Review 8. Trehalose metabolism--new horizons in technological applications.

Authors: A D Panek
Journal: Braz J Med Biol Res Date: 1995-02 Impact factor: 2.590

9. Preservation of membranes in anhydrobiotic organisms: the role of trehalose.

Authors: J H Crowe; L M Crowe; D Chapman
Journal: Science Date: 1984-02-17 Impact factor: 47.728

10. Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying.

Authors: S B Leslie; E Israeli; B Lighthart; J H Crowe; L M Crowe
Journal: Appl Environ Microbiol Date: 1995-10 Impact factor: 4.792

47 in total

1. Protein flexibility from the dynamical transition: a force constant analysis.

Authors: D J Bicout; G Zaccai
Journal: Biophys J Date: 2001-03 Impact factor: 4.033

2. Electron transfer kinetics in photosynthetic reaction centers embedded in trehalose glasses: trapping of conformational substates at room temperature.

Authors: Gerardo Palazzo; Antonia Mallardi; Alejandro Hochkoeppler; Lorenzo Cordone; Giovanni Venturoli
Journal: Biophys J Date: 2002-02 Impact factor: 4.033

10. Residual water modulates QA- -to-QB electron transfer in bacterial reaction centers embedded in trehalose amorphous matrices.

Authors: Francesco Francia; Gerardo Palazzo; Antonia Mallardi; Lorenzo Cordone; Giovanni Venturoli
Journal: Biophys J Date: 2003-10 Impact factor: 4.033

Harmonic behavior of trehalose-coated carbon-monoxy-myoglobin at high temperature.

1. A reduction of protein specific motions in co-ligated myoglobin embedded in a trehalose glass.

2. Protein dynamics. Vibrational coupling, spectral broadening mechanisms, and anharmonicity effects in carbonmonoxy heme proteins studied by the temperature dependence of the Soret band lineshape.

3. Temperature dependence of the low frequency dynamics of myoglobin. Measurement of the vibrational frequency distribution by inelastic neutron scattering.

Review 4. Conformational substates in proteins.

5. Dynamical transition of myoglobin revealed by inelastic neutron scattering.

6. Protein dynamics. Mössbauer spectroscopy on deoxymyoglobin crystals.

7. Protective effect of disaccharides on restriction endonucleases during drying under vacuum.

Review 8. Trehalose metabolism--new horizons in technological applications.

9. Preservation of membranes in anhydrobiotic organisms: the role of trehalose.

10. Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying.

1. Protein flexibility from the dynamical transition: a force constant analysis.

2. Electron transfer kinetics in photosynthetic reaction centers embedded in trehalose glasses: trapping of conformational substates at room temperature.

3. Radially softening diffusive motions in a globular protein.

4. Temperature dependence of protein dynamics: computer simulation analysis of neutron scattering properties.

5. Solvent dependence of dynamic transitions in protein solutions.

6. Dynamic transition associated with the thermal denaturation of a small Beta protein.

7. Picosecond internal dynamics of lysozyme as affected by thermal unfolding in nonaqueous environment.

8. Effect of the environment on the protein dynamical transition: a neutron scattering study.

9. Non-Gaussian statistics of the vibrational fluctuations of myoglobin.

10. Residual water modulates QA- -to-QB electron transfer in bacterial reaction centers embedded in trehalose amorphous matrices.