Literature DB >> 9819816

Direct sequencing of long polymerase chain reaction fragments.

F Iannelli1, L Giunti, G Pozzi.   

Abstract

Direct sequencing of polymerase chain reaction (PCR)-generated templates is a commonly used technique in molecular biology laboratories. We describe an improved method for direct sequencing of PCR fragments longer than 20 kb obtained with a commercial mixture of Taq and Pwo DNA polymerases. The sequencing protocol was optimized for an automated infrared DNA sequencer, consistently yielding long reads (500-600 bases).

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Year:  1998        PMID： 9819816     DOI： 10.1007/BF02760864

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  8 in total

1.  Continuous, on-line DNA sequencing using a versatile infrared laser scanner/electrophoresis apparatus.

Authors:  L R Middendorf; J C Bruce; R C Bruce; R D Eckles; D L Grone; S C Roemer; G D Sloniker; D L Steffens; S L Sutter; J A Brumbaugh
Journal:  Electrophoresis       Date:  1992-08       Impact factor: 3.535

2.  PCR amplification of streptococcal DNA using crude cell lysates.

Authors:  W L Hynes; J J Ferretti; M S Gilmore; R A Segarra
Journal:  FEMS Microbiol Lett       Date:  1992-07-01       Impact factor: 2.742

3.  Rapid polymerase chain reaction amplification using intact bacterial cells.

Authors:  A K Joshi; V Baichwal; G F Ames
Journal:  Biotechniques       Date:  1991-01       Impact factor: 1.993

4.  Shedding light on PCR contamination.

Authors:  G Sarkar; S S Sommer
Journal:  Nature       Date:  1990-01-04       Impact factor: 49.962

5.  Direct primer walking on P1 plasmid DNA.

Authors:  V Benes; C Kilger; H Voss; S Pääbo; W Ansorge
Journal:  Biotechniques       Date:  1997-07       Impact factor: 1.993

Review 6.  Instability and decay of the primary structure of DNA.

Authors:  T Lindahl
Journal:  Nature       Date:  1993-04-22       Impact factor: 49.962

7.  Cloning of Streptococcus pneumoniae DNA fragments in Escherichia coli requires vectors protected by strong transcriptional terminators.

Authors:  J D Chen; D A Morrison
Journal:  Gene       Date:  1987       Impact factor: 3.688

8.  The difficulty of cloning Streptococcus pneumoniae mal and ami loci in Escherichia coli: toxicity of malX and amiA gene products.

Authors:  B Martin; G Alloing; C Boucraut; J P Claverys
Journal:  Gene       Date:  1989-08-15       Impact factor: 3.688
  8 in total
  6 in total

1.  Drug resistance evolution of a Mycobacterium tuberculosis strain from a noncompliant patient.

Authors:  Francesca Meacci; Germano Orrù; Elisabetta Iona; Federico Giannoni; Claudio Piersimoni; Gianni Pozzi; Lanfranco Fattorini; Marco R Oggioni
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

2.  Characterization of a genetic element carrying the macrolide efflux gene mef(A) in Streptococcus pneumoniae.

Authors:  M Santagati; F Iannelli; M R Oggioni; S Stefani; G Pozzi
Journal:  Antimicrob Agents Chemother       Date:  2000-09       Impact factor: 5.191

3.  Method for introducing specific and unmarked mutations into the chromosome of Streptococcus pneumoniae.

Authors:  Francesco Iannelli; Gianni Pozzi
Journal:  Mol Biotechnol       Date:  2004-01       Impact factor: 2.695

4.  The type 2 capsule locus of Streptococcus pneumoniae.

Authors:  F Iannelli; B J Pearce; G Pozzi
Journal:  J Bacteriol       Date:  1999-04       Impact factor: 3.490

5.  Usefulness of real time PCR for the differentiation and quantification of 652 and JP2 Actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva.

Authors:  Germano Orrù; Mario Francesco Marini; Maria Laura Ciusa; Daniela Isola; Marina Cotti; Marco Baldoni; Vincenzo Piras; Elisabetta Pisano; Caterina Montaldo
Journal:  BMC Infect Dis       Date:  2006-06-13       Impact factor: 3.090

6.  Excision and Circularization of Integrative Conjugative Element Tn5253 of Streptococcus pneumoniae.

Authors:  Francesco Santoro; Alessandra Romeo; Gianni Pozzi; Francesco Iannelli
Journal:  Front Microbiol       Date:  2018-07-31       Impact factor: 5.640
  6 in total

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