Literature DB >> 2003920

Rapid polymerase chain reaction amplification using intact bacterial cells.

A K Joshi1, V Baichwal, G F Ames.   

Abstract

We have demonstrated that efficient polymerase chain reaction amplifications from chromosomal DNA can be carried out using whole bacterial cells as the starting material. Cells from the liquid or solid cultures can be used directly, without any pre-treatment, thus eliminating the need for DNA isolation.

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Year:  1991        PMID： 2003920

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  16 in total

1.  Fingerprinting of cyanobacteria based on PCR with primers derived from short and long tandemly repeated repetitive sequences.

Authors:  U Rasmussen; M M Svenning
Journal:  Appl Environ Microbiol       Date:  1998-01       Impact factor: 4.792

2.  Direct sequencing of long polymerase chain reaction fragments.

Authors:  F Iannelli; L Giunti; G Pozzi
Journal:  Mol Biotechnol       Date:  1998-10       Impact factor: 2.695

3.  Construction of a cDNA encoding the multifunctional animal fatty acid synthase and expression in Spodoptera frugiperda cells using baculoviral vectors.

Authors:  A K Joshi; S Smith
Journal:  Biochem J       Date:  1993-11-15       Impact factor: 3.857

4.  Diversity of the P2 protein among nontypeable Haemophilus influenzae isolates.

Authors:  J Bell; S Grass; D Jeanteur; R S Munson
Journal:  Infect Immun       Date:  1994-06       Impact factor: 3.441

5.  Ribosome recycling factor (ribosome releasing factor) is essential for bacterial growth.

Authors:  L Janosi; I Shimizu; A Kaji
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-10       Impact factor: 11.205

6.  Roles of LysP and CadC in mediating the lysine requirement for acid induction of the Escherichia coli cad operon.

Authors:  M N Neely; C L Dell; E R Olson
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

7.  cobU-dependent assimilation of nonadenosylated cobinamide in cobA mutants of Salmonella typhimurium.

Authors:  G A O'Toole; J C Escalante-Semerena
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490

8.  Restriction fragment length polymorphism analysis of 16S ribosomal DNA of Streptococcus and Enterococcus species of bovine origin.

Authors:  B M Jayarao; J J Doré; S P Oliver
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

9.  Variable region sequences and idiotypic expression of a protective human immunoglobulin M antibody to capsular polysaccharides of Neisseria meningitidis group B and Escherichia coli K1.

Authors:  F H Azmi; A H Lucas; H V Raff; D M Granoff
Journal:  Infect Immun       Date:  1994-05       Impact factor: 3.441

10.  Randomly amplified polymorphic DNA analysis of starved and viable but nonculturable Vibrio vulnificus cells.

Authors:  J M Warner; J D Oliver
Journal:  Appl Environ Microbiol       Date:  1998-08       Impact factor: 4.792
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