Literature DB >> 9812374

A system for heterologous expression of bacteriocins in Lactobacillus sake.

L Axelsson1, T Katla, M Bjørnslett, V G Eijsink, A Holck.   

Abstract

A system for efficient heterologous expression of class II bacteriocins is described that is based on introducing two plasmids in a bacteriocin-negative Lactobacillus sake strain. The first plasmid (pSAK20) contains the genes necessary for transcriptional activation of the Sakacin A promoter as well as export and processing of bacteriocin precursors. The second plasmid (a pLPV111 derivative) contains the structural and immunity genes for the bacteriocin of interest fused to the sakacin A promoter. Using this system, various bacteriocins were produced at levels equal to or higher than those obtained with the corresponding wild-type producer strains.

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Year:  1998        PMID： 9812374     DOI： 10.1111/j.1574-6968.1998.tb13266.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  25 in total

1.  A C-terminal disulfide bridge in pediocin-like bacteriocins renders bacteriocin activity less temperature dependent and is a major determinant of the antimicrobial spectrum.

Authors:  G Fimland; L Johnsen; L Axelsson; M B Brurberg; I F Nes; V G Eijsink; J Nissen-Meyer
Journal:  J Bacteriol       Date:  2000-05       Impact factor: 3.490

2.  Engineering increased stability in the antimicrobial peptide pediocin PA-1.

Authors:  L Johnsen; G Fimland; V Eijsink; J Nissen-Meyer
Journal:  Appl Environ Microbiol       Date:  2000-11       Impact factor: 4.792

3.  Rapid two-step procedure for large-scale purification of pediocin-like bacteriocins and other cationic antimicrobial peptides from complex culture medium.

Authors:  Marianne Uteng; Håvard Hildeng Hauge; Ilia Brondz; Jon Nissen-Meyer; Gunnar Fimland
Journal:  Appl Environ Microbiol       Date:  2002-02       Impact factor: 4.792

4.  An extracellular loop of the mannose phosphotransferase system component IIC is responsible for specific targeting by class IIa bacteriocins.

Authors:  Morten Kjos; Zhian Salehian; Ingolf F Nes; Dzung B Diep
Journal:  J Bacteriol       Date:  2010-09-24       Impact factor: 3.490

5.  Influence of amino acid substitutions in the leader peptide on maturation and secretion of mesentericin Y105 by Leuconostoc mesenteroides.

Authors:  Willy Aucher; Christian Lacombe; Arnaud Héquet; Jacques Frère; Jean-Marc Berjeaud
Journal:  J Bacteriol       Date:  2005-03       Impact factor: 3.490

Review 6.  The continuing story of class IIa bacteriocins.

Authors:  Djamel Drider; Gunnar Fimland; Yann Héchard; Lynn M McMullen; Hervé Prévost
Journal:  Microbiol Mol Biol Rev       Date:  2006-06       Impact factor: 11.056

7.  Construction of plasmid vector for expression of bacteriocin N15-encoding gene and effect of engineered bacteria on Enterococcus faecalis.

Authors:  Monthon Lertcanawanichakul
Journal:  Curr Microbiol       Date:  2007-01-02       Impact factor: 2.188

8.  The lactococcin G immunity protein recognizes specific regions in both peptides constituting the two-peptide bacteriocin lactococcin G.

Authors:  Camilla Oppegård; Linda Emanuelsen; Lisbeth Thorbek; Gunnar Fimland; Jon Nissen-Meyer
Journal:  Appl Environ Microbiol       Date:  2009-12-28       Impact factor: 4.792

9.  Mutational analysis of mesentericin y105, an anti-Listeria bacteriocin, for determination of impact on bactericidal activity, in vitro secondary structure, and membrane interaction.

Authors:  Dany Morisset; Jean-Marc Berjeaud; Didier Marion; Christian Lacombe; Jacques Frère
Journal:  Appl Environ Microbiol       Date:  2004-08       Impact factor: 4.792

10.  Functional characterization of a composite bacteriocin locus from malt isolate Lactobacillus sakei 5.

Authors:  Anne Vaughan; Vincent G H Eijsink; Douwe Van Sinderen
Journal:  Appl Environ Microbiol       Date:  2003-12       Impact factor: 4.792
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