| Literature DB >> 9774615 |
W R Kirkpatrick1, T M Turner, A W Fothergill, D I McCarthy, S W Redding, M G Rinaldi, T F Patterson.
Abstract
We describe a simple procedure for detecting fluconazole-resistant yeasts by a disk diffusion method. Forty clinical Candida sp. isolates were tested on RPMI-glucose agar with either 25- or 50-microgram fluconazole disks. With 25-microgram disks, zones of inhibition of >/=20 mm at 24 h accurately identified 29 of 29 isolates for which MICs were </=8 microgram/ml, and with 50-microgram disks, zones of >/=27 mm identified 28 of 29 such isolates. All 11 isolates for which MICs were >8 microgram/ml were identified by using either disk. Disk diffusion may be a useful screening method for clinical microbiology laboratories.Entities:
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Year: 1998 PMID: 9774615 PMCID: PMC105351
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948