Literature DB >> 9679294

Measurement of three-bond, 13C'-13C beta J couplings in human ubiquitin by a triple resonance, E. COSY-type NMR technique.

J S Hu1, A Bax.   

Abstract

A [CO]HN(CA)CB-E.COSY pulse scheme is described for measurement of three-bond couplings, 3JC'C beta, between carbonyl and aliphatic C beta carbons in ubiquitin, uniformly enriched with 13C and 15N. A Karplus relation, 3JC'C beta = 1.28 cos2(phi - 120 degrees) -1.02 cos(phi - 120 degrees) + 0.30 Hz, is obtained by correlating the 3JC'C beta values measured for human ubiquitin with backbone phi angles from its crystal structure. As predicted, the new Karplus parametrization yields 3JC'C beta values slightly larger than previously obtained by quantitative J correlation [Hu, J.-S. and Bax, A. (1997) J. Am. Chem. Soc., 119, 6360-6368], but considerably smaller than what has been reported on the basis of other E.COSY-type measurements carried out on flavodoxin.

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Year:  1998        PMID： 9679294     DOI： 10.1023/a:1008241904271

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  6 in total

1.  Sequential 1H NMR assignments and secondary structure identification of human ubiquitin.

Authors:  P L Weber; S C Brown; L Mueller
Journal:  Biochemistry       Date:  1987-11-17       Impact factor: 3.162

2.  Two-dimensional 1H NMR study of human ubiquitin: a main chain directed assignment and structure analysis.

Authors:  D L Di Stefano; A J Wand
Journal:  Biochemistry       Date:  1987-11-17       Impact factor: 3.162

3.  Structure of ubiquitin refined at 1.8 A resolution.

Authors:  S Vijay-Kumar; C E Bugg; W J Cook
Journal:  J Mol Biol       Date:  1987-04-05       Impact factor: 5.469

4.  NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors:  F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
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5.  Sequential backbone assignment of isotopically enriched proteins in D2O by deuterium-decoupled HA(CA)N and HA(CACO)N.

Authors:  A C Wang; S Grzesiek; R Tschudin; P J Lodi; A Bax
Journal:  J Biomol NMR       Date:  1995-06       Impact factor: 2.835

6.  Fast internal main-chain dynamics of human ubiquitin.

Authors:  D M Schneider; M J Dellwo; A J Wand
Journal:  Biochemistry       Date:  1992-04-14       Impact factor: 3.162
  6 in total
  7 in total

1.  HYPER: a hierarchical algorithm for automatic determination of protein dihedral-angle constraints and stereospecific C beta H2 resonance assignments from NMR data.

Authors:  R Tejero; D Monleon; B Celda; R Powers; G T Montelione
Journal:  J Biomol NMR       Date:  1999-11       Impact factor: 2.835

2.  Self-consistent 3J coupling analysis for the joint calibration of Karplus coefficients and evaluation of torsion angles.

Authors:  J M Schmidt; M Blümel; F Löhr; H Rüterjans
Journal:  J Biomol NMR       Date:  1999-05       Impact factor: 2.835

3.  Analysis of sub-tauc and supra-tauc motions in protein Gbeta1 using molecular dynamics simulations.

Authors:  Jennifer M Bui; Jörg Gsponer; Michele Vendruscolo; Christopher M Dobson
Journal:  Biophys J       Date:  2009-11-04       Impact factor: 4.033

4.  How uniform is the peptide plane geometry? A high-accuracy NMR study of dipolar Cα-C'/H N-N cross-correlated relaxation.

Authors:  Beat Vögeli
Journal:  J Biomol NMR       Date:  2011-06-03       Impact factor: 2.835

5.  Alternative E.COSY techniques for the measurement of 3J(C (i) (') (-1),C (i) (beta) ) and (3) J(H (i) (N) ,C (i) (beta) ) coupling constants in proteins.

Authors:  F Löhr; H Rüterjans
Journal:  J Biomol NMR       Date:  1999-03       Impact factor: 2.835

6.  An intraresidual i(HCA)CO(CA)NH experiment for the assignment of main-chain resonances in 15N, 13C labeled proteins.

Authors:  Sampo Mäntylahti; Helena Tossavainen; Maarit Hellman; Perttu Permi
Journal:  J Biomol NMR       Date:  2009-11       Impact factor: 2.835

7.  Compiled data set of exact NOE distance limits, residual dipolar couplings and scalar couplings for the protein GB3.

Authors:  Beat Vögeli; Simon Olsson; Roland Riek; Peter Güntert
Journal:  Data Brief       Date:  2015-09-04
  7 in total

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