Literature DB >> 19768387

An intraresidual i(HCA)CO(CA)NH experiment for the assignment of main-chain resonances in 15N, 13C labeled proteins.

Sampo Mäntylahti1, Helena Tossavainen, Maarit Hellman, Perttu Permi.   

Abstract

An improved pulse sequence, intraresidual i(HCA)CO(CA)NH, is described for establishing solely (13)C'(i), (15)N(i), (1)HN(i) connectivities in uniformly 15N/13C-labeled proteins. In comparison to the "out-and-back" style intra-HN(CA)CO experiment, the new pulse sequence offers at least two-fold higher experimental resolution in the (13)C' dimension and on average 1.6 times higher sensitivity especially for residues in alpha-helices. Performance of the new experiment was tested on a small globular protein ubiquitin and an intrinsically unfolded 110-residue cancer/testis antigen CT16/PAGE5. Use of intraresidual i(HCA)CO(CA)NH experiment in combination with the established HNCO experiment was crucial for the assignment of highly disordered CT16.

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Year:  2009        PMID： 19768387     DOI： 10.1007/s10858-009-9373-4

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  21 in total

Review 1.  Nuclear magnetic resonance methods for elucidation of structure and dynamics in disordered states.

Authors:  H J Dyson; P E Wright
Journal:  Methods Enzymol       Date:  2001       Impact factor: 1.600

2.  Use of the carbonyl chemical shift to relieve degeneracies in triple-resonance assignment experiments.

Authors:  E W Sayers; D A Torchia
Journal:  J Magn Reson       Date:  2001-12       Impact factor: 2.229

3.  A novel approach for the sequential backbone assignment of larger proteins: selective intra-HNCA and DQ-HNCA.

Authors:  Daniel Nietlispach; Yutaka Ito; Ernest D Laue
Journal:  J Am Chem Soc       Date:  2002-09-18       Impact factor: 15.419

4.  Optimized pathway selection in intraresidual triple-resonance experiments.

Authors:  Helena Tossavainen; Perttu Permi
Journal:  J Magn Reson       Date:  2004-10       Impact factor: 2.229

5.  Sensitivity enhancement in (HCA)CONH experiments.

Authors:  R Folmer; G Otting
Journal:  J Biomol NMR       Date:  2000-03       Impact factor: 2.835

6.  Rapid assignment of protein side chain resonances using projection-reconstruction of (4,3)D HC(CCO)NH and intra-HC(C)NH experiments.

Authors:  Ling Jiang; Brian E Coggins; Pei Zhou
Journal:  J Magn Reson       Date:  2005-07       Impact factor: 2.229

7.  Heteronuclear NMR studies of the combined Src homology domains 2 and 3 of pp60 c-Src: effects of phosphopeptide binding.

Authors:  M Tessari; L N Gentile; S J Taylor; D I Shalloway; L K Nicholson; G W Vuister
Journal:  Biochemistry       Date:  1997-11-25       Impact factor: 3.162

8.  Chemical shift dispersion and secondary structure prediction in unfolded and partly folded proteins.

Authors:  J Yao; H J Dyson; P E Wright
Journal:  FEBS Lett       Date:  1997-12-15       Impact factor: 4.124

9.  Measurement of 15N-13C J couplings in staphylococcal nuclease.

Authors:  F Delaglio; D A Torchia; A Bax
Journal:  J Biomol NMR       Date:  1991-11       Impact factor: 2.835

10.  Measurement of three-bond, 13C'-13C beta J couplings in human ubiquitin by a triple resonance, E. COSY-type NMR technique.

Authors:  J S Hu; A Bax
Journal:  J Biomol NMR       Date:  1998-02       Impact factor: 2.835
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  9 in total

1.  HA-detected experiments for the backbone assignment of intrinsically disordered proteins.

Authors:  Sampo Mäntylahti; Olli Aitio; Maarit Hellman; Perttu Permi
Journal:  J Biomol NMR       Date:  2010-05-01       Impact factor: 2.835

2.  Bridge over troubled proline: assignment of intrinsically disordered proteins using (HCA)CON(CAN)H and (HCA)N(CA)CO(N)H experiments concomitantly with HNCO and i(HCA)CO(CA)NH.

Authors:  Maarit Hellman; Henni Piirainen; Veli-Pekka Jaakola; Perttu Permi
Journal:  J Biomol NMR       Date:  2013-12-18       Impact factor: 2.835

3.  Extension of the HA-detection based approach: (HCA)CON(CA)H and (HCA)NCO(CA)H experiments for the main-chain assignment of intrinsically disordered proteins.

Authors:  Sampo Mäntylahti; Maarit Hellman; Perttu Permi
Journal:  J Biomol NMR       Date:  2011-01-25       Impact factor: 2.835

4.  Human adenosine A2A receptor binds calmodulin with high affinity in a calcium-dependent manner.

Authors:  Henni Piirainen; Maarit Hellman; Helena Tossavainen; Perttu Permi; Petri Kursula; Veli-Pekka Jaakola
Journal:  Biophys J       Date:  2015-02-17       Impact factor: 4.033

5.  The Disordered EZH2 Loop: Atomic Level Characterization by 1HN- and 1Hα-Detected NMR Approaches, Interaction with the Long Noncoding HOTAIR RNA.

Authors:  Csenge Lilla Szabó; Beáta Szabó; Fanni Sebák; Wolfgang Bermel; Agnes Tantos; Andrea Bodor
Journal:  Int J Mol Sci       Date:  2022-05-30       Impact factor: 6.208

6.  Characterization of intrinsically disordered prostate associated gene (PAGE5) at single residue resolution by NMR spectroscopy.

Authors:  Maarit Hellman; Helena Tossavainen; Pekka Rappu; Jyrki Heino; Perttu Permi
Journal:  PLoS One       Date:  2011-11-02       Impact factor: 3.240

7.  The Interaction Mechanism of Intrinsically Disordered PP2A Inhibitor Proteins ARPP-16 and ARPP-19 With PP2A.

Authors:  Chandan Thapa; Pekka Roivas; Tatu Haataja; Perttu Permi; Ulla Pentikäinen
Journal:  Front Mol Biosci       Date:  2021-03-26

8.  1H, 13C and 15N NMR chemical shift assignments of cAMP-regulated phosphoprotein-19 and -16 (ARPP-19 and ARPP-16).

Authors:  Chandan J Thapa; Tatu Haataja; Ulla Pentikäinen; Perttu Permi
Journal:  Biomol NMR Assign       Date:  2020-05-28       Impact factor: 0.746

9.  HACANCOi: a new Hα-detected experiment for backbone resonance assignment of intrinsically disordered proteins.

Authors:  Mikael Karjalainen; Helena Tossavainen; Maarit Hellman; Perttu Permi
Journal:  J Biomol NMR       Date:  2020-10-28       Impact factor: 2.835
  9 in total

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