Literature DB >> 96731

Beta-lactamases: determination of their isoelectric points.

M Barthelemy, M Guionie, R Labia.   

Abstract

Important discrepancies in isoelectric points (pI) of beta-lactamases were observed depending on the experimental procedure used for their determination: isoelectric focusing (IEF) in sucrose density gradients or analytical IEF in thin-layer polyacrylamide gels (PA). The variations, negligable in the case of TEM-like beta-lactamases, appeared to be important in the case of cephalosporinases and are related to an artifact which appears in PA-IEF. This has been clearly shown with beta-lactamase preparations from the following bacterial strains: Pseudomonas aeruginosa NCTC 8203 (pI = 8.7), P. aeruginosa NCTC 10701 (pI = 9.4), and Proteus morganii NCTC 235 (pI = 8.3). The data previously obtained by PA-IEF were much lower.

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Year:  1978        PMID： 96731      PMCID： PMC352312          DOI： 10.1128/AAC.13.4.695

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  8 in total

1.  [Multiplicity of beta lactamases: a problem of isoenzymes].

Authors:  R Labia; M Barthélémy; J M Masson
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1976-11-29

2.  [Kinetic study of 2 beta lactamases responsible for the same phenotype].

Authors:  R Labia; F Le Goffic; J Andrillon
Journal:  Biochimie       Date:  1974       Impact factor: 4.079

3.  Biochemical properties of a cephalosporin beta-lactamase from Pseudomonas aeruginosa.

Authors:  S Yaginuma; T Sawai; H Ono; S Yamagishi; S Mitsuhashi
Journal:  Jpn J Microbiol       Date:  1973-03

4.  The use of analytical isoelectric focusing for detection and identification of beta-lactamases.

Authors:  A Mathew; A M Harris; M J Marshall; G W Ross
Journal:  J Gen Microbiol       Date:  1975-05

5.  [Constitutive cephalosporinases from "Escherichia coli" (author's transl)].

Authors:  R Labia; G Brunet; M Guionie; A Philippon; M Heitz; J S Pitton
Journal:  Ann Microbiol (Paris)       Date:  1976 Nov-Dec

Review 6.  The beta-lactamases of gram-negative bacteria and their role in resistance to beta-lactam antibiotics.

Authors:  R B Sykes; M Matthew
Journal:  J Antimicrob Chemother       Date:  1976-06       Impact factor: 5.790

7.  Beta-lactamases produced by a Pseudomonas aeruginosa strain highly resistant to carbenicillin.

Authors:  R Labia; M Guionie; J M Masson; A Philippon; M Barthelemy
Journal:  Antimicrob Agents Chemother       Date:  1977-05       Impact factor: 5.191

8.  [Kinetics of a new cephalosporinase from Pseudomonas aeruginosa].

Authors:  R Labia; C Fabre; M Guionie
Journal:  Biochimie       Date:  1976       Impact factor: 4.079
  8 in total
  23 in total

1.  Cloning and sequence of the gene encoding a novel cefotaxime-hydrolyzing beta-lactamase (CTX-M-9) from Escherichia coli in Spain.

Authors:  M Sabaté; R Tarragó; F Navarro; E Miró; C Vergés; J Barbé; G Prats
Journal:  Antimicrob Agents Chemother       Date:  2000-07       Impact factor: 5.191

2.  Salmonella enterica serovar virchow with CTX-M-like beta-lactamase in Spain.

Authors:  E Simarro; F Navarro; J Ruiz; E Miró; J Gómez; B Mirelis
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

3.  Molecular epidemiology of a nosocomial outbreak due to SHV-4-producing strains of Citrobacter diversus.

Authors:  Z El Harrif-Heraud; C Arpin; S Benliman; C Quentin
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

4.  Postneurosurgical meningitis due to Proteus penneri with selection of a ceftriaxone-resistant isolate: analysis of chromosomal class A beta-lactamase HugA and its LysR-type regulatory protein HugR.

Authors:  Nadia Liassine; Stéphanie Madec; Béatrice Ninet; Catherine Metral; Martine Fouchereau-Peron; Roger Labia; Raymond Auckenthaler
Journal:  Antimicrob Agents Chemother       Date:  2002-01       Impact factor: 5.191

5.  Study of Pseudomonas aeruginosa serotype O12 isolates with a common antibiotic susceptibility pattern.

Authors:  A Talarmin; P Dubrous; P Gérome; Y Buisson
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1996-06       Impact factor: 3.267

6.  Discriminatory detection of inhibitor-resistant beta-lactamases in Escherichia coli by single-strand conformation polymorphism-PCR.

Authors:  V Speldooren; B Heym; R Labia; M H Nicolas-Chanoine
Journal:  Antimicrob Agents Chemother       Date:  1998-04       Impact factor: 5.191

7.  Novel, plasmid-encoded, TEM-derived extended-spectrum beta-lactamase in Klebsiella pneumoniae conferring higher resistance to aztreonam than to extended-spectrum cephalosporins.

Authors:  G Arlet; M Rouveau; G Fournier; P H Lagrange; A Philippon
Journal:  Antimicrob Agents Chemother       Date:  1993-09       Impact factor: 5.191

8.  Genetic and biochemical properties of AER-1, a novel carbenicillin-hydrolyzing beta-lactamase from Aeromonas hydrophila.

Authors:  R W Hedges; A A Medeiros; M Cohenford; G A Jacoby
Journal:  Antimicrob Agents Chemother       Date:  1985-04       Impact factor: 5.191

9.  Molecular epidemiology of Escherichia coli producing extended-spectrum beta-lactamases isolated in Rome, Italy.

Authors:  Alessandra Carattoli; Aurora García-Fernández; Paola Varesi; Daniela Fortini; Serena Gerardi; Adriano Penni; Carlo Mancini; Alessandra Giordano
Journal:  J Clin Microbiol       Date:  2007-10-24       Impact factor: 5.948

10.  Molecular characterization of a TEM-21 beta-lactamase in a clinical isolate of Morganella morganii.

Authors:  F Tessier; C Arpin; A Allery; C Quentin
Journal:  Antimicrob Agents Chemother       Date:  1998-08       Impact factor: 5.191
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