Literature DB >> 9666028

Competitive PCR for quantification of BM5d proviral DNA in mice with AIDS.

A Casabianca1, G Vallanti, M Magnani.   

Abstract

Murine AIDS in C57BL/6 mice is caused by a unique mixture of murine leukemia viruses. We report the use of a competitive PCR to detect and quantitate BM5d proviral DNA. This assay allowed discrimination among endogenous wild-type murine retroviruses and BM5d sequences. Furthermore, the method was subsequently used to evaluate the amount of BM5d in infected mice and in infected AZT (zidovudine)-treated mice, providing an effective way to quantitatively evaluate drug efficacy in the murine AIDS model.

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Year:  1998        PMID： 9666028      PMCID： PMC105054     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

Review 1.  Retrovirus-induced immunodeficiency in the mouse: MAIDS as a model for AIDS.

Authors:  H C Morse; S K Chattopadhyay; M Makino; T N Fredrickson; A W Hügin; J W Hartley
Journal:  AIDS       Date:  1992-07       Impact factor: 4.177

2.  Absolute quantitation of viremia in human immunodeficiency virus infection by competitive reverse transcription and polymerase chain reaction.

Authors:  S Menzo; P Bagnarelli; M Giacca; A Manzin; P E Varaldo; M Clementi
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

3.  Characteristics and contributions of defective, ecotropic, and mink cell focus-inducing viruses involved in a retrovirus-induced immunodeficiency syndrome of mice.

Authors:  S K Chattopadhyay; D N Sengupta; T N Fredrickson; H C Morse; J W Hartley
Journal:  J Virol       Date:  1991-08       Impact factor: 5.103

4.  Experiment and discussion on leukaemogenesis by cell-free extracts of radiation-induced leukaemia in mice.

Authors:  R LATARJET; J F DUPLAN
Journal:  Int J Radiat Biol Relat Stud Phys Chem Med       Date:  1962-08

5.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

Review 6.  Murine acquired immunodeficiency syndrome (MAIDS): an animal model to study the AIDS pathogenesis.

Authors:  P Jolicoeur
Journal:  FASEB J       Date:  1991-07       Impact factor: 5.191

7.  A p12 gag gene homologue is present in the mouse genome.

Authors:  A Casabianca; M Magnani
Journal:  Biochem Mol Biol Int       Date:  1994-03

8.  Quantitation of HIV-1 proviral DNA relative to cellular DNA by the polymerase chain reaction.

Authors:  D E Kellogg; J J Sninsky; S Kwok
Journal:  Anal Biochem       Date:  1990-09       Impact factor: 3.365

9.  An internally controlled virion PCR for the measurement of HIV-1 RNA in plasma.

Authors:  V Natarajan; R J Plishka; E W Scott; H C Lane; N P Salzman
Journal:  PCR Methods Appl       Date:  1994-06

10.  Molecular cloning and characterization of a murine AIDS virus-related endogenous transcript expressed in C57BL/6 mice.

Authors:  Y Kubo; Y Nakagawa; K Kakimi; H Matsui; K Higo; L Wang; H Kobayashi; T Hirama; A Ishimoto
Journal:  J Gen Virol       Date:  1994-04       Impact factor: 3.891
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  1 in total

1.  Development of a real-time PCR assay using SYBR Green I for provirus load quantification in a murine model of AIDS.

Authors:  Anna Casabianca; Chiara Orlandi; Alessandra Fraternale; Mauro Magnani
Journal:  J Clin Microbiol       Date:  2004-09       Impact factor: 5.948
  1 in total

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