Literature DB >> 15365045

Development of a real-time PCR assay using SYBR Green I for provirus load quantification in a murine model of AIDS.

Anna Casabianca1, Chiara Orlandi, Alessandra Fraternale, Mauro Magnani.   

Abstract

A real-time PCR assay using SYBR Green I for quantification of provirus load in a murine model of AIDS (i.e., LP-BM5 infection) was developed and validated. In this method, data are normalized against the 18S rRNA gene. The method has a dynamic range of 8 logs and a sensitivity of one copy.

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Year:  2004        PMID： 15365045      PMCID： PMC516357          DOI： 10.1128/JCM.42.9.4361-4364.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

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Authors:  T Pierson; J McArthur; R F Siliciano
Journal:  Annu Rev Immunol       Date:  2000       Impact factor: 28.527

2.  Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR.

Authors:  T D Schmittgen; B A Zakrajsek
Journal:  J Biochem Biophys Methods       Date:  2000-11-20

3.  Macrophage protection by addition of glutathione (GSH)-loaded erythrocytes to AZT and DDI in a murine AIDS model.

Authors:  A Fraternale; A Casabianca; C Orlandi; A Cerasi; L Chiarantini; G Brandi; M Magnani
Journal:  Antiviral Res       Date:  2002-12       Impact factor: 5.970

4.  Role of macrophage protection in the development of murine AIDS.

Authors:  A Fraternale; A Tonelli; A Casabianca; G Vallanti; L Chiarantini; G F Schiavano; U Benatti; A De Flora; M Magnani
Journal:  J Acquir Immune Defic Syndr       Date:  1999-06-01       Impact factor: 3.731

5.  New drug combinations for the treatment of murine AIDS and macrophage protection.

Authors:  A Fraternale; A Casabianca; A Tonelli; L Chiarantini; G Brandi; M Magnani
Journal:  Eur J Clin Invest       Date:  2001-03       Impact factor: 4.686

6.  Calibrated real-time PCR assay for quantitation of human herpesvirus 8 DNA in biological fluids.

Authors:  Francesco Broccolo; Giuseppe Locatelli; Loredana Sarmati; Sara Piergiovanni; Fabrizio Veglia; Massimo Andreoni; Stefano Buttò; Barbara Ensoli; Paolo Lusso; Mauro S Malnati
Journal:  J Clin Microbiol       Date:  2002-12       Impact factor: 5.948

7.  Zidovudine (AZT) reduces virus titer, retards immune dysfunction, and prolongs survival in the LP-BM5 murine induced immunodeficiency model.

Authors:  T Basham; C D Rios; T Holdener; T C Merigan
Journal:  J Infect Dis       Date:  1990-05       Impact factor: 5.226

8.  Rational design of drugs that induce human immunodeficiency virus replication.

Authors:  Dean H Hamer; Sven Bocklandt; Louise McHugh; Tae-Wook Chun; Peter M Blumberg; Dina M Sigano; Victor E Marquez
Journal:  J Virol       Date:  2003-10       Impact factor: 5.103

9.  Repeated cycles of alternate administration of fludarabine and Zidovudine plus Didanosine inhibits murine AIDS and reduces proviral DNA content in lymph nodes to undetectable levels.

Authors:  Alessandra Fraternale; Anna Casabianca; Chiara Orlandi; Laura Chiarantini; Giorgio Brandi; Guido Silvestri; Mauro Magnani
Journal:  Virology       Date:  2002-10-25       Impact factor: 3.616

10.  A new one-step RT-PCR method for virus quantitation in murine AIDS.

Authors:  Anna Casabianca; Chiara Orlandi; Alessandra Fraternale; Mauro Magnani
Journal:  J Virol Methods       Date:  2003-06-09       Impact factor: 2.014
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  5 in total

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Authors:  Christine L Clouser; Steven E Patterson; Louis M Mansky
Journal:  J Virol       Date:  2010-07-07       Impact factor: 5.103

2.  Activity of a novel combined antiretroviral therapy of gemcitabine and decitabine in a mouse model for HIV-1.

Authors:  Christine L Clouser; Colleen M Holtz; Mary Mullett; Daune L Crankshaw; Jacquie E Briggs; M Gerard O'Sullivan; Steven E Patterson; Louis M Mansky
Journal:  Antimicrob Agents Chemother       Date:  2012-01-23       Impact factor: 5.191

3.  Relationships of ESR1 and XBP1 expression in human breast carcinoma and stromal cells isolated by laser capture microdissection compared to intact breast cancer tissue.

Authors:  Sarah A Andres; James L Wittliff
Journal:  Endocrine       Date:  2011-08-21       Impact factor: 3.633

4.  Glutathione Depletion Is Linked with Th2 Polarization in Mice with a Retrovirus-Induced Immunodeficiency Syndrome, Murine AIDS: Role of Proglutathione Molecules as Immunotherapeutics.

Authors:  Serena Brundu; Linda Palma; Giusi Giada Picceri; Daniela Ligi; Chiara Orlandi; Luca Galluzzi; Laura Chiarantini; Anna Casabianca; Giuditta Fiorella Schiavano; Martina Santi; Ferdinando Mannello; Kathy Green; Michaël Smietana; Mauro Magnani; Alessandra Fraternale
Journal:  J Virol       Date:  2016-07-27       Impact factor: 5.103

5.  Analysis of the ex vivo and in vivo antiretroviral activity of gemcitabine.

Authors:  Christine L Clouser; Colleen M Holtz; Mary Mullett; Duane L Crankshaw; Jacquie E Briggs; Jay Chauhan; Ilze Matise VanHoutan; Steven E Patterson; Louis M Mansky
Journal:  PLoS One       Date:  2011-01-14       Impact factor: 3.240
  5 in total

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