Literature DB >> 9650979

Effects of various handling and storage conditions on stability of Treponema pallidum DNA in cerebrospinal fluid.

A V Villanueva1, R P Podzorski, M P Reyes.   

Abstract

Treponema pallidum DNA from even small numbers of organisms was detectable in cerebrospinal fluid (CSF) stored at room temperature or at 4 degrees C for several hours and in CSF subjected to three freeze-thaw cycles. These results suggest that negative PCR results for T. pallidum from patients diagnosed with T. pallidum invasion of the central nervous system are probably not due to the loss of target DNA prior to testing.

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Year:  1998        PMID： 9650979      PMCID： PMC104996     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

1.  Detection of Treponema pallidum in early syphilis by DNA amplification.

Authors:  K Wicher; G T Noordhoek; F Abbruscato; V Wicher
Journal:  J Clin Microbiol       Date:  1992-02       Impact factor: 5.948

2.  Diagnosis of neurosyphilis in patients infected with human immunodeficiency virus type 1.

Authors:  C M Marra; D W Gary; J Kuypers; M A Jacobson
Journal:  J Infect Dis       Date:  1996-07       Impact factor: 5.226

3.  Detection of treponemal DNA in the CSF of patients with syphilis and HIV infection using the polymerase chain reaction.

Authors:  P E Hay; J R Clarke; D Taylor-Robinson; D Goldmeier
Journal:  Genitourin Med       Date:  1990-12

4.  A randomized trial of enhanced therapy for early syphilis in patients with and without human immunodeficiency virus infection. The Syphilis and HIV Study Group.

Authors:  R T Rolfs; M R Joesoef; E F Hendershot; A M Rompalo; M H Augenbraun; M Chiu; G Bolan; S C Johnson; P French; E Steen; J D Radolf; S Larsen
Journal:  N Engl J Med       Date:  1997-07-31       Impact factor: 91.245

5.  Detection of Treponema pallidum by polymerase chain reaction in the cerebrospinal fluid of syphilis patients.

Authors:  K Y Chung; M G Lee; J B Lee
Journal:  Yonsei Med J       Date:  1994-06       Impact factor: 2.759

6.  Use of polymerase chain reaction and rabbit infectivity testing to detect Treponema pallidum in amniotic fluid, fetal and neonatal sera, and cerebrospinal fluid.

Authors:  E Grimprel; P J Sanchez; G D Wendel; J M Burstain; G H McCracken; J D Radolf; M V Norgard
Journal:  J Clin Microbiol       Date:  1991-08       Impact factor: 5.948

7.  Syphilis tests in diagnostic and therapeutic decision making.

Authors:  G Hart
Journal:  Ann Intern Med       Date:  1986-03       Impact factor: 25.391

8.  Detection by polymerase chain reaction of Treponema pallidum DNA in cerebrospinal fluid from neurosyphilis patients before and after antibiotic treatment.

Authors:  G T Noordhoek; E C Wolters; M E de Jonge; J D van Embden
Journal:  J Clin Microbiol       Date:  1991-09       Impact factor: 5.948

9.  Evaluation of molecular methodologies and rabbit infectivity testing for the diagnosis of congenital syphilis and neonatal central nervous system invasion by Treponema pallidum.

Authors:  P J Sánchez; G D Wendel; E Grimprel; M Goldberg; M Hall; O Arencibia-Mireles; J D Radolf; M V Norgard
Journal:  J Infect Dis       Date:  1993-01       Impact factor: 5.226

10.  Sensitive detection of Treponema pallidum by using the polymerase chain reaction.

Authors:  J M Burstain; E Grimprel; S A Lukehart; M V Norgard; J D Radolf
Journal:  J Clin Microbiol       Date:  1991-01       Impact factor: 5.948
  10 in total
  6 in total

Review 1.  PCR in diagnosis of infection: detection of bacteria in cerebrospinal fluids.

Authors:  Yoshimasa Yamamoto
Journal:  Clin Diagn Lab Immunol       Date:  2002-05

2.  Impact of long-term storage on stability of standard DNA for nucleic acid-based methods.

Authors:  Barbara Röder; Karin Frühwirth; Claus Vogl; Martin Wagner; Peter Rossmanith
Journal:  J Clin Microbiol       Date:  2010-09-01       Impact factor: 5.948

3.  Short-term stability of pathogen-specific nucleic acid targets in clinical samples.

Authors:  Mohammad R Hasan; Rusung Tan; Ghada N Al-Rawahi; Eva Thomas; Peter Tilley
Journal:  J Clin Microbiol       Date:  2012-10-10       Impact factor: 5.948

4.  Importance of sample preparation for molecular diagnosis of lyme borreliosis from urine.

Authors:  A R Bergmann; B L Schmidt; A-M Derler; E Aberer
Journal:  J Clin Microbiol       Date:  2002-12       Impact factor: 5.948

5.  Treponemal antibody in CSF and cellular immunity in peripheral blood of syphilitic patients with persisting positive rapid plasma regain.

Authors:  Wei-Qiang He; Huan-Li Wang; Dao-Qing Zhong; Lu-Yang Lin; Xiao-Shan Qiu; Ri-Dong Yang
Journal:  Int J Clin Exp Pathol       Date:  2015-05-01

6.  Evidence of rickettsiae in Danish patients tested for Lyme neuroborreliosis: a retrospective study of archival samples.

Authors:  Lukas Frans Ocias; Ram Benny Dessau; Anne-Mette Lebech; Charlotte Sværke Jørgensen; Randi Føns Petersen; Karen Angeliki Krogfelt
Journal:  BMC Infect Dis       Date:  2018-07-11       Impact factor: 3.090
  6 in total

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