Literature DB >> 1537923

Detection of Treponema pallidum in early syphilis by DNA amplification.

K Wicher1, G T Noordhoek, F Abbruscato, V Wicher.   

Abstract

By using experimentally infected rabbits as a model for early syphilis, the applicability of in vitro DNA amplification was explored for detection of Treponema pallidum. It was determined that whole blood in heparin or EDTA (but not serum), lesion exudate, and punch biopsy as well as swabs of lesions are useful specimens for examination by the polymerase chain reaction. Swabs do not require special diluents, and the specimens, whether kept at room temperature or frozen, are well suited for use in the polymerase chain reaction.

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Year:  1992        PMID： 1537923      PMCID： PMC265085          DOI： 10.1128/jcm.30.2.497-500.1992

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

Review 1.  New molecular techniques for microbial epidemiology and the diagnosis of infectious diseases.

Authors:  B I Eisenstein
Journal:  J Infect Dis       Date:  1990-04       Impact factor: 5.226

2.  Rapid and simple method for purification of nucleic acids.

Authors:  R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

3.  Use of the polymerase chain reaction to detect DNA sequences specific to pathogenic treponemes in cerebrospinal fluid.

Authors:  P E Hay; J R Clarke; R A Strugnell; D Taylor-Robinson; D Goldmeier
Journal:  FEMS Microbiol Lett       Date:  1990-03-15       Impact factor: 2.742

4.  Yaws in West Sumatra, Indonesia: clinical manifestations, serological findings and characterisation of new Treponema isolates by DNA probes.

Authors:  G T Noordhoek; H J Engelkens; J Judanarso; J van der Stek; G N Aelbers; J J van der Sluis; J D van Embden; E Stolz
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1991-01       Impact factor: 3.267

5.  Polymerase chain reaction and synthetic DNA probes: a means of distinguishing the causative agents of syphilis and yaws?

Authors:  G T Noordhoek; B Wieles; J J van der Sluis; J D van Embden
Journal:  Infect Immun       Date:  1990-06       Impact factor: 3.441

6.  Detection by polymerase chain reaction of Treponema pallidum DNA in cerebrospinal fluid from neurosyphilis patients before and after antibiotic treatment.

Authors:  G T Noordhoek; E C Wolters; M E de Jonge; J D van Embden
Journal:  J Clin Microbiol       Date:  1991-09       Impact factor: 5.948

7.  Pathogen-related spirochetes identified within gingival tissue from patients with acute necrotizing ulcerative gingivitis.

Authors:  G R Riviere; K S Weisz; L G Simonson; S A Lukehart
Journal:  Infect Immun       Date:  1991-08       Impact factor: 3.441

8.  Inhibition of human immunodeficiency virus gene amplification by heparin.

Authors:  M Holodniy; S Kim; D Katzenstein; M Konrad; E Groves; T C Merigan
Journal:  J Clin Microbiol       Date:  1991-04       Impact factor: 5.948

9.  Studies of rabbit testes infected with Treponema pallidum. I Immunopathology.

Authors:  K Wicher; V Wicher; S M Nakeeb; S Dubiski
Journal:  Br J Vener Dis       Date:  1983-12

10.  Sensitive detection of Treponema pallidum by using the polymerase chain reaction.

Authors:  J M Burstain; E Grimprel; S A Lukehart; M V Norgard; J D Radolf
Journal:  J Clin Microbiol       Date:  1991-01       Impact factor: 5.948
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  13 in total

1.  New tests for syphilis: rational design of a PCR method for detection of Treponema pallidum in clinical specimens using unique regions of the DNA polymerase I gene.

Authors:  H Liu; B Rodes; C Y Chen; B Steiner
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

2.  The laboratory diagnosis of syphilis.

Authors:  Sam Ratnam
Journal:  Can J Infect Dis Med Microbiol       Date:  2005-01       Impact factor: 2.471

3.  Evaluation of a PCR test for detection of treponema pallidum in swabs and blood.

Authors:  P A Grange; L Gressier; P L Dion; D Farhi; N Benhaddou; P Gerhardt; J P Morini; J Deleuze; C Pantoja; A Bianchi; F Lassau; M F Avril; M Janier; N Dupin
Journal:  J Clin Microbiol       Date:  2012-01-04       Impact factor: 5.948

4.  Identification of persistent infection in experimental syphilis by PCR.

Authors:  K Wicher; F Abbruscato; V Wicher; D N Collins; I Auger; H W Horowitz
Journal:  Infect Immun       Date:  1998-06       Impact factor: 3.441

5.  Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers.

Authors:  K A Orle; C A Gates; D H Martin; B A Body; J B Weiss
Journal:  J Clin Microbiol       Date:  1996-01       Impact factor: 5.948

6.  Detection of Treponema pallidum by a sensitive reverse transcriptase PCR.

Authors:  A Centurion-Lara; C Castro; J M Shaffer; W C Van Voorhis; C M Marra; S A Lukehart
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

7.  Treponema pallidum subsp. pertenue displays pathogenic properties different from those of T. pallidum subsp. pallidum.

Authors:  K Wicher; V Wicher; F Abbruscato; R E Baughn
Journal:  Infect Immun       Date:  2000-06       Impact factor: 3.441

8.  Target organs of infection in guinea pigs with acquired congenital syphilis.

Authors:  K Wicher; F Abbruscato; V Wicher; R Baughn; G T Noordhoek
Journal:  Infect Immun       Date:  1996-08       Impact factor: 3.441

Review 9.  Laboratory diagnosis and interpretation of tests for syphilis.

Authors:  S A Larsen; B M Steiner; A H Rudolph
Journal:  Clin Microbiol Rev       Date:  1995-01       Impact factor: 26.132

10.  Effects of various handling and storage conditions on stability of Treponema pallidum DNA in cerebrospinal fluid.

Authors:  A V Villanueva; R P Podzorski; M P Reyes
Journal:  J Clin Microbiol       Date:  1998-07       Impact factor: 5.948
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