Literature DB >> 20810770

Impact of long-term storage on stability of standard DNA for nucleic acid-based methods.

Barbara Röder1, Karin Frühwirth, Claus Vogl, Martin Wagner, Peter Rossmanith.   

Abstract

Real-time PCR is dependent upon a calibration function for quantification. While long-term storage of standards saves cost and time, solutions of DNA are prone to degradation. We present here the benchmark treatment for preservation of DNA standards, involving storage in 50% glycerol-double-distilled water, whereby a deviation of 0.2 threshold cycle (C(T)) values resulted after 100 days of storage.

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Year:  2010        PMID： 20810770      PMCID： PMC3020885          DOI： 10.1128/JCM.01230-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

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