Literature DB >> 23052319

Short-term stability of pathogen-specific nucleic acid targets in clinical samples.

Mohammad R Hasan1, Rusung Tan, Ghada N Al-Rawahi, Eva Thomas, Peter Tilley.   

Abstract

The stability of pathogen-specific DNA or RNA amplification targets in clinical samples following short-term storage at room temperature, 4°C, and -80°C was assessed by real-time PCR. In purified nucleic acid extracts, both DNA and RNA targets were stable for up to 30 days, irrespective of storage temperature. In unextracted samples, temperature-dependent loss of targets (P < 0.05) was observed in serum and cerebrospinal fluid specimens, while no changes were observed for EDTA blood samples.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 23052319      PMCID： PMC3502979          DOI： 10.1128/JCM.02659-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

Authors:  K J Livak; T D Schmittgen
Journal:  Methods       Date:  2001-12       Impact factor: 3.608

2.  Quantitative stability of DNA after extended storage of clinical specimens as determined by real-time PCR.

Authors:  Keith R Jerome; Meei-Li Huang; Anna Wald; Stacy Selke; Lawrence Corey
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

Review 3.  Molecular diagnostics in medical microbiology: yesterday, today and tomorrow.

Authors:  Alex van Belkum
Journal:  Curr Opin Pharmacol       Date:  2003-10       Impact factor: 5.547

Review 4.  Validation of laboratory-developed molecular assays for infectious diseases.

Authors:  Eileen M Burd
Journal:  Clin Microbiol Rev       Date:  2010-07       Impact factor: 26.132

5.  Stability of HCV, HIV-1 and HBV nucleic acids in plasma samples under long-term storage.

Authors:  Marta José; Rodrigo Gajardo; Juan I Jorquera
Journal:  Biologicals       Date:  2005-03       Impact factor: 1.856

6.  Effects of storage and type of blood collection tubes on hepatitis C virus level in whole blood samples.

Authors:  H H Kessler; E Stelzl; R B Raggam; J Haas; F Kirchmeir; K Hegenbarth; E Daghofer; B I Santner; E Marth; R E Stauber
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

7.  Assessment of hepatitis B virus DNA stability in serum by the Chiron Quantiplex branched-DNA assay.

Authors:  M Krajden; L Comanor; O Rifkin; A Grigoriew; J M Minor; G F Kapke
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948

8.  Stability of Trichomonas vaginalis DNA in urine specimens.

Authors:  Jessica Ingersoll; Tameka Bythwood; Deborah Abdul-Ali; Gina M Wingood; Ralph J Diclemente; Angela M Caliendo
Journal:  J Clin Microbiol       Date:  2008-03-12       Impact factor: 5.948

9.  Effects of various handling and storage conditions on stability of Treponema pallidum DNA in cerebrospinal fluid.

Authors:  A V Villanueva; R P Podzorski; M P Reyes
Journal:  J Clin Microbiol       Date:  1998-07       Impact factor: 5.948
  9 in total
  8 in total

1.  Consistent viral DNA quantification after prolonged storage at ambient temperature.

Authors:  Benjamin Zaniello; Meei-Li Huang; Anqi Cheng; Stacy Selke; Anna Wald; Keith R Jerome; Amalia S Magaret
Journal:  J Virol Methods       Date:  2015-11-22       Impact factor: 2.014

2.  Field Evaluation of Capillary Blood Samples as a Collection Specimen for the Rapid Diagnosis of Ebola Virus Infection During an Outbreak Emergency.

Authors:  Thomas Strecker; Bernadett Palyi; Heinz Ellerbrok; Sylvie Jonckheere; Hilde de Clerck; Joseph Akoi Bore; Martin Gabriel; Kilian Stoecker; Markus Eickmann; Michel van Herp; Pierre Formenty; Antonino Di Caro; Stephan Becker
Journal:  Clin Infect Dis       Date:  2015-05-19       Impact factor: 9.079

Review 3.  More Pathogenicity or Just More Pathogens?-On the Interpretation Problem of Multiple Pathogen Detections with Diagnostic Multiplex Assays.

Authors:  Andreas E Zautner; Uwe Groß; Matthias F Emele; Ralf M Hagen; Hagen Frickmann
Journal:  Front Microbiol       Date:  2017-06-29       Impact factor: 5.640

Review 4.  Metagenomics for neurological infections - expanding our imagination.

Authors:  Prashanth S Ramachandran; Michael R Wilson
Journal:  Nat Rev Neurol       Date:  2020-07-13       Impact factor: 42.937

5.  Analysis and modeling of coolants and coolers for specimen transportation.

Authors:  David E Lowe; Gerald Pellegrini; Elizabeth LeMasters; Andrew J Carter; Zachary P Weiner
Journal:  PLoS One       Date:  2020-04-17       Impact factor: 3.240

6.  Identification of Viruses in Cases of Pediatric Acute Encephalitis and Encephalopathy Using Next-Generation Sequencing.

Authors:  Jun-Ichi Kawada; Yusuke Okuno; Yuka Torii; Ryo Okada; Satoshi Hayano; Shotaro Ando; Yasuko Kamiya; Seiji Kojima; Yoshinori Ito
Journal:  Sci Rep       Date:  2016-09-14       Impact factor: 4.379

Review 7.  Molecular Diagnosis of Tuberculosis.

Authors:  Fariz Nurwidya; Diah Handayani; Erlina Burhan; Faisal Yunus
Journal:  Chonnam Med J       Date:  2018-01-25

8.  Anatomical distribution of Toxoplasma gondii in naturally and experimentally infected lambs.

Authors:  Myriam Thomas; Dominique Aubert; Sandie Escotte-Binet; Benoît Durand; Céline Robert; Régine Geers; Annie Alliot; Guillaume Belbis; Isabelle Villena; Radu Blaga
Journal:  Parasite       Date:  2022-02-01       Impact factor: 3.000
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.