Literature DB >> 9561796

Evaluation of charging on macromolecules in electron cryomicroscopy.

J Brink1, M B Sherman, J Berriman, W Chiu.   

Abstract

We describe procedures to assess charging of biological specimens under electron irradiation in an electron cryomicroscope. Charging can be observed by an expansion of the illuminating beam, blurring of electron diffraction patterns and by beam "footprints" on the specimen. Discharging can also be seen in the defocused electron diffraction mode. We investigated the influence of a variety of factors on the magnitude and visibility of charging. A reduction of charging is noticed when part of the adjacent carbon film is included in the irradiated specimen area.

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Year:  1998        PMID： 9561796     DOI： 10.1016/s0304-3991(97)00126-5

Source DB:  PubMed          Journal:  Ultramicroscopy        ISSN： 0304-3991            Impact factor:   2.689


  18 in total

1.  Automation of random conical tilt and orthogonal tilt data collection using feature-based correlation.

Authors:  Craig Yoshioka; James Pulokas; Denis Fellmann; Clinton S Potter; Ronald A Milligan; Bridget Carragher
Journal:  J Struct Biol       Date:  2007-04-20       Impact factor: 2.867

2.  Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction.

Authors:  Xing Zhang; Ethan Settembre; Chen Xu; Philip R Dormitzer; Richard Bellamy; Stephen C Harrison; Nikolaus Grigorieff
Journal:  Proc Natl Acad Sci U S A       Date:  2008-01-31       Impact factor: 11.205

Review 3.  Challenges and opportunities in cryo-EM single-particle analysis.

Authors:  Dmitry Lyumkis
Journal:  J Biol Chem       Date:  2019-02-25       Impact factor: 5.157

Review 4.  Electron cryomicroscopy of membrane proteins: specimen preparation for two-dimensional crystals and single particles.

Authors:  Ingeborg Schmidt-Krey; John L Rubinstein
Journal:  Micron       Date:  2010-07-16       Impact factor: 2.251

5.  The surface of evaporated carbon films is an insulating, high-bandgap material.

Authors:  David M Larson; Kenneth H Downing; Robert M Glaeser
Journal:  J Struct Biol       Date:  2011-02-19       Impact factor: 2.867

Review 6.  Conquer by cryo-EM without physically dividing.

Authors:  Gabriel C Lander; Robert M Glaeser
Journal:  Biochem Soc Trans       Date:  2021-11-01       Impact factor: 4.919

7.  Cryomesh: a new substrate for cryo-electron microscopy.

Authors:  Craig Yoshioka; Bridget Carragher; Clinton S Potter
Journal:  Microsc Microanal       Date:  2010-02       Impact factor: 4.127

Review 8.  The use of trehalose in the preparation of specimens for molecular electron microscopy.

Authors:  Po-Lin Chiu; Deborah F Kelly; Thomas Walz
Journal:  Micron       Date:  2011-06-25       Impact factor: 2.251

9.  Beam-induced motion of vitrified specimen on holey carbon film.

Authors:  Axel F Brilot; James Z Chen; Anchi Cheng; Junhua Pan; Stephen C Harrison; Clinton S Potter; Bridget Carragher; Richard Henderson; Nikolaus Grigorieff
Journal:  J Struct Biol       Date:  2012-02-16       Impact factor: 2.867

10.  A dose-rate effect in single-particle electron microscopy.

Authors:  James Z Chen; Carsten Sachse; Chen Xu; Thorsten Mielke; Christian M T Spahn; Nikolaus Grigorieff
Journal:  J Struct Biol       Date:  2007-10-01       Impact factor: 2.867
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