Literature DB >> 9335045

Applications of the green fluorescent protein in cell biology and biotechnology.

T Misteli1, D L Spector.   

Abstract

The recent emergence of an autofluorescent protein, the green fluorescent protein (GFP), has opened the door for the convenient use of intact living cells and organisms as experimental systems in fields ranging from cell biology to biomedicine. We present an overview of some of the major applications of GFP, namely its use in protein tagging and in monitoring gene expression as well as its potential in a variety of biological screens.

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Year:  1997        PMID: 9335045     DOI: 10.1038/nbt1097-961

Source DB:  PubMed          Journal:  Nat Biotechnol        ISSN: 1087-0156            Impact factor:   54.908


  63 in total

Review 1.  Stress and the cell nucleus: dynamics of gene expression and structural reorganization.

Authors:  C Jolly; R I Morimoto
Journal:  Gene Expr       Date:  1999

2.  Molecular basis for pH sensitivity and proton transfer in green fluorescent protein: protonation and conformational substates from electrostatic calculations.

Authors:  C Scharnagl; R Raupp-Kossmann; S F Fischer
Journal:  Biophys J       Date:  1999-10       Impact factor: 4.033

3.  One- and two-photon excited fluorescence lifetimes and anisotropy decays of green fluorescent proteins.

Authors:  A Volkmer; V Subramaniam; D J Birch; T M Jovin
Journal:  Biophys J       Date:  2000-03       Impact factor: 4.033

4.  Green fluorescent protein functions as a reporter for protein localization in Escherichia coli.

Authors:  B J Feilmeier; G Iseminger; D Schroeder; H Webber; G J Phillips
Journal:  J Bacteriol       Date:  2000-07       Impact factor: 3.490

5.  Quantitative imaging of pre-mRNA splicing factors in living cells.

Authors:  R Eils; D Gerlich; W Tvaruskó; D L Spector; T Misteli
Journal:  Mol Biol Cell       Date:  2000-02       Impact factor: 4.138

6.  Use of green fluorescent protein-conjugated beta-actin as a novel molecular marker for in vitro tumor cell chemotaxis assay.

Authors:  L Hodgson; W Qiu; C Dong; A J Henderson
Journal:  Biotechnol Prog       Date:  2000 Nov-Dec

7.  Visualization and molecular analysis of nuclear import of protein kinase CK2 subunits in living cells.

Authors:  V Martel; O Filhol; A Nueda; D Gerber; M J Benitez; C Cochet
Journal:  Mol Cell Biochem       Date:  2001-11       Impact factor: 3.396

Review 8.  Gene-marking studies of hematopoietic cells.

Authors:  C M Bollard; H E Heslop; M K Brenner
Journal:  Int J Hematol       Date:  2001-01       Impact factor: 2.490

9.  Shedding light on the dark and weakly fluorescent states of green fluorescent proteins.

Authors:  W Weber; V Helms; J A McCammon; P W Langhoff
Journal:  Proc Natl Acad Sci U S A       Date:  1999-05-25       Impact factor: 11.205

Review 10.  Imaging gene expression: principles and assays.

Authors:  S S Gambhir; J R Barrio; H R Herschman; M E Phelps
Journal:  J Nucl Cardiol       Date:  1999 Mar-Apr       Impact factor: 5.952

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