Literature DB >> 9501306

Rapid mapping of markers applying vectorette technology to YAC fragmentation allows easy assembly of a high-density STS bacterial clone contig spanning the markers D6S1260-D6S1918.

J D Shearman1, J J Pointon, A T Merryweather-Clarke, C Stone, S W Horsley, L Kearney, W M Rosenberg, K J Robson.   

Abstract

We have generated a detailed physical map of the 6p21.3/p22.1 boundary, using a combination of yeast artificial chromosome (YAC) fragmentation and high-resolution sequence tagged site (STS) content mapping. YACs from the CEPH, St. Louis, and ICRF libraries have been used to construct a 4.5-Mb contig spanning the markers D6S306 to D6S1571. YAC insert sizes were determined by pulsed field gel electrophoresis (PFGE). Chimerism of YACs was determined by fluorescent in situ hybridization (FISH), and their integrity was determined by fingerprinting with Alu-PCR. We have identified 10 new CA repeat loci in this region as well as over 50 novel STSs, several tRNA genes, a new histone H2B gene and the phospholipase D gene. Using these new markers, we have rapidly generated a bacterial clone contig of over 250 kb, spanning the markers D6S1260 to D6S1918 (WI-3111) with STSs spaced on average every 6 kb.

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Year:  1998        PMID： 9501306     DOI： 10.1007/s003359900729

Source DB:  PubMed          Journal:  Mamm Genome        ISSN： 0938-8990            Impact factor:   2.957


  26 in total

1.  Preparation of radiolabelled hybridization probes by STS labelling.

Authors:  M C Hirst; J H Bassett; A Roche; K E Davies
Journal:  Trends Genet       Date:  1992-01       Impact factor: 11.639

2.  Improved method for high efficiency transformation of intact yeast cells.

Authors:  D Gietz; A St Jean; R A Woods; R H Schiestl
Journal:  Nucleic Acids Res       Date:  1992-03-25       Impact factor: 16.971

3.  A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones.

Authors:  J Riley; R Butler; D Ogilvie; R Finniear; D Jenner; S Powell; R Anand; J C Smith; A F Markham
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

4.  A 1.1-Mb transcript map of the hereditary hemochromatosis locus.

Authors:  D A Ruddy; G S Kronmal; V K Lee; G A Mintier; L Quintana; R Domingo; N C Meyer; A Irrinki; E E McClelland; A Fullan; F A Mapa; T Moore; W Thomas; D B Loeb; C Harmon; Z Tsuchihashi; R K Wolff; R C Schatzman; J N Feder
Journal:  Genome Res       Date:  1997-05       Impact factor: 9.043

5.  Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources.

Authors:  D L Nelson; S A Ledbetter; L Corbo; M F Victoria; R Ramírez-Solis; T D Webster; D H Ledbetter; C T Caskey
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

6.  A comprehensive genetic map of the human genome based on 5,264 microsatellites.

Authors:  C Dib; S Fauré; C Fizames; D Samson; N Drouot; A Vignal; P Millasseau; S Marc; J Hazan; E Seboun; M Lathrop; G Gyapay; J Morissette; J Weissenbach
Journal:  Nature       Date:  1996-03-14       Impact factor: 49.962

7.  An STS-based map of the human genome.

Authors:  T J Hudson; L D Stein; S S Gerety; J Ma; A B Castle; J Silva; D K Slonim; R Baptista; L Kruglyak; S H Xu; X Hu; A M Colbert; C Rosenberg; M P Reeve-Daly; S Rozen; L Hui; X Wu; C Vestergaard; K M Wilson; J S Bae; S Maitra; S Ganiatsas; C A Evans; M M DeAngelis; K A Ingalls; R W Nahf; L T Horton; M O Anderson; A J Collymore; W Ye; V Kouyoumjian; I S Zemsteva; J Tam; R Devine; D F Courtney; M T Renaud; H Nguyen; T J O'Connor; C Fizames; S Fauré; G Gyapay; C Dib; J Morissette; J B Orlin; B W Birren; N Goodman; J Weissenbach; T L Hawkins; S Foote; D C Page; E S Lander
Journal:  Science       Date:  1995-12-22       Impact factor: 47.728

8.  Construction of cosmid libraries from flow-sorted human chromosomes 1, 6, 7, 11, 13, and 18 for reference library resources.

Authors:  D Nizetic; S Monard; B Young; F Cotter; G Zehetner; H Lehrach
Journal:  Mamm Genome       Date:  1994-12       Impact factor: 2.957

9.  Localization of the hemochromatosis gene close to D6S105.

Authors:  E C Jazwinska; S C Lee; S I Webb; J W Halliday; L W Powell
Journal:  Am J Hum Genet       Date:  1993-08       Impact factor: 11.025

10.  New polymorphic microsatellite markers place the haemochromatosis gene telomeric to D6S105.

Authors:  R Raha-Chowdhury; D J Bowen; C Stone; J J Pointon; J D Terwilliger; J D Shearman; K J Robson; A Bomford; M Worwood
Journal:  Hum Mol Genet       Date:  1995-10       Impact factor: 6.150
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