Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones.

Literature DB >> 2161516

A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones.

J Riley¹, R Butler, D Ogilvie, R Finniear, D Jenner, S Powell, R Anand, J C Smith, A F Markham.

Abstract

The recent development of yeast artificial chromosome (YAC) vectors has provided a system for cloning fragments that are over ten times larger than those that can be cloned in more established systems. We have developed a method for the rapid isolation of terminal sequences from YAC clones. The YAC clone is digested with a range of restriction enzymes, a common linker is ligated to the DNA fragments and terminal sequences are amplified using a vector specific primer and a linker specific primer. Sequence data derived from these terminal specific products can be used to design primers for a further round of screening to isolate overlapping clones. The method also provides a convenient method of generating Sequence Tagged Sites for the mapping of complex genomes.

Entities: Species

Mesh：

Substances：
DNA Restriction Enzymes

Year: 1990 PMID： 2161516 PMCID： PMC330815 DOI： 10.1093/nar/18.10.2887

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

13 in total

1. Toward a physical map of the genome of the nematode Caenorhabditis elegans.

Authors: A Coulson; J Sulston; S Brenner; J Karn
Journal: Proc Natl Acad Sci U S A Date: 1986-10 Impact factor: 11.205

2. A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.

Authors: R Anand; J H Riley; R Butler; J C Smith; A F Markham
Journal: Nucleic Acids Res Date: 1990-04-25 Impact factor: 16.971

3. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

Authors: D T Burke; G F Carle; M V Olson
Journal: Science Date: 1987-05-15 Impact factor: 47.728

4. A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences.

Authors: T Triglia; M G Peterson; D J Kemp
Journal: Nucleic Acids Res Date: 1988-08-25 Impact factor: 16.971

5. A common language for physical mapping of the human genome.

Authors: M Olson; L Hood; C Cantor; D Botstein
Journal: Science Date: 1989-09-29 Impact factor: 47.728

6. Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources.

Authors: D L Nelson; S A Ledbetter; L Corbo; M F Victoria; R Ramírez-Solis; T D Webster; D H Ledbetter; C T Caskey
Journal: Proc Natl Acad Sci U S A Date: 1989-09 Impact factor: 11.205

7. Genetic applications of an inverse polymerase chain reaction.

Authors: H Ochman; A S Gerber; D L Hartl
Journal: Genetics Date: 1988-11 Impact factor: 4.562

8. Genome linking with yeast artificial chromosomes.

Authors: A Coulson; R Waterston; J Kiff; J Sulston; Y Kohara
Journal: Nature Date: 1988-09-08 Impact factor: 49.962

9. Isolation of single-copy human genes from a library of yeast artificial chromosome clones.

Authors: B H Brownstein; G A Silverman; R D Little; D T Burke; S J Korsmeyer; D Schlessinger; M V Olson
Journal: Science Date: 1989-06-16 Impact factor: 47.728

10. Diagnosis of alpha 1-antitrypsin deficiency by enzymatic amplification of human genomic DNA and direct sequencing of polymerase chain reaction products.

Authors: C R Newton; N Kalsheker; A Graham; S Powell; A Gammack; J Riley; A F Markham
Journal: Nucleic Acids Res Date: 1988-09-12 Impact factor: 16.971

177 in total

1. High-resolution landmark framework for the sequence-ready mapping of Xq23-q26.1.

Authors: H E Steingruber; A Dunham; A J Coffey; S M Clegg; G R Howell; G L Maslen; C E Scott; R Gwilliam; P J Hunt; E C Sotheran; E J Huckle; S E Hunt; P Dhami; C Soderlund; M A Leversha; D R Bentley; M T Ross
Journal: Genome Res Date: 1999-08 Impact factor: 9.043

2. A high-resolution physical map of human chromosome 21p using yeast artificial chromosomes.

Authors: S Y Wang; M Cruts; J Del-Favero; Y Zhang; F Tissir; M C Potier; D Patterson; D Nizetic; A Bosch; H Chen; L Bennett; X Estivill; A Kessling; S E Antonarakis; C van Broeckhoven
Journal: Genome Res Date: 1999-11 Impact factor: 9.043

3. Organization of human and mouse skeletal myosin heavy chain gene clusters is highly conserved.

Authors: A Weiss; D McDonough; B Wertman; L Acakpo-Satchivi; K Montgomery; R Kucherlapati; L Leinwand; K Krauter
Journal: Proc Natl Acad Sci U S A Date: 1999-03-16 Impact factor: 11.205

4. High-resolution transcript map of the region spanning D12S1629 and D12S312 at chromosome 12q13: triple A syndrome-linked region.

Authors: H Lee; E Choi; Y Seomun; K Montgomery; A Huebner; E Lee; S Lau; C K Joo; R Kucherlapati; S J Yoon
Journal: Genome Res Date: 2000-10 Impact factor: 9.043

5. Rapid genome walking: a simplified oligo-cassette mediated polymerase chain reaction using a single genome-specific primer.

Authors: M Kilstrup; K N Kristiansen
Journal: Nucleic Acids Res Date: 2000-06-01 Impact factor: 16.971

9. Construction of a genomic DNA 'feature map' by sequencing from nested deletions: application to the HLA class I region.

Authors: B R Krishnan; I Jamry; D E Berg; C M Berg; D D Chaplin
Journal: Nucleic Acids Res Date: 1995-01-11 Impact factor: 16.971

10. Evidence that Spt10 and Spt21 of Saccharomyces cerevisiae play distinct roles in vivo and functionally interact with MCB-binding factor, SCB-binding factor and Snf1.

Authors: David Hess; Fred Winston
Journal: Genetics Date: 2005-03-02 Impact factor: 4.562