Literature DB >> 9464425

Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA.

J R Marchesi1, T Sato, A J Weightman, T A Martin, J C Fry, S J Hiom, D Dymock, W G Wade.   

Abstract

We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

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Year:  1998        PMID： 9464425      PMCID： PMC106123     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

1.  DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities.

Authors:  P A Rochelle; J C Fry; R J Parkes; A J Weightman
Journal:  FEMS Microbiol Lett       Date:  1992-12-15       Impact factor: 2.742

2.  Archaea in coastal marine environments.

Authors:  E F DeLong
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-15       Impact factor: 11.205

Review 3.  Bacterial evolution.

Authors:  C R Woese
Journal:  Microbiol Rev       Date:  1987-06

Review 4.  Phylogenetic identification and in situ detection of individual microbial cells without cultivation.

Authors:  R I Amann; W Ludwig; K H Schleifer
Journal:  Microbiol Rev       Date:  1995-03

5.  Phylogenetic analysis of the hyperthermophilic pink filament community in Octopus Spring, Yellowstone National Park.

Authors:  A L Reysenbach; G S Wickham; N R Pace
Journal:  Appl Environ Microbiol       Date:  1994-06       Impact factor: 4.792

6.  The oligonucleotide probe database.

Authors:  E W Alm; D B Oerther; N Larsen; D A Stahl; L Raskin
Journal:  Appl Environ Microbiol       Date:  1996-10       Impact factor: 4.792

7.  A computer analysis of primer and probe hybridization potential with bacterial small-subunit rRNA sequences.

Authors:  C F Brunk; E Avaniss-Aghajani; C A Brunk
Journal:  Appl Environ Microbiol       Date:  1996-03       Impact factor: 4.792

8.  Database on the structure of small ribosomal subunit RNA.

Authors:  Y Van de Peer; J Jansen; P De Rijk; R De Wachter
Journal:  Nucleic Acids Res       Date:  1997-01-01       Impact factor: 16.971

9.  Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.

Authors:  J Brosius; M L Palmer; P J Kennedy; H F Noller
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205
  9 in total
  324 in total

1.  Bacterial primary colonization and early succession on surfaces in marine waters as determined by amplified rRNA gene restriction analysis and sequence analysis of 16S rRNA genes.

Authors:  H Dang; C R Lovell
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

2.  Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization.

Authors:  F O Glöckner; B M Fuchs; R Amann
Journal:  Appl Environ Microbiol       Date:  1999-08       Impact factor: 4.792

3.  Rapid detection of sepsis complicating acute necrotizing pancreatitis using polymerase chain reaction.

Authors:  W Z Zhang; T Q Han; Y Q Tang; S D Zhang
Journal:  World J Gastroenterol       Date:  2001-04       Impact factor: 5.742

4.  Molecular identification of microorganisms from endodontic infections.

Authors:  H J Rolph; A Lennon; M P Riggio; W P Saunders; D MacKenzie; L Coldero; J Bagg
Journal:  J Clin Microbiol       Date:  2001-09       Impact factor: 5.948

5.  Rapid detection of Shigella species in environmental sewage by an immunocapture PCR with universal primers.

Authors:  Xuanxian Peng; Wen Luo; Jianying Zhang; Sanying Wang; Shengcai Lin
Journal:  Appl Environ Microbiol       Date:  2002-05       Impact factor: 4.792

6.  Soil type is the primary determinant of the composition of the total and active bacterial communities in arable soils.

Authors:  Martina S Girvan; Juliet Bullimore; Jules N Pretty; A Mark Osborn; Andrew S Ball
Journal:  Appl Environ Microbiol       Date:  2003-03       Impact factor: 4.792

7.  Laser MICROSAMPLING of soil microbial community.

Authors:  M V Gorlenko; E A Chutko; E S Churbanova; N V Minaev; K I Kachesov; L V Lysak; S A Evlashin; V S Cheptsov; A O Rybaltovskiy; V I Yusupov; V S Zhigarkov; G A Davydova; B N Chichkov; V N Bagratashvili
Journal:  J Biol Eng       Date:  2018-11-28       Impact factor: 4.355

8.  Prey range characterization, ribotyping, and diversity of soil and rhizosphere Bdellovibrio spp. isolated on phytopathogenic bacteria.

Authors:  E Jurkevitch; D Minz; B Ramati; G Barel
Journal:  Appl Environ Microbiol       Date:  2000-06       Impact factor: 4.792

9.  Role of bacterial adhesion in the microbial ecology of biofilms in cooling tower systems.

Authors:  Yang Liu; Wei Zhang; Tadas Sileika; Richard Warta; Nicholas P Cianciotto; Aaron Packman
Journal:  Biofouling       Date:  2009       Impact factor: 3.209

10.  Genomic Comparison Reveals Natural Occurrence of Clinically Relevant Multidrug-Resistant Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strains.

Authors:  Lin Teng; Shinyoung Lee; Amber Ginn; Sarah M Markland; Raies A Mir; Nicolas DiLorenzo; Christina Boucher; Mattia Prosperi; Judith Johnson; J Glenn Morris; Kwangcheol C Jeong
Journal:  Appl Environ Microbiol       Date:  2019-06-17       Impact factor: 4.792
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