Literature DB >> 1282488

DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities.

P A Rochelle1, J C Fry, R J Parkes, A J Weightman.   

Abstract

A protocol was devised which permitted the extraction of DNA from deep marine sediments up to 503 m below the sea floor. These sediments have been laid down over the last 3 million years. 16S rRNA gene sequences were amplified from the DNA by the polymerase chain reaction. The details of the successful extraction and polymerase chain reaction methodology varied between samples from different depths. This emphasizes the attention to detail required to allow the diversity of bacteria in these deep sediments to be studied.

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Year:  1992        PMID： 1282488     DOI： 10.1111/j.1574-6968.1992.tb14019.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  25 in total

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Authors:  D H Lee; Y G Zo; S J Kim
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6.  Phylogenetic analysis of the bacterial communities in marine sediments.

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9.  Analysis of subfossil molecular remains of purple sulfur bacteria in a lake sediment.

Authors:  M J Coolen; J Overmann
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