| Literature DB >> 9399538 |
M Bogard1, C Buffet-Janvresse, J F Cantaloube, P Biagini, G Duverlie, S Castelain, J Izopet, M Dubois, C Defer, I Lepot, J Coste, P Marcellin, M Martinot-Peignoux, P Halfon, V Gerolami, L Frangeul, J M Pawlotsky, F Roudot-Thoraval, E Dussaix, P Loiseau, N Ravera, P Lewin, J Lamoril, J Lerable, P Lebon.
Abstract
PCR is, to date, the only available tool for the detection of GB virus C (GBV-C) and hepatitis G virus (HGV) RNAs. Twenty-two French laboratories participated in a quality control study to assess the sensitivity and specificity of their procedures. The panel included 13 positive controls and 7 negative controls. The laboratories used either in-house PCR techniques adapted from the literature or partly standardized commercial tests. Three laboratories performed faultlessly with the entire panel. Most laboratories had excellent specificity (100% in 20 of 22 laboratories). Sensitivity was acceptable (85 to 100%) in 15 centers and insufficient (38 to 77%) in 7. As with nonstandardized in-house PCR, the commercial assays gave discrepant performances in different laboratories. These results suggest that laboratories willing to use PCR for detection of GBV-C/HGV RNA for research or diagnostic purposes should participate in multicenter quality control trials.Entities:
Mesh：
Substances：
Year: 1997 PMID： 9399538 PMCID： PMC230166 DOI： 10.1128/jcm.35.12.3298-3300.1997
Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948